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Monoclonal Antibody

What is Monoclonal Antibody Purification?

Monoclonal antibody purification involves collecting the media from the cultured hybridomas, centrifuging and filtering the media, and then using chromatographic or physiochemical fractionation methods to isolate the antibodies. Physiochemical fractionation involves using differential precipitation, size-exclusion or solid-phase binding of proteins based on size, charge or other chemical characteristics. These techniques include Size Exclusion Chromotography (SEC), ammonium sulfate precipitation and Ion Exchange Chromotography. Antibodies can also be purified based on class-specific affinity which involves using immobilized agents, such as proteins or lectins, that have an affinity for the isotype of the antibody of interest. Antigen-affinity chromatography can also be used to purify antibodies, but this method is not commonly used for monoclonal antibodies since the target antibody is generally the only immunoglobulin in the sample.

Displaying 1 to 50 (of 2044 products) order by Products Name.
Mouse Anti-Porcine B Cell Subset-UNLB
Other Names:
Mouse Anti-Porcine B Cell Subset - Purified; Unlabeled Antibody; Mouse Anti-Pig B Cell Subset
Host/Reactivities:
Host: Mouse / Reactivity: Porcine
Isotype/Clone:
Isotype: Mouse (BALB/c) IgM kappa / Clone BB6-10A10
Applications:
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections)
 
(Antibody)
Mouse Anti-Porcine B Cell Subset-FITC
Other Names:
Mouse Anti-Porcine B Cell Subset - FITC; Mouse Anti-Pig B Cell Subset
Host/Reactivities:
Host: Mouse / Reactivity: Porcine
Isotype/Clone:
Isotype: Mouse (BALB/c) IgM kappa / Clone BB6-10A10
Applications:
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections)
 
(Antibody)
Mouse Anti-Porcine B Cell Subset-BIOT
Other Names:
Mouse Anti-Porcine B Cell Subset - Biotin; Mouse Anti-Pig B Cell Subset
Host/Reactivities:
Host: Mouse / Reactivity: Porcine
Isotype/Clone:
Isotype: Mouse (BALB/c) IgM kappa / Clone BB6-10A10
Applications:
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections)
 
(Antibody)
Mouse Anti-Porcine B Cell Subset-PE
Other Names:
Mouse Anti-Porcine B Cell Subset - Phycoerythrin-Labeled; Mouse Anti-Pig B Cell Subset
Host/Reactivities:
Host: Mouse / Reactivity: Porcine
Isotype/Clone:
Isotype: Mouse (BALB/c) IgM kappa / Clone BB6-10A10
Applications:
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections)
 
(Antibody)
MOUSE ANTI GUINEA PIG B CELL SUBSET
Host/Reactivities:
Host: Mouse
Isotype/Clone:
Isotype: IgG1 / Clone MsGp10
Applications:
Immunohistology Frozen, Flow cytometry (FC/FACS)
 
(Antibody)
Anti-Pig B Cell Subset-UNLB Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Porcine
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgM kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Pig B Cell Subset-FITC Conjugate Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Porcine
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgM kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Pig B Cell Subset-Biotin Conjugate Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Porcine
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgM kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Pig B Cell Subset-R-PE Conjugate Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Porcine
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgM kappa
Applications:
Flow Cytometry (FC/FACS)
 
MOUSE ANTI HUMAN B CELLS:FITC
Gene Names:
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Other Names:
B-lymphocyte antigen CD20; membrane-spanning 4-domains; subfamily A; member 1
Host/Reactivities:
Host: Mouse
Isotype/Clone:
Isotype: IgM / Clone FMC7
Applications:
Flow cytometry (FC/FACS)
Testing Data
B Cells, 28kD
Other Names:
Anti -B Cells; 28kD
Host/Reactivities:
Host: Mouse / Reactivity: Human
Purification:
SupernatantSupernatant
Isotype/Clone:
Isotype: IgG1 / Clone MB2
Applications:
Immunohistochemistry (IHC)
 
