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Monoclonal Antibody

What is Monoclonal Antibody Purification?

Monoclonal antibody purification involves collecting the media from the cultured hybridomas, centrifuging and filtering the media, and then using chromatographic or physiochemical fractionation methods to isolate the antibodies. Physiochemical fractionation involves using differential precipitation, size-exclusion or solid-phase binding of proteins based on size, charge or other chemical characteristics. These techniques include Size Exclusion Chromotography (SEC), ammonium sulfate precipitation and Ion Exchange Chromotography. Antibodies can also be purified based on class-specific affinity which involves using immobilized agents, such as proteins or lectins, that have an affinity for the isotype of the antibody of interest. Antigen-affinity chromatography can also be used to purify antibodies, but this method is not commonly used for monoclonal antibodies since the target antibody is generally the only immunoglobulin in the sample.

Displaying 1 to 31 (of 31 products) order by Products Name.
Qa-1b (H2 T23, H2 Class I Histocompatibility Antigen D37 alpha Chain, H2 Q1, Histocompatibility 2 T Region Locus 23, MHC Class I Antigen Qa1, MHC ClassIb Qa1, OTTMUSP00000015895, Qa1)
Other Names:
Anti -Qa-1b (H2 T23; H2 Class I Histocompatibility Antigen D37 alpha Chain; H2 Q1; Histocompatibility 2 T Region Locus 23; MHC Class I Antigen Qa1; MHC ClassIb Qa1; OTTMUSP00000015895; Qa1)
Host/Reactivities:
Host: Mouse / Reactivity: Mouse
Purification:
Affinity PurifiedPurified by Protein G affinity chromatography.
Isotype/Clone:
Isotype: IgG1,k / Clone 11E331
Applications:
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Neutralization
  • Western Blot
  • Flow Cytometry
  • Flow Cytometry
    Qa-2 (PE)
    Gene Names:
    H2-Q8; Qa8; Qa-2; Qa-8; H-2Q8; Ms10t; MMS10-T
    Other Names:
    Anti -Qa-2 (PE); Qa-2 cell surface antigen; histocompatibility 2; Q region locus 8
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    PurifiedPurified
    Isotype/Clone:
    Isotype: IgG2a,k / Clone 5K44
    Applications:
    Flow Cytometry (FC/FACS)
     
    Qa-2 (FITC)
    Gene Names:
    H2-Q8; Qa8; Qa-2; Qa-8; H-2Q8; Ms10t; MMS10-T
    Other Names:
    Anti -Qa-2 (FITC); Qa-2 cell surface antigen; histocompatibility 2; Q region locus 8
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    PurifiedPurified
    Isotype/Clone:
    Isotype: IgG2a,k / Clone 5K44
    Applications:
    Flow Cytometry (FC/FACS)
     
    Qa-2
    Gene Names:
    H2-Q8; Qa8; Qa-2; Qa-8; H-2Q8; Ms10t; MMS10-T
    Other Names:
    Anti -Qa-2; Qa-2 cell surface antigen; histocompatibility 2; Q region locus 8
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    PurifiedPurified
    Isotype/Clone:
    Isotype: IgG2a,k / Clone 5K44
    Applications:
    Flow Cytometry (FC/FACS)
     
    Mouse Anti-Mouse Qa-2-UNLB
    Other Names:
    Mouse Anti-Mouse Qa-2 - Purified; Unlabeled Antibody; Mouse Anti-Mouse Qa-2
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse (C3H/KBR) IgG2a kappa / Clone 695H1-9-9
    Applications:
    Flow Cytometry
     
    Mouse Anti-Mouse Qa-2-FITC
    Other Names:
    Mouse Anti-Mouse Qa-2 - FITC; Mouse Anti-Mouse Qa-2
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse (C3H/KBR) IgG2a kappa / Clone 695H1-9-9
    Applications:
    Flow Cytometry
     
    Mouse Anti-Mouse Qa-2-BIOT
    Other Names:
    Mouse Anti-Mouse Qa-2 - Biotin; Mouse Anti-Mouse Qa-2
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse (C3H/KBR) IgG2a kappa / Clone 695H1-9-9
    Applications:
    Flow Cytometry
     
    Mouse Anti-Mouse Qa-2-PE
    Other Names:
    Mouse Anti-Mouse Qa-2 - Phycoerythrin-Labeled; Mouse Anti-Mouse Qa-2
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Isotype/Clone:
    Isotype: Mouse (C3H/KBR) IgG2a kappa / Clone 695H1-9-9
    Applications:
    Flow Cytometry
     
    Anti-Mouse Qa-2-UNLB Antibody
    Gene Names:
    Qa-2
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    Affinity Purified
    Isotype/Clone:
    Isotype: IgG2a kappa
    Applications:
    Flow Cytometry (FC/FACS)
     
    Anti-Mouse Qa-2-FITC Conjugate Antibody
    Gene Names:
    Qa-2
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    Affinity Purified
    Isotype/Clone:
    Isotype: IgG2a kappa
    Applications:
    Flow Cytometry (FC/FACS)
     
