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Monoclonal Antibody

What is Monoclonal Antibody Purification?

Monoclonal antibody purification involves collecting the media from the cultured hybridomas, centrifuging and filtering the media, and then using chromatographic or physiochemical fractionation methods to isolate the antibodies. Physiochemical fractionation involves using differential precipitation, size-exclusion or solid-phase binding of proteins based on size, charge or other chemical characteristics. These techniques include Size Exclusion Chromotography (SEC), ammonium sulfate precipitation and Ion Exchange Chromotography. Antibodies can also be purified based on class-specific affinity which involves using immobilized agents, such as proteins or lectins, that have an affinity for the isotype of the antibody of interest. Antigen-affinity chromatography can also be used to purify antibodies, but this method is not commonly used for monoclonal antibodies since the target antibody is generally the only immunoglobulin in the sample.

Displaying 1 to 50 (of 4828 products) order by Products Name.
anti-H ab human blood antigen IgM
Other Names:
Anti-Ho blood antigen antibody; anti-H IgM (murine monoclonal)
Host/Reactivities:
Host: Mouse hybridoma
Purification:
Protein A purified
 
anti-H inh human blood antigen IgM
Other Names:
Anti-Hinh blood antigen antibody; anti-H IgM (murine monoclonal)
Host/Reactivities:
Host: Mouse hybridoma
Purification:
Protein A purified.
 
anti-H n/ab human blood antigen IgM
Other Names:
Anti-Hn/ab blood antigen antibody; anti-H IgM (murine monoclonal)
Host/Reactivities:
Host: Mouse hybridoma
Purification:
Protein A purified.
 
H+K+ ATPase antibody
Other Names:
Monoclonal H; K ATPase; ATPase H+/K+; Anti H; Hydrogen/ Potassium ATPase beta
Host/Reactivities:
Host: Mouse / Reactivity: Bovine, Dog, Human, Mouse, Pig, Rabbit, Rat, Weasel
Isotype/Clone:
Isotype: IgG1 / Clone 2G11
Applications:
ChIP, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Immunohistochemistry (IHC)
(Antibody)
Anti-Mouse H-2 Antibody (APC conjugated)
Gene Names:
H-2; he2; hce2; wu:fb77c08; si:ch211-246m6.9
Other Names:
hatching enzyme 2
Host/Reactivities:
Host: Rat / Reactivity: Mouse
Isotype/Clone:
Isotype: IgG2a / Clone M1/42.3.9.8
 
Anti-Mouse H-2 Antibody (BIOT conjugated)
Gene Names:
H-2; he2; hce2; wu:fb77c08; si:ch211-246m6.9
Other Names:
hatching enzyme 2
Host/Reactivities:
Host: Rat / Reactivity: Mouse
Isotype/Clone:
Isotype: IgG2a / Clone M1/42.3.9.8
Applications:
FCM
 
LE/AF Purified Anti-Mouse H-2 Antibody
Gene Names:
H-2; he2; hce2; wu:fb77c08; si:ch211-246m6.9
Other Names:
hatching enzyme 2
Host/Reactivities:
Reactivity: Mouse
Isotype/Clone:
Isotype: IgG2a / Clone M1/42.3.9.8
 
Anti-Mouse H-2 Antibody (PE conjugated)
Gene Names:
H-2; he2; hce2; wu:fb77c08; si:ch211-246m6.9
Other Names:
hatching enzyme 2
Host/Reactivities:
Host: Rat / Reactivity: Mouse
Isotype/Clone:
Isotype: IgG2a / Clone M1/42.3.9.8
 
Anti-Mouse H-2 Antibody (FITC conjugated)
Gene Names:
H-2; he2; hce2; wu:fb77c08; si:ch211-246m6.9
Other Names:
hatching enzyme 2
Host/Reactivities:
Host: Rat / Reactivity: Mouse
Isotype/Clone:
Isotype: IgG2a / Clone M1/42.3.9.8
 
H-2Db antibody (PE)
Gene Names:
H-2Db
Other Names:
Monoclonal H-2Db; Anti-H-2Db; H2-Db; MHC class I H-2Dd; H-Db 2; H-Db-2; H2Db
Host/Reactivities:
Host: Mouse / Reactivity: Murine
Isotype/Clone:
Isotype: IgG2b kappa / Clone KH95
Applications:
Flow Cytometry (FC/FACS)
 
