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anti-CYCLIN D1 antibody :: Mouse anti-Rat CYCLIN D1 Monoclonal Antibody

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Catalog # MBS212298
Unit / Price
  0.1 mg  /  $470 +1 FREE 8GB USB
anti-CYCLIN D1 antibody
Product Name

CYCLIN D1, Monoclonal Antibody

Also Known As

MOUSE ANTI CYCLIN D1

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
295
OMIM
168461
3D Structure
ModBase 3D Structure for P24385
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CD1.1
Host
Mouse
Species Reactivity
Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Histology Positive Control Tissue
Small intestine, colon
Buffer Solution
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CYCLIN D1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CYCLIN D1 antibody
Mouse anti Cyclin D1 antibody, clone CD1.1 recognizes cyclin D1, a 295 amino acid, 36kDa nuclear protein which belongs to the highly conserved cyclin family. D type cyclins are predominantly expressed in the G1 phase of the cell cycle. Cyclin D1 study is of interest in various forms of malignancy and over expression has been linked to poor prognosis (Bartkova et al. 1995).
Applications Tested/Suitable for anti-CYCLIN D1 antibody
Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-CYCLIN D1 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat; Application Note: Membrane permeabilization is required for this application. MyBioSource recommend the use of Leucoperm
Immunohistology - Paraffin: Minimum Dilution: 1/1000; Maximum Dilution: 1/10000; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Maximum Dilution: 1/500
NCBI/Uniprot data below describe general gene information for CYCLIN D1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,729 Da
NCBI Official Full Name
G1/S-specific cyclin-D1
NCBI Official Synonym Full Names
cyclin D1
NCBI Official Symbol
CCND1  [Similar Products]
NCBI Official Synonym Symbols
BCL1; PRAD1; U21B31; D11S287E
  [Similar Products]
NCBI Protein Information
G1/S-specific cyclin-D1; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1 oncogene; PRAD1 oncogene
UniProt Protein Name
G1/S-specific cyclin-D1
UniProt Synonym Protein Names
B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene
UniProt Gene Name
CCND1  [Similar Products]
UniProt Synonym Gene Names
BCL1; PRAD1; BCL-1  [Similar Products]
UniProt Entry Name
CCND1_HUMAN
NCBI Summary for CYCLIN D1
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]
UniProt Comments for CYCLIN D1
CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Oncoprotein; Activator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; tight junction; transcriptional repressor complex; membrane; cyclin-dependent protein kinase holoenzyme complex; intracellular; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; protein complex binding; cyclin-dependent protein kinase regulator activity; protein kinase binding; transcription corepressor activity; transcription factor binding; protein kinase activity

Biological Process: fat cell differentiation; lactation; G1 DNA damage checkpoint; establishment and/or maintenance of chromatin architecture; positive regulation of cyclin-dependent protein kinase activity; negative regulation of Wnt receptor signaling pathway; re-entry into mitotic cell cycle; positive regulation of mammary gland epithelial cell proliferation; negative regulation of epithelial cell differentiation; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; response to vitamin E; response to magnesium ion; Leydig cell differentiation; response to iron ion; response to X-ray; response to corticosterone stimulus; response to drug; organ regeneration; Notch signaling pathway; transcription, DNA-dependent; unfolded protein response; mammary gland epithelial cell proliferation; response to organic nitrogen; liver development; response to ethanol; response to estrogen stimulus; cell division; mitotic cell cycle; positive regulation of protein amino acid phosphorylation; response to calcium ion; response to DNA damage stimulus; G1/S transition of mitotic cell cycle

Disease: Von Hippel-lindau Syndrome; Myeloma, Multiple
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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