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Monoclonal Antibody

What is Monoclonal Antibody Purification?

Monoclonal antibody purification involves collecting the media from the cultured hybridomas, centrifuging and filtering the media, and then using chromatographic or physiochemical fractionation methods to isolate the antibodies. Physiochemical fractionation involves using differential precipitation, size-exclusion or solid-phase binding of proteins based on size, charge or other chemical characteristics. These techniques include Size Exclusion Chromotography (SEC), ammonium sulfate precipitation and Ion Exchange Chromotography. Antibodies can also be purified based on class-specific affinity which involves using immobilized agents, such as proteins or lectins, that have an affinity for the isotype of the antibody of interest. Antigen-affinity chromatography can also be used to purify antibodies, but this method is not commonly used for monoclonal antibodies since the target antibody is generally the only immunoglobulin in the sample.

Displaying 1 to 50 (of 4130 products) order by Products Name.
S tag
Host/Reactivities:
Host: Mouse
Purification:
ProG affinity purified
Isotype/Clone:
Isotype: IgG1 / Clone G10-C8
Applications:
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC)
 
S- Adenosylhomocysteine Antibody (Clone # 301-10)
Gene Names:
GARC_3658
Other Names:
S- Adenosylhomocysteine; S-adenosylhomocysteine
Host/Reactivities:
Host: Mouse / Reactivity: General
Purification:
Affinity chromatography purified
Isotype/Clone:
Isotype: IgG3 / Clone 301-10
Applications:
cELISA (EIA), Flow Ctometry (FC/FACS), Immunohistochemistry (IHC)
Testing Data
S- Adenosylmethionine Antibody (Clone # 118-18)
Other Names:
S- Adenosylmethionine; S-adenosylmethionine
Host/Reactivities:
Host: Mouse / Reactivity: General
Purification:
Affinity chromatography purified
Isotype/Clone:
Isotype: IgG2b / Clone 118-18
Applications:
cELISA (EIA), Flow Ctometry (FC/FACS), Immunohistochemistry (IHC)
Testing Data
S- Adenosylmethionine Antibody (Clone # 84-19)
Other Names:
S- Adenosylmethionine; S-adenosylmethionine
Host/Reactivities:
Host: Mouse / Reactivity: General
Purification:
Affinity chromatography purified
Isotype/Clone:
Isotype: IgG2b / Clone 84-19
Applications:
cELISA (EIA), Flow Ctometry (FC/FACS), Immunohistochemistry (IHC)
Testing Data
S- Adenosylmethionine Antibody (Clone # 118-6)
Other Names:
S- Adenosylmethionine; S-adenosylmethionine
Host/Reactivities:
Host: Mouse / Reactivity: General
Purification:
Affinity chromatography purified
Isotype/Clone:
Isotype: IgG2b / Clone 118-6
Applications:
cELISA (EIA), Flow Ctometry (FC/FACS), Immunohistochemistry (IHC)
Testing Data
S- Adenosylmethionine Antibody (Clone # 84-3)
Other Names:
S- Adenosylmethionine; S-adenosylmethionine
Host/Reactivities:
Host: Mouse / Reactivity: General
Purification:
Affinity chromatography purified
Isotype/Clone:
Isotype: IgG2b / Clone 84-3
Applications:
cELISA (EIA), Flow Ctometry (FC/FACS), Immunohistochemistry (IHC)
Testing Data
Anti-S-100
Gene Names:
S-100
Host/Reactivities:
Host: Rabbit / Reactivity: Human
Applications:
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Immunocytochemistry (ICC)
Anti - S-100
Gene Names:
S-100; S100A1; S100; S100A; S100-alpha
Other Names:
Protein S100-A1; S100 calcium binding protein A1
Host/Reactivities:
Host: Rabbit / Reactivity: Human
Applications:
Immunohistochemistry (IHC) - Formalin/Paraffin
  • Testing Data
  • Testing Data #2
  • Testing Data #3
    S-100
    Gene Names:
    S-100
    Other Names:
    S-100 calcium-binding protein A1; S100 calcium-binding proteinUniRule annotationAutomatic assertion according to rulesiRuleBase:RU361184
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a / Clone 4C4.9
     
