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Monoclonal Antibody

What is Monoclonal Antibody Purification?

Monoclonal antibody purification involves collecting the media from the cultured hybridomas, centrifuging and filtering the media, and then using chromatographic or physiochemical fractionation methods to isolate the antibodies. Physiochemical fractionation involves using differential precipitation, size-exclusion or solid-phase binding of proteins based on size, charge or other chemical characteristics. These techniques include Size Exclusion Chromotography (SEC), ammonium sulfate precipitation and Ion Exchange Chromotography. Antibodies can also be purified based on class-specific affinity which involves using immobilized agents, such as proteins or lectins, that have an affinity for the isotype of the antibody of interest. Antigen-affinity chromatography can also be used to purify antibodies, but this method is not commonly used for monoclonal antibodies since the target antibody is generally the only immunoglobulin in the sample.

Displaying 1 to 50 (of 2378 products) order by Products Name.
E Cadherin antibody
Gene Names:
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Other Names:
Monoclonal E Cadherin; Anti-E Cadherin; Cadherin 1; Type 1 E-Cadherin; E Cadherin; E-cadherin; partial; type 1
Host/Reactivities:
Host: Mouse / Reactivity: Human
Isotype/Clone:
Isotype: IgG1 / Clone SEC21
Applications:
Western Blot (WB)
 
E Cadherin
Gene Names:
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
Other Names:
E-cadherin; Cadherin-1; CAM 120/80; Epithelial cadherin; Uvomorulin; cadherin-1 isoform 2; cadherin 1
Host/Reactivities:
Host: Rabbit / Reactivity: Human
Purification:
ProA affinity purified
Isotype/Clone:
Isotype: IgG
Applications:
WB, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
 
E Cadherin antibody
Gene Names:
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Other Names:
Monoclonal E Cadherin; Anti-E Cadherin; Cadherin 1; Type 1 E-Cadherin; E Cadherin; E-cadherin; partial; type 1
Host/Reactivities:
Host: Mouse / Reactivity: Human
Isotype/Clone:
Isotype: IgG1 / Clone 5H9
Applications:
Immunohistochemistry (IHC), Immunohistochemistry (IHC) Formalin, Western Blot (WB)
 
E Cadherin antibody
Gene Names:
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Other Names:
Monoclonal E Cadherin; Anti-E Cadherin; Cadherin 1; Type 1 E-Cadherin; E Cadherin; E-cadherin; partial; type 1
Host/Reactivities:
Host: Mouse / Reactivity: Human, Zebrafish
Isotype/Clone:
Isotype: IgG2b / Clone MB2
Applications:
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunohistochemistry (IHC) Formalin, Western Blot (WB)
 
Anti-E Cadherin Rabbit Monoclonal Antibody
Gene Names:
TGFBI; CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
Other Names:
Transforming growth factor-beta-induced protein ig-h3; Beta ig-h3; Kerato-epithelin; RGD-containing collagen-associated protein; RGD-CAP; transforming growth factor beta induced
Host/Reactivities:
Host: Rabbit / Reactivity: Human
Purification:
Affinity-chromatography
Applications:
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
    E Cadherin antibody
    Gene Names:
    CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
    Other Names:
    Monoclonal E Cadherin; Anti-E Cadherin; Cadherin 1; Type 1 E-Cadherin; E Cadherin; E-cadherin; partial; type 1
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Isotype/Clone:
    Isotype: IgG1 / Clone 6F9
    Applications:
    Immunohistochemistry (IHC), Immunohistochemistry (IHC) Formalin, Western Blot (WB)
     
    Anti-E Cadherin Mouse mAb
    Gene Names:
    ECAD; CDH1; UVO; CDHE; LCAM; Arc-1; CD324
    Other Names:
    Arc 1; CADH1_HUMAN; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD 324; CD324 antigen; E-Cad/CTF3; E-cadherin; Epithelial cadherin; epithelial calcium dependant adhesion p...
    Host/Reactivities:
    Host: MouseSource: Human / Reactivity: Human, Rat
    Isotype/Clone:
    Isotype: IgG1 / Clone 7H12-E4-F5
    Applications:
    Western Blot (WB)
    Western Blot (WB)
    E. coli Monoclonal Antibody
    Other Names:
    Anti-E. coli; E. coli
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG1
    Applications:
    Immunoassay
     
