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anti-MPO antibody :: Mouse MPO Monoclonal Antibody

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Catalog # MBS584425
Unit / Price
  0.1 mg  /  $665 +1 FREE 8GB USB
anti-MPO antibody
Product Name

MPO, Monoclonal Antibody

Popular Item
Also Known As

MPO, Mouse, mAb 8F4

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
745
OMIM
606989
Clonality
Monoclonal
Isotype
Mouse IgG1
Host
Mouse
Species Reactivity
Reactivity: Mouse. Cross reactivity: Rat
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Immunogen
Purified mouse MPO from WEHI-3 cells
Negative Control
Lymphocytes isolated from digested infarcts
Positive Control
Neutrophils isolated from digested infarcts
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Other Notes
Small volumes of anti-MPO antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MPO antibody
Product Description: The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO). MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa.- MPO is- stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms.
Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer's disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an diagnostic marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an- increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient- mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice.
The anti-mouse MPO monoclonal antibody 8F4 recognizes natural MPO in biological solutions by ELISA,- in frozen tissue sections fixed with acetone and in flow cytometry using a- cell permeabilization method. The antibody 8F4 is cross-reactive- with rat MPO
Applications Tested/Suitable for anti-MPO antibody
F3,4, Flow Cytometry (FC2,5/FACS), Immunoassay (IA)1,3
Application Notes for anti-MPO antibody
Application Use: For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
NCBI/Uniprot data below describe general gene information for MPO. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
87,248 Da
NCBI Official Full Name
myeloperoxidase
NCBI Official Synonym Full Names
myeloperoxidase
NCBI Official Symbol
NCBI Protein Information
myeloperoxidase
UniProt Protein Name
Myeloperoxidase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
PERM_HUMAN
NCBI Summary for MPO
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]
UniProt Comments for MPO
MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.11.2.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q23.1

Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule

Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding

Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; hydrogen peroxide catabolic process; response to mechanical stimulus; response to lipopolysaccharide; defense response; response to yeast; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging

Disease: Myeloperoxidase Deficiency; Alzheimer Disease
Product References and Citations for anti-MPO antibody
1. Huugen, D et al; Aggravation of anti-myeloperoxidase antibody-induced glomerulonephritis by bacterial lipopolysaccharide: role of tumor necrosis factor-alpha. Am J Pathol 2005, 167: 47 2. Wang, D et al; C/EBPalpha directs monocytic commitment of primary myeloid progenitors. Blood 2006, 108:1223
3. Matthijsen, M et al; Myeloperoxidase is critically involved in the induction of organ damage after renal ischemia reperfusion. Am J Pathol 2007, 171: 1743
4. Leeuwen van, M et al; Accumulation of myeloperoxidase-positive neutrophils in atherosclerotic lesions in LDLR -/- mice. Arterioscler Thromb Vasc Biol 2008, 28: 84
5. Nahrendorf, M et al; An activatable MR imaging agent reports myeloperoxidase activity in healing infarcts and detects the anti-inflammatory effects of atorvastatin on ischemia-reperfusion injury non-invasively. Circulation 2008, 117: 1153

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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