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Ly49C/F/H/I Secondary Antibody

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Catalog # MBS673544
Unit / Price
  0.5 mg  /  $310 +1 FREE 8GB USB
Ly49C/F/H/I secondary antibody
Product Name

Ly49C/F/H/I, Secondary Antibody

Also Known As

Hamster Anti-Mouse Ly-49C/F/H/I-BIOT

Product Synonym Names
Hamster Anti-Mouse Ly-49C/F/H/I - Biotin; Hamster Anti-Mouse Ly49C/F/H/I
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Syrian Hamster IgG
Clone Number
14B11
Host
Syrian Hamster
Species Reactivity
Mouse
Specificity
An epitope common to the Ly49C, Ly49F, Ly49H and Ly49I allelic/splice variants
The monoclonal antibody 14B11 reacts with murine Ly49C, Ly49F, Ly49H and Ly49I, members of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated.2 These inhibitory receptors are also expressed on a subpopulation of mouse CD8+ T cells. The 14B11 antibody stains ~ 90% of NK1.1+CD3- splenocytes, and ~ 6% of CD8+ spleen cells from C57BL/6 mice.1 The antibody also stains 30-80% of NK1.1+CD3- splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL, AKR/J).
Form/Format
Biotin (BIOT) Conjugate
Characterization
To insure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Other Notes
Small volumes of Ly49C/F/H/I secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for Ly49C/F/H/I secondary antibody
Flow Cytometry
Application Notes for Ly49C/F/H/I secondary antibody
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Product References and Citations for Ly49C/F/H/I secondary antibody
1. Coles, M.C., C.W. McMahon, H. Takizawa, and D.H. Raulet. 2000. Memory Eur. J. Immunol. 30:236.
2. Ljunggren, H.G., and K. Karre. 1990. Immunol. Today 11:237.
3. McMahon, C., and D.H. Raulet. Personal communication.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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