    Anti-Mouse Qa-2-Biotin Conjugate Antibody
    Gene Names:
    Qa-2
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    Affinity Purified
    Isotype/Clone:
    Isotype: IgG2a kappa
    Applications:
    Flow Cytometry (FC/FACS)
     
    Anti-Mouse Qa-2-R-PE Conjugate Antibody
    Gene Names:
    Qa-2
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    Affinity Purified
    Isotype/Clone:
    Isotype: IgG2a kappa
    Applications:
    Flow Cytometry (FC/FACS)
     
    Qa-2 (Biotin)
    Gene Names:
    H2-Q8; Qa8; Qa-2; Qa-8; H-2Q8; Ms10t; MMS10-T
    Other Names:
    Anti -Qa-2 (Biotin); Qa-2 cell surface antigen; histocompatibility 2; Q region locus 8
    Host/Reactivities:
    Host: Mouse / Reactivity: Mouse
    Purification:
    PurifiedPurified
    Isotype/Clone:
    Isotype: IgG2a,k / Clone 5K44
    Applications:
    Flow Cytometry (FC/FACS)
     
    Qa2 antibody (FITC)
    Gene Names:
    Qa2; H2-Q7; Q9; Ped; Qa7; Qa-2; Qa-7; H-2Q7
    Other Names:
    Monoclonal Qa2; Anti-Qa2; Qa 2; Qa2 antigen; histocompatibility 2; Q region locus 7
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a kappa / Clone 69H1-9-9
    Applications:
    Flow Cytometry (FC/FACS)
    Flow Cytometry (FC/FACS)
    QARS (Glutamine--tRNA Ligase, Glutaminyl-tRNA Synthetase, GlnRS)
    Gene Names:
    QARS; GLNRS; PRO2195
    Other Names:
    Anti -QARS (Glutamine--tRNA Ligase; Glutaminyl-tRNA Synthetase; GlnRS); QARS protein; partial
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Purification:
    Affinity PurifiedPurified by Protein A affinity chromatography.
    Isotype/Clone:
    Isotype: IgG2b,k / Clone 5F5
    Applications:
    ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
     
    QPCT (Glutaminyl-peptide Cyclotransferase, Glutaminyl Cyclase, Glutaminyl-tRNA Cyclotransferase, Glutamyl Cyclase)
    Gene Names:
    Qpct; 5730422A13Rik
    Other Names:
    Anti -QPCT (Glutaminyl-peptide Cyclotransferase; Glutaminyl Cyclase; Glutaminyl-tRNA Cyclotransferase; Glutamyl Cyclase); Qpct protein; glutaminyl-peptide cyclotransferase (glutaminyl cyclase)
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Purification:
    Affinity PurifiedPurified by Protein A affinity chromatography.
    Isotype/Clone:
    Isotype: IgG2a,k / Clone 4E11
    Applications:
    ELISA (EL/EIA), Western Blot (WB)
     