(Antibody)
Anti-Mouse H-2Db, Affinity Purified (Clone B22-249.R1) (mouse IgG2a)
Gene Names:
H-2Db
Other Names:
Affinity Purified (Clone B22-249.R1) (mouse IgG2a); Purified Anti- Mouse H-2Db Monoclonal Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Isotype/Clone:
Isotype: Mouse IgG2a /kappa / Clone B22-249.R1
Applications:
Cytotoxic Assay (CA)
Testing Data (TD)
Anti-Mouse H-2Db, FITC (Clone B22-249.R1) (mouse IgG2a)
Gene Names:
H-2Db
Other Names:
FITC (Clone B22-249.R1) (mouse IgG2a)
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Isotype/Clone:
Isotype: Mouse IgG2a / Clone B22-249.R1
Applications:
Cytotoxic Assay (CA)
 
(Antibody)
Anti-Mouse H-2Db-UNLB Antibody
Gene Names:
H-2Db
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Mouse H-2Db-FITC Conjugate Antibody
Gene Names:
H-2Db
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
Testing Data
Anti-Mouse H-2Db-Biotin Conjugate Antibody
Gene Names:
H-2Db
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Mouse H-2Db-R-PE Conjugate Antibody
Gene Names:
H-2Db
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Mouse H-2Db-LE/AF Antibody
Gene Names:
H-2Db
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Mouse H-2Dd-UNLB Antibody
Gene Names:
H-2Dd
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Mouse H-2Dd-FITC Conjugate Antibody
Gene Names:
H-2Dd
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Mouse H-2Dd-Biotin Conjugate Antibody
Gene Names:
H-2Dd
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Mouse H-2Dd-R-PE Conjugate Antibody
Gene Names:
H-2Dd
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Mouse H-2Dd (private; H-2.m4), Biotin (Clone 34-5-8S) (mouse IgG2a)
Other Names:
H-2Dd (private; H-2.m4); Biotin (Clone 34-5-8S) (mouse IgG2a); Biotin Anti-Mouse H-2Dd Monoclonal Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Isotype/Clone:
Isotype: Mouse IgG2a / Clone 34-5-8S
Applications:
Immunohistochemistry (IHC)
Testing Data
(Antibody)
Anti-Mouse H-2Dd (private; H-2.m4), FITC (Clone 34-5-8S) (mouse IgG2a)
Other Names:
H-2Dd (private; H-2.m4); FITC (Clone 34-5-8S) (mouse IgG2a); FITC Anti-Mouse H-2Dd Monoclonal Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Isotype/Clone:
Isotype: Mouse IgG2a / Clone 34-5-8S
Applications:
Immunohistochemistry (IHC)
Testing Data
(Antibody)
Anti-Mouse H-2Dd (private; H-2.m4), PE (Clone 34-5-8S) (mouse IgG2a)
Other Names:
H-2Dd (private; H-2.m4); PE (Clone 34-5-8S) (mouse IgG2a); PE Anti-Mouse H-2Dd Monoclonal Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Isotype/Clone:
Isotype: Mouse IgG2a / Clone 34-5-8S
Applications:
Immunohistochemistry (IHC)
Testing Data
Anti-Mouse H-2Dd (private; H-2.m4), Purified (Clone 34-5-8S) (mouse IgG2a)
Other Names:
H-2Dd (private; H-2.m4); Purified (Clone 34-5-8S) (mouse IgG2a); Purified Anti Mouse H-2Dd Monoclonal Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Isotype/Clone:
Isotype: Mouse IgG2a/K / Clone 34-5-8S
Applications:
Immunohistochemistry (IHC), Cytotoxic Assay (CA), Flow Cytometry (FC/FACS)
Testing Data
Anti-Mouse H-2Dk-UNLB Antibody
Gene Names:
H-2Dk
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Mouse H-2Dk-FITC Conjugate Antibody
Gene Names:
H-2Dk
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Mouse H-2Dk-Biotin Conjugate Antibody
Gene Names:
H-2Dk
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
Anti-Mouse H-2Dk-R-PE Conjugate Antibody
Gene Names:
H-2Dk
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity Purified
Isotype/Clone:
Isotype: IgG2a kappa
Applications:
Flow Cytometry (FC/FACS)
 
