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Monoclonal Antibody

What is Monoclonal Antibody Purification?

Monoclonal antibody purification involves collecting the media from the cultured hybridomas, centrifuging and filtering the media, and then using chromatographic or physiochemical fractionation methods to isolate the antibodies. Physiochemical fractionation involves using differential precipitation, size-exclusion or solid-phase binding of proteins based on size, charge or other chemical characteristics. These techniques include Size Exclusion Chromotography (SEC), ammonium sulfate precipitation and Ion Exchange Chromotography. Antibodies can also be purified based on class-specific affinity which involves using immobilized agents, such as proteins or lectins, that have an affinity for the isotype of the antibody of interest. Antigen-affinity chromatography can also be used to purify antibodies, but this method is not commonly used for monoclonal antibodies since the target antibody is generally the only immunoglobulin in the sample.

Displaying 1 to 50 (of 4503 products) order by Products Name.
anti-M human blood antigen IgM
Other Names:
Anti-M blood antigen antibody; anti-M IgM (murine monoclonal)
Host/Reactivities:
Host: Mouse hybridoma
Purification:
Protein A purified.
 
mAb anti-human M-CSF
Gene Names:
M-CSF
Other Names:
Macrophage Colony Stimulating Factor (M-CSF); Mouse monoclonal antibody to human Macrophage Colony Stimulating Factor (M-CSF); macrophage colony-stimulating factor; M-CSF {N-terminal}; partial
Host/Reactivities:
Host: Mouse / Reactivity: Human. Other species not testedDoes not cross react with BSA or other human cytokines tested suc...
Purification:
Protein G affinity purified
Isotype/Clone:
Isotype: IgG2b,k / Clone 116
Applications:
ELISA (EIA)
 
mAb anti-human M-CSF
Gene Names:
M-CSF
Other Names:
Macrophage Colony Stimulating Factor (M-CSF); Mouse monoclonal antibody to human Macrophage Colony Stimulating Factor (M-CSF); macrophage colony-stimulating factor; M-CSF {N-terminal}; partial
Host/Reactivities:
Host: Mouse / Reactivity: Human. Other species not testedDoes not cross react with BSA or other human cytokines tested suc...
Purification:
Protein G affinity purified
Isotype/Clone:
Isotype: IgG2a,k / Clone 21
Applications:
ELISA (EIA)
 
M-CSF, Mouse, mAb ER-MP58
Gene Names:
M-CSF
Other Names:
macrophage colony-stimulating factor; M-CSF {N-terminal}; partial
Host/Reactivities:
Host: Rat / Reactivity: Mouse
Isotype/Clone:
Isotype: Rat IgG2a
Applications:
F, Flow Cytometry (FC/FACS)
 
mAb anti-human M-CSF
Gene Names:
M-CSF
Other Names:
Macrophage Colony Stimulating Factor (M-CSF); Mouse monoclonal antibody to human Macrophage Colony Stimulating Factor (M-CSF); macrophage colony-stimulating factor; M-CSF {N-terminal}; partial
Host/Reactivities:
Host: Mouse / Reactivity: Human. Other species not testedDoes not cross react with BSA or other human cytokines tested suc...
Purification:
Protein G affinity purified
Isotype/Clone:
Isotype: IgG2a,k / Clone 692
Applications:
ELISA (EIA)
 
Rat Anti-Mouse M-CSF
Gene Names:
M-CSF; Csf1; op; Csfm; MCSF; C87615
Other Names:
macrophage colony-stimulating factor 1 isoform 2; colony stimulating factor 1 (macrophage)
Host/Reactivities:
Host: Rat / Reactivity: Mouse
Isotype/Clone:
Isotype: IgG2 / Clone 14W13
Applications:
Western Blot (WB), Neutralization
 
Mouse Anti-Human M-CSF
Gene Names:
M-CSF; CSF1; MCSF; CSF-1
Other Names:
macrophage colony-stimulating factor 1 isoform a; colony stimulating factor 1 (macrophage)
Host/Reactivities:
Host: Mouse / Reactivity: Human
Isotype/Clone:
Isotype: IgG2 / Clone (3V17)
Applications:
Western Blot (WB), Neutr
 