    QPRT antibody
    Gene Names:
    QPRT; QPRTase; HEL-S-90n
    Other Names:
    Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; QPRTase;; nicotinate-nucleotide pyrophosphorylase
    Host/Reactivities:
    Host: Mouse / Reactivity: Human
    Purification:
    QPRT antibody was purified by affinity chromatography.
    Isotype/Clone:
    Isotype: IgG2a / Clone 3B3
    Applications:
    Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
    • Flow Cytometry (FC/FACS)
    • Western Blot (WB)
    • Immunofluorescence (IF)
      QPRT antibody
      Gene Names:
      QPRT; QPRTase; HEL-S-90n
      Other Names:
      Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; QPRTase;; nicotinate-nucleotide pyrophosphorylase
      Host/Reactivities:
      Host: Mouse / Reactivity: Human
      Purification:
      QPRT antibody was purified by affinity chromatography.
      Isotype/Clone:
      Isotype: IgG1 / Clone 7A8
      Applications:
      Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
      • Flow Cytometry (FC/FACS)
      • Western Blot (WB)
      • Immunohistochemistry (IHC)
        QPRT antibody
        Gene Names:
        QPRT; QPRTase; HEL-S-90n
        Other Names:
        Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; QPRTase;; nicotinate-nucleotide pyrophosphorylase
        Host/Reactivities:
        Host: Mouse / Reactivity: Human
        Purification:
        QPRT antibody was purified by affinity chromatography.
        Isotype/Clone:
        Isotype: IgG1 / Clone 4B4
        Applications:
        Flow Cytometry (FC/FACS), Western Blot (WB)
        • Flow Cytometry (FC/FACS)
        • Western Blot (WB)
          QPRT antibody
          Gene Names:
          QPRT; QPRTase; HEL-S-90n
          Other Names:
          Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; QPRTase;; nicotinate-nucleotide pyrophosphorylase
          Host/Reactivities:
          Host: Mouse / Reactivity: Human
          Purification:
          QPRT antibody was purified by affinity chromatography.
          Isotype/Clone:
          Isotype: IgG1 / Clone 1C10
          Applications:
          Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
          • Western Blot (WB)
          • Immunohistochemistry (IHC)
          • Immunofluorescence (IF)
            QPRT antibody
            Gene Names:
            QPRT; QPRTase; HEL-S-90n
            Other Names:
            Nicotinate nucleotide pyrophosphorylase (carboxylating); Nicotinate nucleotide pyrophosphorylase carboxylating; Quinolinate phosphoribosyltransferase; QAPRTase; nicotinate-nucleotide pyrophosphorylase
            Host/Reactivities:
            Host: Mouse / Reactivity: Human
            Purification:
            By protein-A affinity chromatography
            Isotype/Clone:
            Isotype: IgG2a, k / Clone AT24C4
            Applications:
            ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
            • Western Blot (WB)
            • Immunofluorescence (IF)
              QPRT antibody
              Gene Names:
              QPRT; QPRTase; HEL-S-90n
              Other Names:
              Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; QPRTase;; nicotinate-nucleotide pyrophosphorylase
              Host/Reactivities:
              Host: Mouse / Reactivity: Human
              Purification:
              QPRT antibody was purified by affinity chromatography.
              Isotype/Clone:
              Isotype: IgG2b / Clone 4 E5
              Applications:
              Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
              • Flow Cytometry (FC/FACS)
              • Western Blot (WB)
              • Immunofluorescence (IF)
                QPRT antibody
                Gene Names:
                QPRT; QPRTase; HEL-S-90n
                Other Names:
                Monoclonal QPRT; Anti-QPRT; Quinolinate phosphoribosyltransferase; QPRTase;; nicotinate-nucleotide pyrophosphorylase
                Host/Reactivities:
                Host: Mouse / Reactivity: Human
                Purification:
                QPRT antibody was purified by affinity chromatography.
                Isotype/Clone:
                Isotype: IgG1 / Clone 4B3
                Applications:
                Flow Cytometry (FC/FACS), Western Blot (WB)
                • Flow Cytometry (FC/FACS)
                • Western Blot (WB)
                  Quinidine Monoclonal Antibody
                  Other Names:
                  Anti-Quinidine; Quinidine
                  Host/Reactivities:
                  Host: Mouse
                  Isotype/Clone:
                  Isotype: IgG2b / Clone QD11-228.6.5
                  Applications:
                  These antibodies have been qualified for use in ELISA to detect quinidine.
                  Testing Data TD
                  Quinidine Monoclonal Antibody
                  Other Names:
                  Anti-Quinidine; Quinidine
                  Host/Reactivities:
                  Host: Mouse
                  Isotype/Clone:
                  Isotype: IgG1 / Clone QD11-261.3.6
                  Applications:
                  These antibodies have been qualified for use in ELISA to detect quinidine.
                  Testing Data TD
                  Quinidine Monoclonal Antibody
                  Other Names:
                  Anti-Quinidine; Quinidine
                  Host/Reactivities:
                  Host: Mouse
                  Isotype/Clone:
                  Isotype: IgG1 / Clone QD11-265.2.3
                  Applications:
                  These antibodies have not been qualified for use in ELISA to detect quinidine.
                  Testing Data TD
                  Quinidine Monoclonal Antibody
                  Other Names:
                  Anti-Quinidine; Quinidine
                  Host/Reactivities:
                  Host: Mouse
                  Isotype/Clone:
                  Isotype: IgG1 / Clone QD13-406.6.1
                  Applications:
                  These antibodies have been qualified for use in ELISA to detect quinidine.
                  Testing Data TD
                  Quinidine Monoclonal Antibody
                  Other Names:
                  Anti-Quinidine; Quinidine
                  Host/Reactivities:
                  Host: Mouse
                  Isotype/Clone:
                  Isotype: IgG1 / Clone QD14-504.5
                  Applications:
                  These antibodies have been qualified for use in ELISA to detect quinidine.
                  Testing Data TD
                  Quinidine antibody
                  Other Names:
                  Monoclonal Quinidine; Anti-Quinidine
                  Host/Reactivities:
                  Host: Mouse / Reactivity: To be determined by end-user
                  Isotype/Clone:
                  Isotype: IgG1 / Clone M310119
                  Applications:
                  ELISA (EIA)
                   
                  Quinidine Monoclonal Antibodies
                  Other Names:
                  Quinidine-Library Pack
                  Host/Reactivities:
                  Host: Mouse
                  Applications:
                  ELISA (EIA)
                  Testing Data
                  Mouse Anti Human Quinolinate Phosphoribosyltransferase
                  Gene Names:
                  QPRT
                  Other Names:
                  QPRT Antibody; Quinolinate phosphoribosyltransferase; QPRTase; QAPRTase; Mouse Anti Human
                  Host/Reactivities:
                  Host: Mouse / Reactivity: Human
                  Purification:
                  Antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
                  Isotype/Clone:
                  Isotype: IgG2a heavy chain and k light chain. / Clone PAT24C4AT
                  Applications:
                  ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
                   
                  Displaying 1 to 31 (of 31 products)
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