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anti-S100A8 antibody :: Rat anti-Mouse S100A8 Monoclonal Antibody

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Catalog # MBS602107
Unit / Price
  0.1 mg  /  $645 +1 FREE 8GB USB
Product Name

S100A8, Monoclonal Antibody

Popular Item
Also Known As

S100A8 (MRP-8)

Product Synonym Names
Anti -S100A8 (MRP-8)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
chromosome: 1; Location: 1q21
OMIM
123885
3D Structure
ModBase 3D Structure for P27005
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8H150
Host
Rat
Species Reactivity
Mouse
Specificity
Recognizes mouse S100A8. Species Crossreactivity: Recombinant human S100A8: ~40%. No crossreactivity is observed with recombinant mouse s100A4, recombinant mouse S100A9, recombinant mouse S100A10, recombinant mouse S100A11, recombinant human S100B or recombinant human S100P.
Purity/Purification
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Concentration
~0.5 mg/ml (after reconstitution) (lot specific)
Grade
Affinity Purified
Immunogen
Recombinant protein corresponding to Met1-Glu89 (predicted) from mouse from mouse S100A8, expressed in E. coli (P27005).
Recommeded Secondary Antibodies
MBS612061 IgG, Murine X-Adsorbed (FITC) Pab Gt x Rt
Preparation and Storage
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-S100A8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-S100A8 antibody
Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10kD member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal a-helix, followed by a calcium-binding EF-hand segment, and a C-terminal a-helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 is 80% and 57% aa identical to rat and human S100A8, respectively.
Applications Tested/Suitable for anti-S100A8 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-S100A8 antibody
Western Blot: 2ug/ml using mouse leukocyte
Immunocytochemistry: 8-25 ug/ml using immersion fixed NIH-3T3 mouse embryonic fibroblast cell line

Optimal dilutions to be determined by the researcher.

Western Blot (WB) of anti-S100A8 antibody
Western Blot analysis of mouse S100A8 of lysates of mouse leukocyte. PVDF membrane was probed with S0052-17H1 (2ug/ml) followed by IgG (HRP) anti-rat IgG secondary antibody. A specific band was detected for S100A8 at ~10kD (as indicated). This experiment was conducted under reducing conditions and using the buffers listed in the protocol
anti-S100A8 antibody Western Blot (WB) (WB) image
Immunocytochemistry (ICC) of anti-S100A8 antibody
S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10ug/mL MBS602107 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).
anti-S100A8 antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for S100A8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
S100A8
NCBI Official Synonym Full Names
S100 calcium binding protein A8
NCBI Official Symbol
S100A8  [Similar Products]
NCBI Official Synonym Symbols
P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100A8
  [Similar Products]
NCBI Protein Information
protein S100-A8; MRP-8; calgranulin A; calgranulin-A; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A)
UniProt Protein Name
Protein S100-A8
UniProt Synonym Protein Names
Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; CFAG; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; S100 calcium-binding protein A8; Urinary stone protein band A
Protein Family
UniProt Gene Name
S100A8  [Similar Products]
UniProt Synonym Gene Names
CAGA; CFAG; MRP8  [Similar Products]
UniProt Entry Name
S10A8_HUMAN
NCBI Summary for S100A8
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq]
UniProt Comments for S100A8
S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; cytoskeleton; plasma membrane; extracellular region; cytosol; nucleus

Molecular Function: arachidonic acid binding; protein binding; RAGE receptor binding; zinc ion binding; microtubule binding; calcium ion binding

Biological Process: caspase activation; neutrophil chemotaxis; chronic inflammatory response; chemokine production; wound healing; cytokine production; response to lipopolysaccharide; positive regulation of peptide secretion; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; response to zinc ion; defense response to bacterium; innate immune response; autophagy; inflammatory response; defense response to fungus; acute inflammatory response; regulation of cytoskeleton organization and biogenesis; positive regulation of inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Products associated withanti-S100A8 antibody
 Reference Product  PubMed Publications
 S100A9 antibody  >477 publications with S100A8 and S100A9
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