Rat Anti-Mouse M-CSF R
Gene Names:
M-CSF R; Csf1r; Fms; Fim2; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; M-CSF-R; AI323359
Other Names:
CSF1; MCSF; CSF-1; Macrophage colony-stimulating factor 1 receptor; colony stimulating factor 1 receptor
Host/Reactivities:
Host: Rat / Reactivity: Mouse
Isotype/Clone:
Isotype: IgG2 / Clone 7M23
Applications:
Western Blot (WB), Flow Cytometry (FC/FACS)
 
Mouse Anti-Human M-CSF R
Gene Names:
M-CSF R; CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Other Names:
CSF1; MCSF; CSF-1; macrophage colony-stimulating factor 1 receptor; colony stimulating factor 1 receptor
Host/Reactivities:
Host: Mouse / Reactivity: Human
Isotype/Clone:
Isotype: IgG1 / Clone (5V36)
Applications:
Western Blot (WB), Neutr
 
M-Ficolin antibody
Other Names:
Monoclonal M-Ficolin; Anti-M-Ficolin; Collagen/fibrinogen domain-containing protein 1; FCN1; Ficolin M; FCN-1; FCNM; M-FCN; Ficolin-1; Ficolin-A; Ficolin-alpha; M-ficolin; Ficolin 1
Host/Reactivities:
Host: Mouse / Reactivity: Human
Purification:
M-Ficolin antibody was purified from cell culture supernatant by protein A/G chromatography
Isotype/Clone:
Isotype: IgG1 kappa / Clone M81381
Applications:
ELISA (EIA), Immunohistochemistry (IHC)
  • Immunohistochemistry (IHC)
  • Immunohistochemistry (IHC)
    M-Ficolin antibody
    Other Names:
    Monoclonal M-Ficolin; Anti-M-Ficolin; FCN1; Ficolin 1; Ficolin M; FCN-1; FCNM; M-FCN; Collagen/fibrinogen domain-containing protein 1; M-Ficolin; Ficolin-1; Ficolin-A; Ficolin-alpha
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Purification:
    M-Ficolin antibody was purified from cell culture supernatant by protein A/G chromatography
    Isotype/Clone:
    Isotype: IgG1 kappa / Clone M81530
    Applications:
    ELISA (EIA)
     
    Mycobacterium tuberculosis
    Isotype/Clone:
    Isotype: IgG1
     
    Mycobacterium tuberculosis
    Purification:
    Purified
    Isotype/Clone:
    Isotype: IgG1
    Applications:
    IFA, ELISA
     
    Mycobacterium tuberculosis
    Isotype/Clone:
    Isotype: IgG2b
     
    Mycobacterium tuberculosis
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a
     
    Mycobacterium tuberculosis
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a
     
    Mycobacterium tuberculosis
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a
     
    Mycobacterium tuberculosis
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a
     
    M13 + fd + F1 Filamentous Phages antibody (FITC)
    Gene Names:
    m13
    Other Names:
    Monoclonal M13 Phage; Anti-M13 Phage; M + fd + F1 Filamentous Phages-13; M + fd + F1 Filamentous Phages 13; M13 + fd + F1 Filamentous Phages
    Host/Reactivities:
    Host: Mouse / Reactivity: B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of g8p AEGD...
    Purification:
    Protein A affinity chromatography
    Isotype/Clone:
    Isotype: IgG2b / Clone B62-FE2
    Applications:
    User optimized
     
    M13 + fd + F1 Filamentous Phages antibody (biotin)
    Other Names:
    Monoclonal M13 Phage; Anti-M13 Phage; M + fd + F1 Filamentous Phages-13; M13 + fd + F1 Filamentous Phages; M + fd + F1 Filamentous Phages 13
    Host/Reactivities:
    Host: Mouse / Reactivity: This antibody recognizes an epitope on pVIII(phage coat protein) covering the N-terminal region of g...
    Purification:
    Protein A affinity chromatography
    Isotype/Clone:
    Isotype: IgG2b / Clone B62-FE2
    Applications:
    ELISA (EIA), Western Blot (WB)
     