    E Coli antibody
    Other Names:
    Monoclonal E Coli; Anti-E Coli; Escherichia Coli
    Host/Reactivities:
    Host: Mouse / Reactivity: Specifically recognized E.coli serotypes O2a/2b, O7, O11, O18, O44, O75, O112 and O125.
    Isotype/Clone:
    Isotype: IgG1 / Clone M2110120
    Applications:
    Suitale for use in immunoassays to identifiy E.coli cells.
     
    E Coli antibody
    Other Names:
    Monoclonal E Coli; Anti-E Coli; Escherichia Coli
    Host/Reactivities:
    Host: Mouse / Reactivity: Reacts with several serotypes, including O18, O44, O112 & O125. Does not react with other members of...
    Purification:
    > 90% pure
    Isotype/Clone:
    Isotype: IgG1 / Clone 1116501
    Applications:
    ELISA (EIA)
     
    E Coli antibody
    Other Names:
    Monoclonal E Coli; Anti-E Coli; E Coli Shigatoxin; Escherichia Coli
    Host/Reactivities:
    Host: Mouse / Reactivity: E Coli
    Isotype/Clone:
    Isotype: IgG / Clone M0201113
    Applications:
    User optimized
     
    E Coli antibody
    Other Names:
    Monoclonal E Coli; Anti-E Coli; Escherichia Coli
    Host/Reactivities:
    Host: Mouse / Reactivity: E Coli
    Isotype/Clone:
    Isotype: IgG2b / Clone M50336
    Applications:
    ELISA (EIA)
     
    E Coli antibody (K99 Pilus)
    Other Names:
    Monoclonal E Coli (K99 Pilus); Anti-E Coli (K99 Pilus); K99 Pilus; Escherichia Coli
    Host/Reactivities:
    Host: Mouse / Reactivity: E Coli
    Isotype/Clone:
    Isotype: IgG1 / Clone M2110124
    Applications:
    ELISA (EIA)
     
    E. coli Monoclonal Antibody
    Other Names:
    Anti-E. coli; E. coli
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgM
    Applications:
    Immunoassay
     
    E. coli Monoclonal Antibody
    Other Names:
    Anti-E. coli; E. coli
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG1
    Applications:
    Immunoassay
     
    E. coli Monoclonal Antibody
    Other Names:
    Anti-E. coli; E. coli
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG1
    Applications:
    Immunoassay
     
    E. coli Monoclonal Antibody
    Other Names:
    Anti-E. coli; E. coli
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2b
    Applications:
    Immunoassay
     
    E Coli Monoclonal Antibodies
    Other Names:
    E.coli-Library Pack
    Host/Reactivities:
    Host: Mouse
    Applications:
    ELISA (EIA)
     
    E. coli K99 Pilus Monoclonal Antibody
    Other Names:
    Anti-E. coli K99 pili; E. coli K99 pili
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG1
    Applications:
    Immunoassay
     
    E. coli K99 Pilus Monoclonal Antibody
    Other Names:
    Anti-E. coli K99 pili; E. coli K99 pili
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG1
    Applications:
    Immunoassay
     
    E. coli K99 Pilus Monoclonal Antibody
    Other Names:
    Anti-E. coli K99 pili; E. coli K99 pili
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG1
    Applications:
    Immunoassay
     
    E. coli K99 Pilus Monoclonal Antibody
    Other Names:
    Anti-E. coli K99 pili; E. coli K99 pili; Anti-E coli K99 Pilis Monoclonal Antibody
    Host/Reactivities:
    Host: Mouse
    Purification:
    Purified E.coli K99 (B44) pili.
    Isotype/Clone:
    Isotype: IgG1
    Applications:
    These antibodies have been qualified for use in ELISA to detect K99- E.coli.
     