    MAb to S100
    Gene Names:
    pgl; devB
    Other Names:
    Monoclonal Antibody to Human S100; S-100 calcium-binding protein A5 - human
    Host/Reactivities:
    Host: Host: MouseSource: Ascites / Reactivity: Human
    Purification:
    >70% pure (SDS-PAGE). Size exclusion chromatography.
    Isotype/Clone:
    Isotype: IgM, kappa / Clone 131-12211 (KJ22-1-1)
     
    S-100 Monoclonal Antibody
    Other Names:
    Anti-S-100; S100
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG1
    Applications:
    ELISA, Western Blot
     
    S-100 Monoclonal Antibody
    Other Names:
    Anti-S-100; S100
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG1
    Applications:
    ELISA, Western Blot
     
    S-100 Monoclonal Antibody
    Other Names:
    Anti-S-100; S100
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a
    Applications:
    ELISA, Western Blot
     
    S-100 Monoclonal Antibody
    Other Names:
    Anti-S-100; S100
    Host/Reactivities:
    Host: Mouse
    Isotype/Clone:
    Isotype: IgG2a
    Applications:
    ELISA, Western Blot
     
    MAb to S-100 (alpha-beta)
    Gene Names:
    pgl; devB
    Other Names:
    Monoclonal Antibody to Human S-100 (alpha-beta) protein; S-100 calcium-binding protein A5 - human
    Host/Reactivities:
    Host: Host: Mouse. Hybridization of Sp2/0 myelomacells with spleen cells from Balb/c mice.Source: ...
    Purification:
    >90% pure (SDS-PAGE). Protein A chromatography
    Isotype/Clone:
    Isotype: IgG2a / Clone 3B10
    Applications:
    EIA/ELISA, Western Blot
     
    MAb to S-100 (beta-beta)
    Gene Names:
    pgl; devB
    Other Names:
    Monoclonal Antibody to Human S-100 (beta-beta) protein; S-100 calcium-binding protein A5 - human
    Host/Reactivities:
    Host: Host: Mouse. Hybridization of Sp2/0 myelomacells with spleen cells from BALB/c mice.Source: ...
    Purification:
    >90% pure (SDS-PAGE). Protein A chromatography
    Isotype/Clone:
    Isotype: IgG1 / Clone 8B10
    Applications:
    EIA/ELISA, Immunoassay Antibody Pairs, Western Blot
     
    MAb to S-100 (beta-beta)
    Gene Names:
    pgl; devB
    Other Names:
    Monoclonal Antibody to Human S-100 (beta-beta); S-100 calcium-binding protein A5 - human
    Host/Reactivities:
    Host: Host: Mouse. Hybridization of Sp2/0 myelomacells with spleen cells of Balb/c mice.Source: As...
    Purification:
    >90% pure (SDS-PAGE). Protein A chromatography
    Isotype/Clone:
    Isotype: IgG2a / Clone 4B3
    Applications:
    Western Blot
     
    MAb to S-100 (beta-beta)
    Gene Names:
    pgl; devB
    Other Names:
    Monoclonal Antibody to Human S-100 (beta-beta) protein; S-100 calcium-binding protein A5 - human
    Host/Reactivities:
    Host: Host: Mouse. Hybridization of Sp2/0 myelomacells with spleen cells from BALB/c mice.Source: ...
    Purification:
    >90% pure (SDS-PAGE). Protein A chromatography
    Isotype/Clone:
    Isotype: IgG1 / Clone 6G1
    Applications:
    EIA/ELISA, Immunoassay Antibody Pairs, Western Blot
     