    M13/fd/F1 FP mAb(HRP)
    Other Names:
    Monoclonal M13 + fd + F1 Filamentous Phages; Anti-M13 + fd + F1 Filamentous Phages; M13 + fd + F1 Filamentous Phages; M + fd + F1 Filamentous Phages 13; M13/fd/F1 Filamentous Phages; M + fd + F1 Filam...
    Host/Reactivities:
    Host: Mouse / Reactivity: B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p ...
    Purification:
    Protein A affinity chromatography
    Isotype/Clone:
    Isotype: IgG2b / Clone B62-FE2
    Applications:
    ELISA (EIA)
     
    M13 + fd + F1 Filamentous Phages antibody
    Other Names:
    Monoclonal M13 + fd + F1 Filamentous Phages; Anti-M13 Phage; M + fd + F1 Filamentous Phages 13; M + fd + F1 Filamentous Phages-13; M13 + fd + F1 Filamentous Phages
    Host/Reactivities:
    Host: Mouse / Reactivity: Clone B62-FE2 binds to an internal epitope on the N-terminus of pVIII
    Purification:
    Protein A affinity chromatography
    Isotype/Clone:
    Isotype: IgG2b / Clone B62-FE2
    Applications:
    ELISA (EIA), Phage Display, Immunoelectron microscopy, Western Blot (WB)
     
    M13 + fd + F1 Filamentous Phages antibody
    Other Names:
    Monoclonal M13 + fd + F1 Filamentous Phages; Anti-M13 + fd + F1 Filamentous Phages; M + fd + F1 Filamentous Phages 13; M13 + fd + F1 Filamentous Phages; M + fd + F1 Filamentous Phages-13; M13/fd/F1 Fi...
    Host/Reactivities:
    Host: Mouse / Reactivity: This antibody recognizes an epitope on pVIII (phage coat protein) covering the N-Terminal region of ...
    Purification:
    Protein A affinity chromatography
    Isotype/Clone:
    Isotype: IgG2b / Clone B62-FE2
    Applications:
    ELISA (EIA), Phage Display, Immunofluoresence (IF), Western Blot (WB)
     
    M13 Bacteriophage (Biotin)
    Other Names:
    Anti -M13 Bacteriophage (Biotin)
    Host/Reactivities:
    Host: Mouse
    Purification:
    PurifiedPurified
    Isotype/Clone:
    Isotype: IgG2a / Clone 3H2610
    Applications:
    ELISA (EL/EIA), Flow Cytometry (FC/FACS)
     
    MOUSE ANTI M13 BACTERIOPHAGE
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a / Clone E1
    Applications:
    Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS)
    Testing Data
    Mouse Anti-M13 Bacteriophage (FITC)
    Host/Reactivities:
    Host: Mouse
    Purification:
    Purified
    Isotype/Clone:
    Isotype: IgG2a / Clone 3H2610
    Applications:
    FLISA, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
    Flow Cytometry (FC/FACS)
    MOUSE ANTI M13 BACTERIOPHAGE:Biotin
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a / Clone E1
    Applications:
    ELISA (EIA), Flow cytometry (FC/FACS)
    Testing Data
    M13 Bacteriophage
    Other Names:
    Anti -M13 Bacteriophage
    Host/Reactivities:
    Host: Mouse
    Purification:
    PurifiedPurified
    Isotype/Clone:
    Isotype: IgG2a / Clone 10F25
    Applications:
    ELISA (EL/EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
     
    M13 bacteriophage (g3p)
    Gene Names:
    III; G3P
    Other Names:
    Coat protein A; Minor coat protein; structural protein; Gene 3 protein
    Host/Reactivities:
    Host: Mouse
    Purification:
    Protein A/G Chromatography
    Isotype/Clone:
    Isotype: IgG2a / Clone E1
    Applications:
    ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
     
    M13 bacteriophage (gp3) Biotinylated
    Gene Names:
    III; G3P
    Other Names:
    Coat protein A; Minor coat protein; structural protein; Gene 3 protein
    Host/Reactivities:
    Host: Mouse
    Purification:
    Protein A/G Chromatography
    Isotype/Clone:
    Isotype: IgG2a / Clone E1
    Applications:
    ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
    Testing Data
    M13 Bacteriophage (gp8)
    Gene Names:
    VIII
    Other Names:
    structural protein
    Host/Reactivities:
    Host: Mouse / Reactivity: Ubiquitous
    Isotype/Clone:
    Isotype: IgG2a / Clone RL-ph2
    Applications:
    Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC) Frozen, Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
    Western Blot/Blotting
    M13 phage antibody
    Other Names:
    Monoclonal M13 phage; Anti-M13 phage; M13p06; M13 phage; M13 phage coat protein g8p
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a / Clone RL-ph2
    Applications:
    Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
     