    E Coli K99 Pilus Monoclonal Antibodies
    Other Names:
    E Coli K99 pili-Library Pack
    Host/Reactivities:
    Host: Mouse
    Applications:
    ELISA (EIA)
     
    E Coli O157 antibody
    Other Names:
    Monoclonal E Coli O157; Anti-E Coli O157; E Coli; Escherichia Coli O157
    Host/Reactivities:
    Host: Mouse / Reactivity: This antibody recognizes the 'O' antigen of E coli serotype O157 Non-reactive with E coli serotypes:...
    Purification:
    > 90% pure
    Isotype/Clone:
    Isotype: IgG3 / Clone 1516501
    Applications:
    ELISA (EIA), Immunofluorescence (IF)
     
    MAb to E. coli O157
    Other Names:
    E. coli O157
    Host/Reactivities:
    Host: Host: MouseSource: Ascites
    Purification:
    >90% pure. Protein A chromatography
    Isotype/Clone:
    Isotype: IgG1 / Clone B835M
    Applications:
    ELISA, Immunofluorescence, Paired Antibody
     
    MAb to E. coli O157
    Other Names:
    E. coli O157
    Host/Reactivities:
    Host: Host: MouseSource: Ascites
    Purification:
    >90% pure. Protein A chromatography
    Isotype/Clone:
    Isotype: IgG1 / Clone B837M
    Applications:
    IFA, ELISA
     
    MAb to E. coli O157
    Other Names:
    E. coli O157
    Host/Reactivities:
    Host: Host: MouseSource: Ascites
    Purification:
    >90% pure. Protein A chromatography
    Isotype/Clone:
    Isotype: IgG2b / Clone B836M
    Applications:
    ELISA, Immunofluorescence, Paired Antibody
     
    Mouse monoclonal E Coli O157 antibody
    Other Names:
    E Coli O157 antibody
    Host/Reactivities:
    Host: Mouse
    Purification:
    Purified by protein A chromatography
    Isotype/Clone:
    Isotype: IgG1 / Clone M121024EC
    Applications:
    ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
     
    Mouse monoclonal E Coli O157 antibody
    Other Names:
    E Coli O157 antibody
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG1 / Clone M60711
    Applications:
    ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
     
    E Coli O157 antibody (FITC)
    Other Names:
    Monoclonal E Coli O157; Anti-E Coli O157; Escherichia Coli; E Coli
    Host/Reactivities:
    Host: Mouse / Reactivity: This antibody recognizes the 'O' antigen of E coli serotype O157
    Purification:
    > 90% pure
    Isotype/Clone:
    Isotype: IgG3 / Clone 1316501
    Applications:
    ELISA (EIA), Immunofluorescence (IF)
     
    E Coli O157 antibody
    Other Names:
    Monoclonal E Coli O157; Anti-E Coli O157; E Coli; Escherichia Coli
    Host/Reactivities:
    Host: Mouse / Reactivity: This antibody recognizes the 'O' antigen of E coli serotype O157
    Purification:
    > 90% pure
    Isotype/Clone:
    Isotype: IgG3 / Clone M02061323
    Applications:
    ELISA (EIA), Immunofluorescence (IF)
     
    MAb to E. coli O157
    Other Names:
    E. coli O157
    Host/Reactivities:
    Host: Host: MouseSource: Ascites
    Purification:
    >90% pure. Protein A chromatography
    Isotype/Clone:
    Isotype: IgG1 / Clone B838M
    Applications:
    ELISA, Immunofluorescence, Paired Antibody
     
    E Coli O157 antibody (FITC)
    Other Names:
    Monoclonal E Coli O157; Anti-E Coli O157; Escherichia Coli O157; E Coli
    Host/Reactivities:
    Host: Mouse / Reactivity: This antibody recognizes the 'O' antigen of E coli serotype O157 Non-reactive with E coli serotypes:...
    Purification:
    > 90% pure
    Isotype/Clone:
    Isotype: IgG3 / Clone 1616501
    Applications:
    ELISA (EIA), Immunofluorescence (IF)
     
