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anti-PPARg antibody :: Mouse anti-Rat, Pig Peroxisome Proliferator Activated Receptor Gamma Monoclonal Antibody

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Catalog # MBS2032027 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $110 +1 FREE 8GB USB
  0.05 mg  /  $145 +1 FREE 8GB USB
  0.1 mg  /  $185 +1 FREE 8GB USB
  0.2 mg  /  $230 +1 FREE 8GB USB
  1 mg  /  $530 +1 FREE 8GB USB
Product Name

Peroxisome Proliferator Activated Receptor Gamma (PPARg), Monoclonal Antibody

Popular Item
Also Known As

Peroxisome Proliferator Activated Receptor Gamma (PPARg) Monoclonal Antibody

Product Synonym Names
PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3
Matching Pairs
Unconjugated Antibody: Peroxisome Proliferator Activated Receptor Gamma (MBS2032027)
Biotin Conjugated Secondary Antibody: IgG (MBS2086030)
Matching Pairs
Unconjugated Antibody: Peroxisome Proliferator Activated Receptor Gamma (MBS2032027)
FITC Conjugated Secondary Antibody: IgG (MBS2086031)
Matching Pairs
Unconjugated Antibody: Peroxisome Proliferator Activated Receptor Gamma (MBS2032027)
Biotin Conjugated Secondary Antibody: IgG (MBS2086033)
Matching Pairs
Unconjugated Antibody: Peroxisome Proliferator Activated Receptor Gamma (MBS2032027)
FITC Conjugated Secondary Antibody: IgG (MBS2086034)
Matching Pairs
Unconjugated Antibody: Peroxisome Proliferator Activated Receptor Gamma (MBS2032027)
HRP Conjugated Secondary Antibody: IgG (MBS2086035)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
477
3D Structure
ModBase 3D Structure for O88275
Clonality
Monoclonal
Isotype
IgG2b Kappa
Host
Mouse
Species Reactivity
Rat, Pig
Specificity
The antibody is a mouse monoclonal antibody raised against PPARg. It has been selected for its ability to recognize PPARg in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/ml (lot specific)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Organism Species
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2049167
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-PPARg antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PPARg antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-PPARg antibody
Western Blot: 1:100-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-PPARg antibody
Western Blot: Sample: Lane1: Porcine Liver Tissue; Lane2: Porcine Heart Tissue.
anti-PPARg antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-PPARg antibody
Western Blot: Sample: Recombinant PPARg, Rat.
anti-PPARg antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PPARg. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,471 Da
NCBI Official Full Name
peroxisome proliferator-activated receptor gamma isoform 2
NCBI Official Synonym Full Names
peroxisome proliferator-activated receptor gamma
NCBI Official Symbol
Pparg  [Similar Products]
NCBI Protein Information
peroxisome proliferator-activated receptor gamma
UniProt Protein Name
Peroxisome proliferator-activated receptor gamma
UniProt Synonym Protein Names
Nuclear receptor subfamily 1 group C member 3
UniProt Gene Name
Pparg  [Similar Products]
UniProt Synonym Gene Names
Nr1c3; PPAR-gamma  [Similar Products]
NCBI Summary for PPARg
ligand-activated transcription factor; mediates expression of genes involved in lipid metabolism [RGD, Feb 2006]
UniProt Comments for PPARg
PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 4q42

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleus; perinuclear region of cytoplasm; protein complex

Molecular Function: activating transcription factor binding; alpha-actinin binding; arachidonic acid binding; chromatin binding; DBD domain binding; DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; drug binding; enzyme binding; estrogen receptor binding; identical protein binding; LBD domain binding; ligand-dependent nuclear receptor activity; ligand-dependent nuclear receptor transcription coactivator activity; peptide binding; protein binding; protein C-terminus binding; protein heterodimerization activity; protein phosphatase binding; protein self-association; retinoid X receptor binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor binding; zinc ion binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; brown fat cell differentiation; cell fate commitment; cell maturation; cellular response to insulin stimulus; diet induced thermogenesis; epithelial cell differentiation; fat cell differentiation; fatty acid metabolic process; fatty acid oxidation; glucose homeostasis; heart development; lipoprotein transport; long-chain fatty acid transport; low-density lipoprotein receptor biosynthetic process; monocyte differentiation; negative regulation of acute inflammatory response; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of collagen biosynthetic process; negative regulation of cytokine production; negative regulation of smooth muscle cell proliferation; negative regulation of telomerase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; organ regeneration; placenta development; positive regulation of apoptosis; positive regulation of DNA binding; positive regulation of fat cell differentiation; positive regulation of fatty acid oxidation; positive regulation of oligodendrocyte differentiation; positive regulation of phagocytosis, engulfment; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of blood pressure; regulation of circadian rhythm; regulation of fat cell differentiation; regulation of gene expression; regulation of lipid metabolic process; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to caffeine; response to cold; response to dietary excess; response to drug; response to estrogen; response to food; response to light stimulus; response to lipid; response to low density lipoprotein stimulus; response to mechanical stimulus; response to organic cyclic compound; response to organic substance; response to retinoic acid; response to starvation; response to vitamin A; rhythmic process; signal transduction; steroid hormone mediated signaling; transcription, DNA-dependent; white fat cell differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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