    M13 phage antibody
    Other Names:
    Monoclonal M13 phage; Anti-M13 phage; M13p06; M13 phage; M13 phage coat protein g8p
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2b / Clone RL-ph1
    Applications:
    Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
     
    Mouse anti M13 phage coat protein g8p
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2b / Clone RL-ph1
    Applications:
    Affinity chromatography, Flow Cytometry (FC), Immunocytochemistry (IHC), Western Blot (WB),Elisa (EIA), Immunoblotting
     
    Mouse anti M13 phage coat protein g8p
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a / Clone RL-ph2
    Applications:
    Affinity chromatography, Flow Cytometry (FC), Immunocytochemistry (IHC), Western Blot (WB)
    Testing Data
    M13, fd, F1 Bacteriophages, Filamentous (FITC)
    Other Names:
    Anti -M13; fd; F1 Bacteriophages; Filamentous (FITC)
    Host/Reactivities:
    Host: Mouse / Reactivity: E Coli
    Purification:
    Affinity PurifiedPurified by Protein A affinity chromatography.
    Isotype/Clone:
    Isotype: IgG2b / Clone 5F167
    Applications:
    ELISA (EL/EIA)
     
    M13, fd, F1 Bacteriophages, Filamentous
    Other Names:
    Anti -M13; fd; F1 Bacteriophages; Filamentous
    Host/Reactivities:
    Host: Mouse
    Purification:
    Affinity PurifiedPurified by Protein A affinity chromatography.
    Isotype/Clone:
    Isotype: IgG2b / Clone 5F167
    Applications:
    ELISA (EL/EIA), Western Blot (WB)
     
    M13, fd, F1 Bacteriophages, Filamentous (Biotin)
    Other Names:
    Anti -M13; fd; F1 Bacteriophages; Filamentous (Biotin)
    Host/Reactivities:
    Host: Mouse / Reactivity: E Coli
    Purification:
    Affinity PurifiedPurified by Protein A affinity chromatography.
    Isotype/Clone:
    Isotype: IgG2b / Clone 5F167
    Applications:
    ELISA (EL/EIA), Western Blot (WB)
     
    M13, fd, F1 Bacteriophages, Filamentous (Biotin)
    Other Names:
    Anti -M13; fd; F1 Bacteriophages; Filamentous (Biotin)
    Host/Reactivities:
    Host: Mouse / Reactivity: E Coli
    Purification:
    Affinity PurifiedPurified by Protein A affinity chromatography.
    Isotype/Clone:
    Isotype: IgG2b / Clone 9L390
    Applications:
    ELISA (EL/EIA), Western Blot (WB)
     
    M13, fd, F1 Bacteriophages, Filamentous
    Other Names:
    Anti -M13; fd; F1 Bacteriophages; Filamentous
    Host/Reactivities:
    Host: Mouse
    Purification:
    SupernatantHybridoma culture supernatant
    Isotype/Clone:
    Isotype: IgG2b / Clone 5F167
    Applications:
    ELISA (EL/EIA), Western Blot (WB)
     
    M13, fd, F1 Bacteriophages, Filamentous (HRP)
    Other Names:
    Anti -M13; fd; F1 Bacteriophages; Filamentous (HRP)
    Host/Reactivities:
    Host: Mouse / Reactivity: E Coli
    Purification:
    Affinity PurifiedPurified by Protein A affinity chromatography.
    Isotype/Clone:
    Isotype: IgG2b / Clone 5F167
    Applications:
    ELISA (EL/EIA)
     
    M13, fd, F1 Bacteriophages, Filamentous
    Other Names:
    Anti -M13; fd; F1 Bacteriophages; Filamentous
    Host/Reactivities:
    Host: Mouse / Reactivity: E Coli
    Purification:
    Affinity PurifiedPurified by Protein A affinity chromatography.
    Isotype/Clone:
    Isotype: IgG2b / Clone 5F167
    Applications:
    ELISA (EL/EIA), Western Blot (WB)
     