    MAb to E. coli O157
    Other Names:
    E. coli O157
    Host/Reactivities:
    Host: Host: MouseSource: Ascites
    Purification:
    >90% pure. Protein A chromatography
    Isotype/Clone:
    Isotype: IgG3 / Clone B839M
    Applications:
    ELISA, Immunofluorescence, Paired Antibody
     
    Purified anti-E. coli RNA NusA Antibody
    Other Names:
    L factor; N utilization substance protein A; Transcription elongation protein NusA
    Host/Reactivities:
    Reactivity: E Coli
    Isotype/Clone:
    Isotype: IgG1 / Clone 1NA2
    Applications:
    Western Blot (WB), ELISA (EIA)
     
    HRP anti-E Coli RNA Polymerase beta Prime Antibody
    Other Names:
    RNA pol beta prime; rpoC; tabB; DNA-directed RNA polymerase subunit beta prime; RNAP subunit beta prime; RNA polymerase subunit beta prime; Transcriptase subunit beta prime
    Host/Reactivities:
    Reactivity: E Coli, Enterics, Borellia burgdorferi, Salmonella
    Isotype/Clone:
    Isotype: IgG1 / Clone NT73
    Applications:
    Western Blot (WB), ELISA (EIA)
     
    Anti-E Coli RNA Polymerase ImmunoAffinity Resin
    Other Names:
    Softag 1
    Host/Reactivities:
    Reactivity: E Coli strain MG1655, Enterics, Borellia burgdorferi, Salmonella
    Isotype/Clone:
    Isotype: IgG1 / Clone NT73
    Applications:
    Immunoprecipitation (IP)
     
    HRP anti-E Coli RNA Sigma 70 Antibody
    Other Names:
    RNA pol sigma 70; rpoD; sigma D
    Host/Reactivities:
    Reactivity: E Coli, Chlamydia trachomatis, Mycobacterium smegmatis, Staphylococcus aureus, Bordetella pertussis,...
    Isotype/Clone:
    Isotype: IgG2b / Clone 2G10
    Applications:
    Western Blot (WB), ELISA (EIA), Functional Assay, Immunoprecipitation (IP)
     
    E tag
    Host/Reactivities:
    Host: Mouse
    Purification:
    ProG affinity purified
    Isotype/Clone:
    Isotype: IgG1 / Clone B2-E2
    Applications:
    Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC)
     
    E Tag Antibody
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG1
    Applications:
    Anti-E tag can be used in ELISA and WB-Re.
    Testing Data
    Mouse monoclonal E Tag antibody
    Other Names:
    E Tag antibody
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG1 / Clone M78827
    Applications:
    ELISA (EIA), Western Blot (WB)
    Western Blot (WB)
    Anti-E-Cadherin Antibody [ABT-CDHE]
    Gene Names:
    CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
    Other Names:
    Anti-E-Cadherin [ABT-CDHE]; Mouse monoclonal [ABT-CDHE] antibody to CDHE; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3 CDH1; cadherin-1 isofo...
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Purification:
    Immunogen Affinity Purified
    Isotype/Clone:
    Isotype: IgG / Clone ABT-CDHE
    Applications:
    Immunohistochemistry (IHC), Western Blot (WB)
    • Immunohistochemistry (IHC)
    • Immunohistochemistry (IHC)
    • Immunohistochemistry (IHC)
    • Immunohistochemistry (IHC)
    • Immunohistochemistry (IHC)
    • Immunohistochemistry (IHC)
    • Immunohistochemistry (IHC)
      E-cadherin Monoclonal Antibody
      Gene Names:
      CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
      Other Names:
      cadherin-1 preproprotein; cadherin 1; type 1
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Isotype/Clone:
      Isotype: Mouse IgG1, Kappa / Clone ABM43F8
      Applications:
      Western Blot (WB), Immunohistochemistry (IHC)
      • Western Blot (WB)
      • Immunohistochemistry (IHC)
        E-cadherin
        Gene Names:
        Cdh1; Um; UVO; Ecad; ARC-1; E-cad; L-CAM; AA960649
        Other Names:
        E-cadherin; Cadherin-1; Epithelial cadherin; Uvomorulin; CD324; cadherin-1 preproprotein; cadherin 1
        Host/Reactivities:
        Host: Mouse / Reactivity: Human, Mouse
        Purification:
        ProG affinity purified
        Isotype/Clone:
        Isotype: IgG2b / Clone 3-F9
        Applications:
        Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
         
