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anti-DEFA4 antibody :: Mouse anti-Rabbit Neutrophil Defensin 5 Monoclonal Antibody

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Catalog # MBS603525
Unit / Price
  0.1 mg  /  $835 +1 FREE 8GB USB
anti-DEFA4 antibody
Product Name

Neutrophil Defensin 5 (DEFA4), Monoclonal Antibody

Popular Item
Also Known As

Neutrophil Defensin 5 (NP5)

Product Synonym Names
Anti -Neutrophil Defensin 5 (NP5)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 8; NC_000008.10 (6793345..6795786, complement). Location: 8p23
OMIM
601157
3D Structure
ModBase 3D Structure for P12838
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
6A609
Host
Mouse
Species Reactivity
Rabbit
Specificity
Recognizes natural, synthetic and recombinant rabbit Neutrophil Defensin 5. Reacts with natural NP-5 in biological solutions, in tissue sections and leukocyte smears fixed with ethanol, methanol/acetone or paraformaldehyde and in Flow Cytometry analysis of rabbit neutrophils stained by cell permeabilization method.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Concentration
0.1mg/ml (lot specific)
Immunogen
Rabbit Neutrophil Defensin 5 (NP5).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-DEFA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DEFA4 antibody
Alpha defensins belong together with the beta-defensins to the family of cationic trisulfide-containing microbicidal peptides. They exert their microbicidal activity by permeating the membrane involving the formation of voltage regulated ion channels. Rabbit neutrophils contain large amount of alpha defensins (Neutrophil Defensin) NP 1-6. Defensins constitute more than 15% of total protein in rabbit neutrophils and are accumulated in dense cytoplasmic granules. Rabbit neutrophils express all six rabbit alpha-defensins, NP 1-6. Among myeloid rabbit alpha-defensins NP 1-6, NP 3-6 are neutrophil specific, while NP 1-2 are expressed in rabbit alveolar macrophages. Defensins are relatively resistant to proteolysis, low pH and boiling. Rabbit neutrophil defensins, whose three-dimensional structures are very similar to those of human alpha defensins may be helpful in understanding the role of antimicrobial peptides in innate and acquired immunity and in the studies on the role of neutrophils in pathological situations and in various rabbit models of human diseases.
Product Categories/Family for anti-DEFA4 antibody
Applications Tested/Suitable for anti-DEFA4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-DEFA4 antibody
Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry.
Dilution: Western Blot: 1:10
Flow Cytometry: 1:10
Immunohistochemistry (Paraffin, frozen): 1:10
NCBI/Uniprot data below describe general gene information for DEFA4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
10,504 Da[Similar Products]
NCBI Official Full Name
neutrophil defensin 4 preproprotein
NCBI Official Synonym Full Names
defensin, alpha 4, corticostatin
NCBI Official Symbol
DEFA4  [Similar Products]
NCBI Official Synonym Symbols
HP4; DEF4; HP-4; HNP-4
  [Similar Products]
NCBI Protein Information
neutrophil defensin 4; corticostatin; defensin, alpha 4, preproprotein
UniProt Protein Name
Neutrophil defensin 4
UniProt Synonym Protein Names
Defensin, alpha 4; HNP-4
Protein Family
UniProt Gene Name
DEFA4  [Similar Products]
UniProt Synonym Gene Names
DEF4; HP-4  [Similar Products]
UniProt Entry Name
DEF4_HUMAN
NCBI Summary for DEFA4
Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes are clustered on chromosome 8. This gene differs from other genes of this family by an extra 83-base segment that is apparently the result of a recent duplication within the coding region. The protein encoded by this gene, defensin, alpha 4, is found in the neutrophils; it exhibits corticostatic activity and inhibits corticotropin stimulated corticosterone production. [provided by RefSeq, Jul 2008]
UniProt Comments for DEFA4
DEFA4: Has antimicrobial activity against Gram-negative bacteria, and to a lesser extent also against Gram-positive bacteria and fungi. Protects blood cells against infection with HIV-1 (in vitro). Inhibits corticotropin (ACTH)-stimulated corticosterone production. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23

Cellular Component: extracellular space; Golgi lumen; extracellular region

Biological Process: killing of cells of another organism; antibacterial humoral response; innate immune response; defense response to fungus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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