(Antibody)
anti-B human blood group antigen IgM
Other Names:
Anti-B blood group antigen antibody; B IgM (murine monoclonal)
Host/Reactivities:
Host: Mouse hybridoma / Reactivity: Gives weak agglutination with A1 red blood cells treated with proteolytic enzymes
Purification:
Protein A purified
 
Anti-B Raf Mouse mAb
Gene Names:
B Raf; BRAF; BRAF NS7; BRAF1; RAFB1; B-RAF1; NS7; B-raf
Other Names:
FLJ95109; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF 1; BRAF_HUMAN; cRmil; MGC126806; MGC1382...
Host/Reactivities:
Host: MouseSource: Human / Reactivity: Human, Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a / Clone 4E1-E1-F9
Applications:
Western Blot (WB)
Western Blot (WB)
(Antibody)
B Raf
Gene Names:
B Raf; BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
Other Names:
Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; serine/threonine-protein kinase B-raf isoform 1; B-Raf proto-oncogene; serine/threonin...
Host/Reactivities:
Host: Rabbit / Reactivity: Human, Mouse, Rat
Purification:
ProA affinity purified
Isotype/Clone:
Isotype: IgG
Applications:
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
 
B Raf Antibody
Gene Names:
BRAF; NS7; BRAF1; RAFB1; B-RAF1
Other Names:
Purified Mouse Monoclonal Antibody (Mab); FLJ95109; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRA...
Host/Reactivities:
Host: Mouse / Reactivity: Human, Mouse
Applications:
Western Blot (WB)
Western Blot (WB)
Anti-B Raf Mouse mAb
Gene Names:
B Raf; BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
Other Names:
FLJ95109; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF 1; BRAF_HUMAN; cRmil; MGC126806; MGC1382...
Host/Reactivities:
Host: Mouse / Reactivity: Human, Mouse
Purification:
Affinity purified
Isotype/Clone:
Isotype: IgG2a / Clone 4E1-E1-F9
Applications:
Western Blot (WB)
 
Anti-B Raf Rabbit Monoclonal Antibody
Gene Names:
BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
Other Names:
Serine/threonine-protein kinase B-raf; 2.7.11.1; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; serine/threonine-protein kinase B-raf isoform 1; B-Raf proto-oncogene; serin...
Host/Reactivities:
Host: Rabbit / Reactivity: Human, Mouse, Rat
Purification:
Affinity-chromatography
Applications:
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
    (Antibody)
    Anti-Phospho-B Raf (T401) Rabbit Monoclonal Antibody
    Gene Names:
    BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
    Other Names:
    Serine/threonine-protein kinase B-raf; 2.7.11.1; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; serine/threonine-protein kinase B-raf isoform 1; B-Raf proto-oncogene; serin...
    Host/Reactivities:
    Host: Rabbit / Reactivity: Human, Mouse, Rat
    Purification:
    Affinity-chromatography
    Applications:
    Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
    Western Blot (WB)
    Phospho-B Raf (T401)
    Gene Names:
    B Raf; BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
    Other Names:
    Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; serine/threonine-protein kinase B-raf isoform 1; B-Raf proto-oncogene; serine/threonin...
    Host/Reactivities:
    Host: Rabbit / Reactivity: Human, Mouse, Rat
    Purification:
    ProA affinity purified
    Isotype/Clone:
    Isotype: IgG
    Applications:
    Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
     
    Anti-B raf Mouse mAb
    Gene Names:
    BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
    Other Names:
    FLJ95109; 94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF 1; BRAF_HUMAN; cRmil; MGC126806; MGC1382...
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Isotype/Clone:
    Isotype: IgG1 / Clone 3E8-C2-G9
    Applications:
    Western Blot (WB)
    • Western Blot (WB)
    • Immunofluorescence (IF)
    • Immunohistochemistry (IHC)
      (Antibody)
      b-2-Microglobulin
      Gene Names:
      HLA-G; MHC-G
      Other Names:
      IMD43; b2 microglobulin; major histocompatibility complex; class I; G
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      >95%
      Isotype/Clone:
      Isotype: IgG2b / Clone BBM.1
      Applications:
      Blocking (BL), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
       