        Anti-E-Cadherin Antibody
        Gene Names:
        CDH1; CDHE; UVO; ECAD; LCAM; Arc-1; CD324
        Other Names:
        Arc 1; CADH1_HUMAN; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 324; CD324 antigen; E-Cad/CTF3; E-cadherin; Epithelial cadherin; epithelial calcium dependant adhesion protein; ...
        Host/Reactivities:
        Host: Mouse / Reactivity: Human, Mouse
        Isotype/Clone:
        Isotype: IgG1
        Applications:
        Western Blot (WB)
        • Western Blot (WB)
        • Western Blot (WB)
          E-cadherin Monoclonal Antibody
          Gene Names:
          CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
          Other Names:
          Arc 1; Cadherin 1; CAM 120/80; E cadherin; E-cadherin; Epithelial cadherin; Uvomorulin; Homo sapiens cadherin 1; type 1; E-cadherin (epithelial); mRNA
          Host/Reactivities:
          Host: Mouse / Reactivity: Human, Mouse
          Purification:
          Protein A Purification
          Isotype/Clone:
          Isotype: IgG2b / Clone 6B11F11
          Applications:
          Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
          Western Blot (WB)
          E-Cadherin Monoclonal Antibody
          Gene Names:
          CDHE; Cdh1; Um; UVO; Ecad; ARC-1; E-cad; L-CAM; AA960649
          Other Names:
          Arc 1; CADH1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 324; CD324; CD324 antigen; E-Cad/CTF3; E-cadherin; Epithelial cadherin; epithelial calcium dependant adhesion protein;...
          Host/Reactivities:
          Host: Mouse / Reactivity: Mouse, Rat
          Purification:
          Affinity Purification
          Isotype/Clone:
          Isotype: IgG / Clone EC125D
          Applications:
          Immunohistochemistry (IHC)
          • Immunohistochemistry (IHC)
          • Immunohistochemistry (IHC)
          • Immunohistochemistry (IHC)
            Anti-E-Cadherin Mouse mAb
            Gene Names:
            ECAD; CDH1; UVO; CDHE; LCAM; Arc-1; CD324.; CD324
            Other Names:
            Arc 1; CADH1_HUMAN; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 324; CD324 antigen; E-Cad/CTF3; E-cadherin; Epithelial cadherin; epithelial calcium dependant adhesion protein; ...
            Host/Reactivities:
            Host: MouseSource: Human / Reactivity: Human, Mouse
            Purification:
            Affinity Purified
            Isotype/Clone:
            Isotype: IgG1 / Clone 6B10-F4-G10
            Applications:
            Western Blot (WB)
            • Western Blot (WB)
            • Western Blot (WB)
              E-cadherin Antibody
              Gene Names:
              CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
              Other Names:
              E-cadherin; partial; cadherin 1
              Host/Reactivities:
              Host: Mouse / Reactivity: Human
              Isotype/Clone:
              Isotype: IgG / Clone IHC564
              Applications:
              Immunohistochemistry (IHC) Paraffin
              Immunohistochemistry (IHC)
              E-cadherin Antibody
              Gene Names:
              CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
              Other Names:
              Arc 1; CADH1_HUMAN; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 324; CD324 antigen; E-Cad/CTF3; E-cadherin; Epithelial cadherin; epithelial calcium dependant adhesion protein; ...
              Host/Reactivities:
              Host: Mouse / Reactivity: Human, Mouse, Monkey
              Purification:
              Affinity-Chromatography
              Isotype/Clone:
              Isotype: IgG1
              Applications:
              Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
              Western Blot (WB)
              Displaying 1 to 50 (of 2378 products)
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