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anti-TOP2B antibody :: Mouse anti-Mouse, Rat Topoisomerase II Monoclonal Antibody

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Catalog # MBS530015
Unit / Price
  0.1 mg  /  $670 +1 FREE 8GB USB
anti-TOP2B antibody
Product Name

Topoisomerase II (TOP2B), Monoclonal Antibody

Full Product Name

Topoisomerase II antibody

Product Synonym Names
Monoclonal Topoisomerase II; Anti-Topoisomerase II; Anti Topoisomerase II; Topoisomerase II alpha; Topoisomerase II; Topoisomerase II beta
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
126431
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AK5
Host
Mouse
Species Reactivity
Mouse, Rat
Specificity
Human
Purity/Purification
Topoisomerase II antibody was purified by affinity chromatography.
Form/Format
PBS, pH7.2, with 50% glycerol.
Biological Significance
Type II topoisomerases cut both strands of the DNA helix simultaneously in order to unwind it. The energy required for this is the hydrolysis of ATP, unlike type I topoisomerase, which is thermodynamically favored. In this process, these enzymes change the linking number of circular DNA by +/-2.
Immunogen
Topoisomerase II antibody was raised in mouse using recombinant human DNA-topoisomerase IIa (N-terminal fragment) as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-TOP2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TOP2B antibody
Mouse monoclonal Topoisomerase II antibody
Product Categories/Family for anti-TOP2B antibody
Applications Tested/Suitable for anti-TOP2B antibody
Western Blot (WB)
Application Notes for anti-TOP2B antibody
WB: 5-10 ug/ml
NCBI/Uniprot data below describe general gene information for TOP2B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Secondary Accession #
Molecular Weight
182,663 Da[Similar Products]
NCBI Official Full Name
topoisomerase II, partial
UniProt Protein Name
DNA topoisomerase 2-beta
UniProt Synonym Protein Names
DNA topoisomerase II, beta isozyme
Protein Family
UniProt Gene Name
TOP2B  [Similar Products]
UniProt Entry Name
TOP2B_HUMAN
UniProt Comments for TOP2B
TOP2B: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Belongs to the type II topoisomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Topoisomerase; DNA-binding; Motility/polarity/chemotaxis; EC 5.99.1.3; Nucleolus; Cell development/differentiation

Chromosomal Location of Human Ortholog: 3p24

Cellular Component: nucleoplasm; heterochromatin; DNA topoisomerase complex (ATP-hydrolyzing); cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: protein C-terminus binding; DNA topoisomerase (ATP-hydrolyzing) activity; enzyme binding; protein kinase C binding; protein heterodimerization activity; metal ion binding; histone deacetylase binding; chromatin binding; DNA bending activity; ATP binding

Biological Process: DNA unwinding during replication; mitotic recombination; DNA topological change; axonogenesis; forebrain development; neuron migration; sister chromatid segregation; resolution of meiotic joint molecules as recombinants
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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