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anti-VCAM1 antibody :: Mouse anti-Rabbit Vascular Cell Adhesion Molecule 1 (VCAM1) Monoclonal Antibody

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Catalog # MBS2001285 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $110 +1 FREE 8GB USB
  0.05 mg  /  $145 +1 FREE 8GB USB
  0.1 mg  /  $185 +1 FREE 8GB USB
  0.2 mg  /  $230 +1 FREE 8GB USB
  1 mg  /  $530 +1 FREE 8GB USB
Product Name

Vascular Cell Adhesion Molecule 1 (VCAM1), Monoclonal Antibody

Also Known As

Monoclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)

Matching Pairs
Unconjugated Antibody: Vascular Cell Adhesion Molecule 1 (VCAM1) (MBS2001285)
Biotin Conjugated Secondary Antibody: IgG (MBS2086030)
Matching Pairs
Unconjugated Antibody: Vascular Cell Adhesion Molecule 1 (VCAM1) (MBS2001285)
FITC Conjugated Secondary Antibody: IgG (MBS2086031)
Matching Pairs
Unconjugated Antibody: Vascular Cell Adhesion Molecule 1 (VCAM1) (MBS2001285)
Biotin Conjugated Secondary Antibody: IgG (MBS2086033)
Matching Pairs
Unconjugated Antibody: Vascular Cell Adhesion Molecule 1 (VCAM1) (MBS2001285)
FITC Conjugated Secondary Antibody: IgG (MBS2086034)
Matching Pairs
Unconjugated Antibody: Vascular Cell Adhesion Molecule 1 (VCAM1) (MBS2001285)
HRP Conjugated Secondary Antibody: IgG (MBS2086035)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
647
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and S-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- IE LSGPPVNGRP VTVSCKVPNV YPFDXLEIEL LKGETMMKNK EFLEEEDKKS LETKSLEMTF IPTMEDTGKV LVCQAKLHID EMEFEPKQRQ STQPLFVNVA PRDIAVWVSP SSIVEEGRSV NMTCSSYGLP APKILWSRQL KNGDLQPLSE NTTLALISTK LEDSGIYVCE GINLAGKSRK EVELVIQVAP KDIQLTAFPS KSVKEGDTVI ISCTCGNVPE TWIILKKKAE TGDTVLKSID
3D Structure
ModBase 3D Structure for Q865F2
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Rabbit
Specificity
The antibody is a mouse monoclonal antibody raised against VCAM1. It has been selected for its ability to recognize VCAM1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/ml (lot specific)
Immunogen
Recombinant VCAM1 (Ile409~Thr682) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043791
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-VCAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-VCAM1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-VCAM1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-VCAM1 antibody
Western Blot: Sample: Rabbit Serum; Primary Ab: 1000 Mouse Anti-Rabbit VCAM1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
anti-VCAM1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-VCAM1 antibody
Western Blot: Sample: Recombinant VCAM1, Rabbit.
anti-VCAM1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for VCAM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
Molecular Weight
81,808 Da
NCBI Official Full Name
vascular cell adhesion protein 1
NCBI Official Symbol
VCAM1  [Similar Products]
NCBI Official Synonym Symbols
VCAM-1
  [Similar Products]
NCBI Protein Information
vascular cell adhesion protein 1
UniProt Protein Name
Adhesion molecule VCAM-1
UniProt Synonym Protein Names
Adhesion molecule VCAM-1
UniProt Gene Name
VCAM-1  [Similar Products]
NCBI Summary for VCAM1
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
UniProt Comments for VCAM1
VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: Golgi apparatus; extracellular space; microvillus; cell surface; apical part of cell; endoplasmic reticulum; early endosome; plasma membrane; integral to membrane; podosome; sarcolemma; filopodium; external side of plasma membrane

Molecular Function: integrin binding; amine oxidase activity; cell adhesion molecule binding

Biological Process: response to nicotine; extracellular matrix organization and biogenesis; chronic inflammatory response; regulation of immune response; viral reproduction; heart development; cytokine and chemokine mediated signaling pathway; cell-matrix adhesion; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; B cell differentiation; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; response to ionizing radiation; amine metabolic process; cell adhesion; leukocyte tethering or rolling; response to nutrient; acute inflammatory response; aging
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-VCAM1 antibody
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >17 publications with VCAM1 and Heart
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