      (Antibody)
      Anti - b-Actin
      Gene Names:
      b-Actin; ACTB; BRWS1; PS1TP5BP1
      Other Names:
      Actin; cytoplasmic 1; beta
      Host/Reactivities:
      Host: Rabbit / Reactivity: Human, mouse, rat
      Applications:
      Immunohistochemistry (IHC) - Formalin/Paraffin
      Testing Data
      Anti-b-actin
      Gene Names:
      b-actin
      Other Names:
      Anti-beta-Actin monoclonal antibody(Mouse IgG2b)
      Host/Reactivities:
      Host: Mouse
      Applications:
      ELISA (EIA), Western Blot (WB)
      Testing Data
      b-Amyloid (1-40), CT (FITC)
      Other Names:
      Anti -b-Amyloid (1-40); CT (FITC)
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      Affinity PurifiedPurified by subsequent thiophilic adsorption and size exclusion chromatography.
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D3
      Applications:
      ELISA (EL/EIA), Immunocytochemistry (ICC)
       
      b-Amyloid, NT
      Other Names:
      Anti -b-Amyloid; NT
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      PurifiedPurified by subsequent thiophillic adsorption and size exclusion chromatography.
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D2
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
       
      b-Amyloid (1-42), CT (FITC)
      Other Names:
      Anti -b-Amyloid (1-42); CT (FITC)
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      Affinity PurifiedPurified by subsequent thiophilic adsorption and size exclusion chromatography.
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D5
      Applications:
      ELISA (EL/EIA), Immunocytochemistry (ICC)
       
      (Antibody)
      b-Amyloid, NT
      Other Names:
      Anti -b-Amyloid; NT
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      Affinity PurifiedHighly purified from mouse ascites by protein A chromatography.
      Isotype/Clone:
      Isotype: IgG2a,k / Clone 4H304
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
       
      (Antibody)
      b-Amyloid, NT (Biotin)
      Other Names:
      Anti -b-Amyloid; NT (Biotin)
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D1
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
       
      (Antibody)
      b-Amyloid (1-40), CT (Biotin)
      Other Names:
      Anti -b-Amyloid (1-40); CT (Biotin)
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      Purified by subsequent thiophilic adsorption and size exclusion chromatography.
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D3
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
       
      (Antibody)
      b-Amyloid, NT
      Other Names:
      Anti -b-Amyloid; NT
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      PurifiedPurified by subsequent thiophillic adsorption and size exclusion chromatography.
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D1
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
       
      (Antibody)
      b-Amyloid (1-42), CT
      Other Names:
      Anti -b-Amyloid (1-42); CT
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      PurifiedPurified by subsequent thiophilic adsorbtion and size exclusion chromatography.
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D5
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
       
      (Antibody)
      b-Amyloid
      Other Names:
      Anti -b-Amyloid
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      Affinity PurifiedPurified by Protein G affinity chromatography.
      Isotype/Clone:
      Isotype: IgG1 / Clone 3H532
      Applications:
      ELISA (EL/EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC)
       
      (Antibody)
      b-Amyloid (1-43), CT
      Other Names:
      Anti -b-Amyloid (1-43); CT
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D116
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
       
      (Antibody)
      b-Amyloid (1-40), CT
      Other Names:
      Anti -b-Amyloid (1-40); CT
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      PurifiedPurified by subsequent thiophilic adsorption and seize exclusion chromatgraphy.
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D3
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
       
      (Antibody)
      b-Amyloid 1-40, 42, CT
      Other Names:
      Anti -b-Amyloid 1-40; 42; CT
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      PurifiedPurified by size exclusion chromatography.Grade: Highly Purified
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D4
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC).Other applications have not been tested.
       