    S-100 Monoclonal
    Host/Reactivities:
    Host: Mouse / Reactivity: Human, Dog, Cat, Mouse, Rat, Cattle
    Isotype/Clone:
    Isotype: IgG2a / Clone 4C4.9
    • Dilution Information
    • Immunohistochemistry (IHC)
      Monoclonal mouse anti-human S-100 protein
      Purification:
      Chromatography on protein A Sepharose
      Isotype/Clone:
      Isotype: IgG1 for MAbs 8B10 and 6G1IgG2a for MAbs 3B10 and 4B3 / Clone 8B10, 6G1, 3B10, 4B3
       
      Anti-S-100(beta-subunit) antibody (monoclonal)
      Gene Names:
      S100B; NEF; S100; S100-B; S100beta
      Other Names:
      Protein S100-B; S100 calcium binding protein B; S100 calcium binding protein; beta(neural); S-100 protein beta chain; S-100 protein subunit beta
      Host/Reactivities:
      Host: Mouse / Reactivity: Human, Rabbit, Rat
      Purification:
      Ascites
      Isotype/Clone:
      Isotype: IgG1 / Clone SA-12
      Applications:
      Immunohistochemistry (IHC) Paraffin
      Immunohistochemistry (IHC)
      S-adenosylmethionine synthetase Mouse mAb for Cherry
      Gene Names:
      Desac_0208
      Other Names:
      SAMs Mouse mAb; S-adenosylmethionine synthetase
      Host/Reactivities:
      Host: Mouse / Reactivity: Cherry
      Isotype/Clone:
      Isotype: IgG
      Applications:
      Western Blot (WB)
      Western Blot (WB)
      S-Arrestin (SAG, S-AG, 48kD Protein, Retinal S-antigen, Rod Photoreceptor Arrestin)
      Gene Names:
      SAG; RP47; S-AG
      Other Names:
      Anti -S-Arrestin (SAG; 48kD Protein; Retinal S-antigen; Rod Photoreceptor Arrestin); S-arrestin; S-antigen; retina and pineal gland (arrestin)
      Host/Reactivities:
      Host: Mouse / Reactivity: Bovine, Human, Porcine, Rat
      Purification:
      Affinity PurifiedPurified by Protein G affinity chromatography.
      Isotype/Clone:
      Isotype: IgG1,k / Clone 13B857
      Applications:
      Western Blot (WB), Immunofluorescence (IF)
       
      S-Arrestin (S Arrestin, S-arrestin, 48kD Protein, DKFZp686I1383, Rod Photoreceptor Arrestin, S Antigen, S-antigen Retina and Pineal Gland, S AG, SAG, S-AG)
      Gene Names:
      SAG; RP47; S-AG; DKFZp686D1084; DKFZp686I1383
      Other Names:
      Anti -S-Arrestin (S Arrestin; S-arrestin; 48kD Protein; Rod Photoreceptor Arrestin; S Antigen; S-antigen Retina and Pineal Gland; S AG; S-AG); S-antigen; retina and pineal gland (arrestin)
      Host/Reactivities:
      Host: Mouse / Reactivity: Bovine, Human, Porcine
      Purification:
      PurifiedPurified
      Isotype/Clone:
      Isotype: IgG1 / Clone 9G8
      Applications:
      Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
       
      MOUSE ANTI S-TAG
      Gene Names:
      S-TAG
      Host/Reactivities:
      Host: Mouse
      Isotype/Clone:
      Isotype: IgG1 / Clone SBSTAGa
      Applications:
      ELISA (EIA), Flow cytometry (FC/FACS), Protein Purification (PP)
       
      MOUSE ANTI S-TAG
      Gene Names:
      S-TAG
      Host/Reactivities:
      Host: Mouse
      Isotype/Clone:
      Isotype: IgG2a / Clone SBSTAGb
      Applications:
      ELISA (EIA), Flow cytometry (FC/FACS), Protein Purification (PP)
       
      MOUSE ANTI S-TAG:FITC
      Gene Names:
      S-TAG
      Host/Reactivities:
      Host: Mouse
      Isotype/Clone:
      Isotype: IgG2b / Clone SBSTAGc
      Applications:
      Flow cytometry (FC/FACS)
       