    M13/fd/F1 Filamentous Phages antibody (Biotin)
    Other Names:
    Monoclonal M13/fd/F1; Anti M13/fd/F1; M/fd/F1 13; M13/fd/F1; M/fd/F1-13
    Host/Reactivities:
    Host: Mouse / Reactivity: To be determined by the end user
    Purification:
    M13/fd/F1 Filamentous Phages antibody (Biotin) was purified by Protein A affinity chromatography
    Isotype/Clone:
    Isotype: IgG2b / Clone B62-FE2
    Applications:
    ELISA (EIA), Western Blot (WB)
     
    M13/fd/F1 Filamentous Phages antibody (Biotin)
    Other Names:
    Monoclonal M13/fd/F1; Anti M13/fd/F1; M/fd/F1-13; M13/fd/F1; M/fd/F1 13
    Host/Reactivities:
    Host: Mouse / Reactivity: To be determined by the end user
    Isotype/Clone:
    Isotype: IgG2b / Clone B62-FE2
    Applications:
    ELISA (EIA), Western Blot (WB)
     
    MAb to Filamentous Phages M13/fd/F1
    Other Names:
    Monoclonal Antibody to Filamentous Phages; M13; fd; F1
    Host/Reactivities:
    Host: Host: MouseSource: Tissue Culture
    Purification:
    Protein A chromatography
    Isotype/Clone:
    Isotype: IgG2b / Clone B62-FE2
    Applications:
    EIA/ELISA, Western Blot
     
    RAT ANTI M31/HP1
    Gene Names:
    M31; Cbx1; Cbx; HP1B; MOD1; Cbx-rs2; Hp1beta; E430007M08Rik
    Other Names:
    chromobox protein homolog 1; chromobox 1
    Host/Reactivities:
    Host: Rat / Reactivity: Mouse
    Isotype/Clone:
    Isotype: IgG2b / Clone MAC353
    Applications:
    Immunofluorescence (IF), ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB), Immunohistology - Frozen (IHC), Chromatin Immunoprecipitation (IP) (ChIP)
     
    Anti-M6PR Rabbit Monoclonal Antibody
    Gene Names:
    M6PR; IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R; MPR300; CI-M6PR; MPR 300; M6P/IGF2R
    Other Names:
    Cation-independent mannose-6-phosphate receptor; CI Man-6-P receptor; CI-MPR; 300 kDa mannose 6-phosphate receptor; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; IGF-I...
    Host/Reactivities:
    Host: Rabbit / Reactivity: Human, Mouse, Rat
    Purification:
    Affinity-chromatography
    Applications:
    Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
    • Immunohistochemistry (IHC)
    • Western Blot (WB)
      M6PR antibody
      Gene Names:
      M6PR; SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR
      Other Names:
      Cation-dependent mannose-6-phosphate receptor isoform 1; mannose-6-phosphate receptor; cation dependent
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      By protein-A affinity chromatography
      Isotype/Clone:
      Isotype: IgG1,k / Clone AT2B4
      Applications:
      ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
      • Western Blot (WB)
      • Immunofluorescence (IF)
        M6PR
        Gene Names:
        M6PR; IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R; MPR300; CI-M6PR; MPR 300; M6P/IGF2R
        Other Names:
        CI Man-6-P receptor; CI-MPR; IGF-II receptor; Cation-independent mannose-6-phosphate receptor; 300 kDa mannose 6-phosphate receptor; Insulin-like growth factor 2 receptor; Insulin-like growth factor I...
        Host/Reactivities:
        Host: Rabbit / Reactivity: Human, Mouse, Rat
        Purification:
        ProA affinity purified
        Isotype/Clone:
        Isotype: IgG
        Applications:
        WB, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
         
        M6PR antibody
        Gene Names:
        M6PR; SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR
        Other Names:
        Mouse monoclonal M6PR antibody; Monoclonal M6PR antibody; Anti M6PR antibody; Cation-dependent mannose-6-phosphate receptor isoform 1 antibody; CD-MPR antibody; MPR 46 antibody; MPR-46 antibody; MPR46...
        Host/Reactivities:
        Host: Mouse / Reactivity: Human
        Purification:
        Purified by Protein A chromatography
        Isotype/Clone:
        Isotype: IgG1, kappa / Clone AT2B4
        Applications:
        ELISA (EIA), Western Blot (WB)
        Testing Data
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