H-2Kb antibody
Gene Names:
H-2Kb
Other Names:
Monoclonal H-2Kb; Anti-H-2Kb; H-Kb-2; H-Kb 2; MHC Class I H-2Kb
Host/Reactivities:
Host: Mouse
Isotype/Clone:
Isotype: IgG2a kappa / Clone AF6-88.5
Applications:
FC, IP, and IHC
 
(Antibody)
Popular Item
Anti-Mouse H-2KbDb (private), Biotin (Clone 5041.16.1) (mouse IgG2a)
Other Names:
H-2KbDb (private); Biotin (Clone 5041.16.1) (mouse IgG2a); Biotin Anti-Mouse H-2KbDb Monoclonal Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Isotype/Clone:
Isotype: Mouse IgG2a / Clone 5041.16.1
Applications:
Immunohistochemistry (IHC)
Testing Data
(Antibody)
Anti-Mouse H-2KbDb (private), FITC (Clone 5041.16.1) (mouse IgG2a)
Other Names:
H-2KbDb (private); FITC (Clone 5041.16.1) (mouse IgG2a); FITC Anti-Mouse H-2KbDb Monoclonal Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Isotype/Clone:
Isotype: Mouse IgG2a / Clone 5041.16.1
Applications:
Immunohistochemistry (IHC)
Testing Data
(Antibody)
Anti-Mouse H-2KbDb (private), PE (Clone 5041.16.1) (mouse IgG2a)
Other Names:
H-2KbDb (private); PE (Clone 5041.16.1) (mouse IgG2a); PE Anti-Mouse H-2KbDb Monoclonal Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Isotype/Clone:
Isotype: Mouse IgG2a / Clone 5041.16.1
Applications:
Immunohistochemistry (IHC)
Testing Data
Anti-Mouse H-2KbDb (private), Purified (Clone 5041.16.1) (mouse IgG2a)
Other Names:
H-2KbDb (private); Purified (Clone 5041.16.1) (mouse IgG2a); Purified Anti-Mouse H-2KbDb Monoclonal Antibody
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Purified from ascitic fluid via protein G affinity chromatography.
Isotype/Clone:
Isotype: Mouse IgG2a / Clone 5041.16.1
Applications:
Immunohistochemistry (IHC), Cytotoxic Assay (CA)
  • Testing Data
  • Testing Data (TD)
    Anti-Mouse H-2Kd (private; H-2.m31), Purified (Clone 31-3-4S) (mouse IgM)
    Other Names:
    H-2Kd (private; H-2.m31); Purified (Clone 31-3-4S) (mouse IgM); Purified Anti-Mouse H-2Kd Monoclonal Antibody
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgM / Clone 31-3-4S
    Applications:
    Immunohistochemistry (IHC)
     
    Anti-Mouse H-2Kd/H-2Dd-UNLB Antibody
    Gene Names:
    H-2Kd/H-2Dd; H-2Kd-H-2Dd
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    Affinity Purified
    Isotype/Clone:
    Isotype: IgG2a kappa
    Applications:
    Flow Cytometry (FC/FACS)
     
    Anti-Mouse H-2Kd/H-2Dd-FITC Conjugate Antibody
    Gene Names:
    H-2Kd/H-2Dd; H-2Kd-H-2Dd
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    Affinity Purified
    Isotype/Clone:
    Isotype: IgG2a kappa
    Applications:
    Flow Cytometry (FC/FACS)
     
    Anti-Mouse H-2Kd/H-2Dd-Biotin Conjugate Antibody
    Gene Names:
    H-2Kd/H-2Dd; H-2Kd-H-2Dd
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    Affinity Purified
    Isotype/Clone:
    Isotype: IgG2a kappa
    Applications:
    Flow Cytometry (FC/FACS)
     
    Anti-Mouse H-2Kd/H-2Dd-R-PE Conjugate Antibody
    Gene Names:
    H-2Kd/H-2Dd; H-2Kd-H-2Dd
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    Affinity Purified
    Isotype/Clone:
    Isotype: IgG2a kappa
    Applications:
    Flow Cytometry (FC/FACS)
     