      S-Tag Monoclonal Antibody
      Gene Names:
      S-Tag
       
      Anti-S-Tag-UNLB Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG1k
      Applications:
      Flow Cytometry (FC/FACS)
       
      Anti-S-Tag-FITC Conjugate Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG1k
      Applications:
      Flow Cytometry (FC/FACS)
       
      Anti-S-Tag-HRP Conjugate Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG1k
      Applications:
      ELISA (EIA)
       
      Anti-S-Tag-R-PE Conjugate Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG1k
      Applications:
      Flow Cytometry (FC/FACS)
       
      Anti-S-Tag-UNLB Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG2a kappa
      Applications:
      Flow Cytometry (FC/FACS)
       
      Anti-S-Tag-FITC Conjugate Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG2a kappa
      Applications:
      Flow Cytometry (FC/FACS)
       
      Anti-S-Tag-HRP Conjugate Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG2a kappa
      Applications:
      ELISA (EIA)
       
      Anti-S-Tag-Biotin Conjugate Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG2a kappa
      Applications:
      Flow Cytometry (FC/FACS)
       
      Anti-S-Tag-R-PE Conjugate Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG2a kappa
      Applications:
      Flow Cytometry (FC/FACS)
       
      Anti-S-Tag-UNLB Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG2b kappa
      Applications:
      Flow Cytometry (FC/FACS)
       
      Anti-S-Tag-FITC Conjugate Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG2b kappa
      Applications:
      Flow Cytometry (FC/FACS)
       
      Anti-S-Tag-HRP Conjugate Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG2b kappa
      Applications:
      ELISA (EIA)
       
      Anti-S-Tag-R-PE Conjugate Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity Purified
      Isotype/Clone:
      Isotype: IgG2b kappa
      Applications:
      Flow Cytometry (FC/FACS)
       
      S-Tag Mouse Monoclonal Antibody
      Host/Reactivities:
      Host: Mouse / Reactivity: All
      Purification:
      Affinity-Chromatography
      Isotype/Clone:
      Isotype: IgG
      Applications:
      Western Blot (WB), Peptide ELISA (EIA)
      Testing Data
      S-Tag Mouse Monoclonal Antibody
      Gene Names:
      S-Tag
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity-chromatography
      Isotype/Clone:
      Isotype: IgG1
      Applications:
      Western Blot (WB)
      Testing Data
      S-Tag Monoclonal Antibody
      Gene Names:
      S-Tag
      Other Names:
      S.Tag peptide (plasmid)
      Host/Reactivities:
      Host: Mouse
      Purification:
      Antigen Affinity Purified
      Isotype/Clone:
      Isotype: IgG
      Applications:
      Western Blot (WB)
      Western Blot (WB)
      S-Tag Monoclonal Antibody (3B3), HRP Conjugated
      Gene Names:
      S-Tag
      Other Names:
      S.Tag peptide (plasmid)
      Host/Reactivities:
      Host: Mouse
      Purification:
      Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      Isotype/Clone:
      Isotype: IgG
      Applications:
      Western Blot (WB)
       
      Anti-S-tag Antibody
      Gene Names:
      S
      Host/Reactivities:
      Host: Mouse
      Applications:
      Western Blot (WB)
      Western Blot (WB)
      S-Tag Mouse Monoclonal Antibody
      Gene Names:
      S-Tag
      Applications:
      Western Blot (WB)
      Testing Data
      Anti-S-tag Mouse mAb
      Gene Names:
      S-tag
      Host/Reactivities:
      Host: Mouse / Reactivity: Human, Rat, Mouse
      Purification:
      Fractional precipitation
      Applications:
      Western Blot (WB)
       
      MAb to S. Enterica paratyphi
      Other Names:
      Monoclonal Antibody to Salmonella enterica paratyphi; Salmonella Enterica paratyphi
      Host/Reactivities:
      Host: Mouse
      Purification:
      >90% pure (SDS-PAGE)Protein G Chromatography
      Isotype/Clone:
      Isotype: IgG3
      Applications:
      ELISA (EIA), Lateral Flow, Western Blot (WB)
       
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