      (Antibody)
      Popular Item
      b-Amyloid 1-40, 42, CT (Biotin)
      Other Names:
      Anti -b-Amyloid 1-40; 42; CT (Biotin)
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      PurifiedPurified by subsequent thiophillic adsorption and size exclusion chromatography.
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D4
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
       
      (Antibody)
      b-Amyloid 1-40, 42, CT (FITC)
      Other Names:
      Anti -b-Amyloid 1-40; 42; CT (FITC)
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Isotype/Clone:
      Isotype: IgG1 / Clone 6D4
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
       
      (Antibody)
      b-Amyloid 1-40, 42
      Other Names:
      Anti -b-Amyloid 1-40; 42
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      AscitesAscites
      Isotype/Clone:
      Isotype: IgG1 / Clone 3H2
      Applications:
      ELISA (EL/EIA), Western Blot (WB)
       
      (Antibody)
      b-Amyloid Peptide
      Other Names:
      Anti -b-Amyloid Peptide
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      PurifiedPuri ed
      Isotype/Clone:
      Isotype: IgG1,k / Clone 3G5
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
       
      (Antibody)
      b-Amyloid Peptide, 28aa
      Other Names:
      Anti -b-Amyloid Peptide; 28aa
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      PurifiedPurified from mouse ascites fluid
      Isotype/Clone:
      Isotype: IgG1, k / Clone 6A5
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
       
      (Antibody)
      b-Amyloid Precursor Protein (695aa)
      Other Names:
      Anti -b-Amyloid Precursor Protein (695aa)
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      PurifiedPurified
      Isotype/Clone:
      Isotype: IgG1,k / Clone 6A6
      Applications:
      ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
       
      (Antibody)
      b-Amyloid x-40 (Abx-40)
      Other Names:
      Anti -b-Amyloid x-40 (Abx-40)
      Host/Reactivities:
      Host: Mouse / Reactivity: Human, Mouse
      Purification:
      AscitesAscites
      Isotype/Clone:
      Isotype: IgG / Clone 4H308
      Applications:
      Western Blot (WB), Immunohistochemistry (IHC)
       
      (Antibody)
      b-Amyloid, aa5-16
      Other Names:
      Anti -b-Amyloid; aa5-16
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      Affinity PurifiedPurified by Protein A/G affinity chromatography.
      Isotype/Clone:
      Isotype: IgG2a,k / Clone 6F23
      Applications:
      ELISA (EL/EIA)
       
      (Antibody)
      b-Amyloid, aa5-16
      Other Names:
      Anti -b-Amyloid; aa5-16
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      Affinity PurifiedPurified by Protein A/G affinity chromatography.
      Isotype/Clone:
      Isotype: IgG1,k / Clone 6F25
      Applications:
      ELISA (EL/EIA)
       
      (Antibody)
      b-Amyloid, aa8-17
      Other Names:
      Anti -b-Amyloid; aa8-17
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      SupernatantTissue culture supernatant
      Isotype/Clone:
      Isotype: IgG1,k / Clone 0.N.28
      Applications:
      Immunohistochemistry (IHC)
       
      (Antibody)
      Anti-beta-Catenin
      Gene Names:
      ctnnb1; ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01
      Other Names:
      b-Catenin; Catenin beta; catenin (cadherin-associated protein); beta 1
      Host/Reactivities:
      Host: Mouse / Reactivity: Human, Rat, Mouse
      Purification:
      Protein A Purified
      Isotype/Clone:
      Isotype: IgG1
      Applications:
      ELISA, Western Blot, Immunocytochemistry, Immunoprecipitation
      Testing Data
      (Antibody)
      Mouse Anti Human B-cell Activating Factor
      Gene Names:
      BAFF; TNFSF13B; DTL; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
      Other Names:
      BAFF Antibody; B-cell Activating Factor; Mouse Anti Human; tumor necrosis factor ligand superfamily member 13B isoform 2; tumor necrosis factor (ligand) superfamily; member 13b
      Host/Reactivities:
      Host: Mouse
      Purification:
      BAFF antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
      Isotype/Clone:
      Isotype: IgG3 heavy chain and kappa light chain / Clone PH4C7AT
      Applications:
      ELISA (EIA), Western Blot (WB)
       
      Biotin-Linked Antibody to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L)
      Gene Names:
      Bcl2L
      Host/Reactivities:
      Reactivity: Rat
      Applications:
      Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
       
      APC/CY7-Linked Monoclonal Antibody to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L)
      Gene Names:
      Bcl2L
      Host/Reactivities:
      Host: Mouse / Reactivity: Rat
      Applications:
      Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA (EIA)
       
      Displaying 1 to 50 (of 2044 products)
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