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Rat Anti-Mouse Lambda (lambda light chain specific) Secondary Antibody

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Catalog # MBS673052
Unit / Price
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  1 mL  /  $295 +1 FREE 8GB USB
IMMUNOFLUORESCENT STAINING
Product Name

Rat Anti-Mouse Lambda (lambda light chain specific), Secondary Antibody

Popular Item
Full Product Name

Rat Anti-Mouse Lambda-AP

Product Synonym Names
Rat Anti-Mouse Lambda- Alkaline Phosphatase-Labeled; Rat Anti-Mouse Lambda (lambda light chain specific)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Rat IgG2b kappa
Clone Number
JC5-1
Host
Rat
Species Reactivity
Mouse
Specificity
Reacts with mouse lambda light chains as demonstrated by ELISA, FLISA, and/or flow cytometry.
Form/Format
Alkaline Phosphatase (AP) Conjugate
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry and/or ELISA to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Other Notes
Small volumes of Rat Anti-Mouse Lambda (lambda light chain specific) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
ELISA; Flow Cytometry; Immunofluorescence
Research Application(s): Identification and enumeration of lambda ^+ cells by flow cytometry
Identification and enumeration of lambda ^+ cells by immunofluorescence microscopy

ELISA:
Alkaline phosphatase conjugate: 1:1,000-1:4,000

Product: Rat Anti-Mouse lambda-R-PE
Amount Used: < or = 0.2 ug/10^6 cells
BALB/c splenocytes were double-stained with rat anti-mouse lambda-R-PE and rat anti-mouse C019-FITC (Clone 6D5). Small lymphocytes were then gated and analyzed using a flow cytometer.
Rat Anti-Mouse Lambda (lambda light chain specific) secondary antibody IMMUNOFLUORESCENT STAINING image
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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