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anti-Mouse TCR alpha beta Monoclonal Antibody

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Catalog # MBS673505
Unit / Price
  0.5 mg  /  $250 +1 FREE 8GB USB
Testing Data
Product Name

TCR alpha beta, Monoclonal Antibody

Full Product Name

Hamster Anti-Mouse TCRb-FITC

Product Synonym Names
Hamster Anti-Mouse TCRb - FITC; Hamster Anti-Mouse TCR alpha beta
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Armenian Hamster IgG
Clone Number
H57-597
Host
Armenian Hamster
Species Reactivity
Mouse
Specificity
Common epitope of the beta chain of the mouse alpha beta T cell receptor (TCR) complex
The alpha beta TCR is expressed on T lymphocytes of all mouse strains tested.2 The H57-597 monoclonal antibody does not react with gamma delta TCR-bearing cells.2 Plate-bound or soluble H57-597 activates alpha beta TCRbearing T cells.2 In vitro and in vivo, H57-597 can induce immature thymocytes to undergo apoptosis.3,4 In vivo administration of H57-597 has been shown to deplete alpha beta TCR-bearing cells to near completion and prevent graft rejection.5
Form/Format
Fluorescein (FITC) Conjugate
Immunogen
TCR affinity purified from mouse T-cell hybridoma DO-11.10
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Alexa Fluor 488 (AF488) and Alexa Fluor 647 (AF647) conjugates are supplied as 0.1mg in 0.2mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of anti-TCR alpha beta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TCR alpha beta antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
Application Notes for anti-TCR alpha beta antibody
Research Application(s): In vitro stimulation of alpha beta TCR-expressing T cells
In vitro and in vivo depletion of alpha beta TCR-bearing cells

Flow Cytometry Suggested Dilution: <= 2 ug/10^6 cells

Product References and Citations for anti-TCR alpha beta antibody
1. Gascoigne, N. R. J. 1990. J. Biol. Chem. 265:9296.
2. Kubo, R. T., W. Born, J. W. Kappler, P. Marrack, and M. Pigeon. 1989. J. Immunol. 142:2736.
3. Finkel, T. H., J. C. Cambier, R. T. Kubo, W. K. Born, P. Marrack, and J. W. Kappler. 1989. Cell 58:1047.
4. Shi, Y. F., R. P. Bissonnette, N. Parfrey, M. Szalay, R. T. Kubo, and D. R. Green. 1991. J. Immunol. 146:3340.
5. Murosaki, S., Y. Yoshikai, A. Ishida, T. Nakamura, G. Matsuzaki, H. Takimoto, H. Yuuki, and K. Nomoto. 1991. Int. Immunol. 3:1005.
6. Finkel, T. H., P. Marrack, J. W. Kappler, R. T. Kubo, and J. C. Cambier. 1989. J. Immunol. 142:3006.
7. Iwata, M., K. Katamura, R. T. Kubo, and K. Ishizaka. 1989. J. Immunol. 143:3909.
8. Iwata, M., K. Katamura, R. T. Kubo, H. M. Grey, and K. Ishizaka. 1989. J. Immunol. 143:3917.
9. Byrne, J. A., J. L. Butler, E. L. Reinherz, and M. D. Cooper. 1989. Int. Immunol. 1:29.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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