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Ly-6A/E (Sca-1) Secondary Antibody

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Catalog # MBS673484
Unit / Price
  0.5 mg  /  $415 +1 FREE 8GB USB
Ly-6A/E (Sca-1) secondary antibody
Product Name

Ly-6A/E (Sca-1), Secondary Antibody

Full Product Name

Rat Anti-Mouse Ly-6A/E-LE/AF

Product Synonym Names
Rat Anti-Mouse Ly-6A/E - Low Endotoxin/Azide-Free; Rat Anti-Mouse Ly-6A/E (Sca-1)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG2a kappa
Clone Number
D7
Host
Rat
Species Reactivity
Mouse
Specificity
Ly-6A.2 and Ly-6E.1, allelic members of the Ly-6 multigene family; Mr 18 kDa
Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins.^1-3 It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes.^4-6 In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E^+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E^+ lymphocytes.^1, 7-9 The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.^1, 8, 9 Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses,^9-12 and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.^3, 13
Form/Format
Low Endotoxin, Azide - Free (LE/AF)
Immunogen
IL-2-dependent mouse T-cell line CTL-L
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of Ly-6A/E (Sca-1) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for Ly-6A/E (Sca-1) secondary antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot
Research Application(s): In vitro lymphocyte activation
Product References and Citations for Ly-6A/E (Sca-1) secondary antibody
1. Ortega, G., P.E. Korty, E.M. Shevach, and T.R. Malek. 1986. J. Immunol. 137:3240-3246.
2. Palfeee, R.G.E., F.J. Dumont, and U. Hammerling. 1986. Immunogenetics 23:197-207.
3. Rock, K.L., H. Reiser, A. Bamezai, J. McGrew, and B. Benacerraf. 1989. Immunol. Rev. 111:195-224.
4. Moore, T., M. Bennett, and V. Kumar. 1995. J. Immunol. 154:1653-1663.
5. Ito, M., K. Anan, M. Masawa, S. Kai, and H. Hara. 1996. Stem Cells 14:412-418.
6. Yonemura, Y., H. Ku, S.D. Lyman, and M. Ogawa. 1997. Blood 89: 1915-1921.
7. Codias, E.K., and C. Cray, R.D. Baler, R.B. Levy, and T.R. Malek. 1989. Immunogenetics 29:98-107.
8. Malek, T.R., K.M. Danis, and E.K. Codias. 1989. J. Immunol. 142:1929-1936.
9. Codias, E.K., and T.R. Malek. 1990. J. Immunol. 144:2197-2204.
10. Malek, T.R., G. Ortega, C. Chan, R.A. Kroczek, and E.M. Shevach. 1986. J. Exp. Med. 164:709-722.
11. Codias, E.K., J.E. Rutter, T.J. Fleming, and T.R. Malek. 1990. J. Immunol. 145:1407-1414.
12. Ivanov, V., T.J. Fleming, and T.R. Malek. 1994. J. Immunol. 153:2394-2406.
13. Flood, P.M., J.P. Dougherty, and Y. Ron. 1993. J. Exp. Med. 172:115-120.

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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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