    Anti-Mouse H-2KdDd, Biotin (Clone 34-1-2S) (mouse IgG2a)
    Gene Names:
    H-2KdDd
    Other Names:
    Biotin (Clone 34-1-2S) (mouse IgG2a); Biotin Anti-Mouse H-2KdDd Monoclonal Antibody
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgG2a / Clone 34-1-2S
    Applications:
    Immunohistochemistry (IHC)
    Testing Data
    (Antibody)
    Anti-Mouse H-2KdDd, FITC (Clone 34-1-2S) (mouse IgG2a)
    Gene Names:
    H-2KdDd
    Other Names:
    FITC (Clone 34-1-2S) (mouse IgG2a); FITC Anti-Mouse H-2KdDd Monoclonal Antibody
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgG2a / Clone 34-1-2S
    Applications:
    Immunohistochemistry (IHC)
    Testing Data
    (Antibody)
    Anti-Mouse H-2KdDd, PE (Clone 34-1-2S) (mouse IgG2a)
    Gene Names:
    H-2KdDd
    Other Names:
    PE (Clone 34-1-2S) (mouse IgG2a); PE Anti-Mouse H-2KdDd Monoclonal Antibody
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgG2a / Clone 34-1-2S
    Applications:
    Immunohistochemistry (IHC)
    Testing Data
    Anti-Mouse H-2KdDd, Affinity Purified (Clone 34-1-2S) (mouse IgG2a)
    Gene Names:
    H-2KdDd
    Other Names:
    Affinity Purified (Clone 34-1-2S) (mouse IgG2a)
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgG2a / Clone 34-1-2S
    Applications:
    Immunohistochemistry (IHC), Cytotoxic Assay (CA)
     
    Anti-Mouse H-2KdDd (public), Purified, (Clone 34-7-23S) (mouse IgM)
    Gene Names:
    H-2KdDd
    Other Names:
    H-2KdDd (public); Purified; (Clone 34-7-23S) (mouse IgM)
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgM / Clone 34-7-23S
    Applications:
    Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
     
    Anti-Mouse H-2Kk, Biotin (Clone H100-5-28) (mouse IgG2a)
    Gene Names:
    H-2Kk
    Other Names:
    Biotin (Clone H100-5-28) (mouse IgG2a); Anti-Mouse H-2Kk Monoclonal Antibody
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgG2a / Clone H100-5-28
    Applications:
    Immunohistochemistry (IHC)
    Testing Data
    (Antibody)
    Anti-Mouse H-2Kk, PE (Clone H100-5-28) (mouse IgG2a)
    Gene Names:
    H-2Kk
    Other Names:
    PE (Clone H100-5-28) (mouse IgG2a); Anti-Mouse H-2Kk Monoclonal Antibody
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgG2a / Clone H100-5-28
    Applications:
    Immunohistochemistry (IHC)
    Testing Data
    Anti-Mouse H-2Kk, FITC (Clone H100-5-28) (mouse IgG2a)
    Gene Names:
    H-2Kk
    Other Names:
    FITC (Clone H100-5-28) (mouse IgG2a); Anti-Mouse H-2Kk Monoclonal Antibody
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgG2a / Clone H100-5-28
    Applications:
    Immunohistochemistry (IHC)
    Testing Data
    (Antibody)
    Anti-Mouse H-2Kk, Affinity Purfied (Clone H100-5-28) (mouse IgG2a)
    Gene Names:
    H-2Kk
    Other Names:
    Affinity Purfied (Clone H100-5-28) (mouse IgG2a); Anti-Mouse H-2Kk Monoclonal Antibody
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgG2a / Clone H100-5-28
    Applications:
    Immunohistochemistry (IHC)
    Testing Data
    Anti-Mouse H-2Ld (public; H-2.m65), Biotin (Clone 30-5-7S) (mouse IgG2a)
    Other Names:
    H-2Ld (public; H-2.m65); Biotin (Clone 30-5-7S) (mouse IgG2a); Anti-H-2Ld Monoclonal Antibody
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgG2a / Clone 30-5-7S
    Testing Data
    (Antibody)
    Anti-Mouse H-2Ld (public; H-2.m65), FITC (Clone 30-5-7S) (mouse IgG2a)
    Other Names:
    H-2Ld (public; H-2.m65); FITC (Clone 30-5-7S) (mouse IgG2a); Anti-H-2Ld Monoclonal Antibody
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse IgG2a / Clone 30-5-7S
    Applications:
    Cytotoxic Assay (CA), Immunohistochemistry (IHC)
    Testing Data
    (Antibody)
    Popular Item
    Displaying 1 to 50 (of 4828 products)
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