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Cxcl12 elisa kit :: Mouse Stromal cell derived factor 1/SDF-1 ELISA Kit

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Catalog # MBS175856
Unit / Price DISCONTINUED
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Stromal cell derived factor 1/SDF-1 (Cxcl12), ELISA Kit

Popular Item
Also Known As

Mouse SDF-1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sequence Length
119
Chromosome Location
Chromosome: 6; NC_000072.6 (117168535..117181368). Location: 6 F1; 6 54.81 cM
3D Structure
ModBase 3D Structure for H7BX38
Species Reactivity
Specificity
Natural and recombinant mouse SDF-1
Samples
For quantitative detection of mouse SDF-1 in cell culture supernates, serum and plasma(heparin).
Detection Range
62.5pg/ml-4000pg/ml
Samples
For quantitative detection of mouse SDF-1 in cell culture supernates, serum and plasma(heparin).
Detection Range
62.5pg/ml-4000pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Cxcl12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forCxcl12purchase
MBS175856 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Stromal cell derived factor 1/SDF-1 (Cxcl12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cxcl12. The ELISA analytical biochemical technique of the MBS175856 kit is based on Cxcl12 antibody-Cxcl12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cxcl12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Cxcl12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Cxcl12 elisa kit
Principle of the Assay: mouse SDF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SDF-1 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SDF-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SDF-1 amount of sample captured in plate.

Background: SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). This gene is located on chromosome 10q11.1.1 SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alternate splicing of the same gene.2 Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes.3,4,5,6 CXCL12 was shown to be expressed in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow). CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes.7 CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide.8 The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8Kda.

Typical Testing Data/Standard Curve (for reference only) of Cxcl12 elisa kit
Cxcl12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175856. Click to request current manual
NCBI/Uniprot data below describe general gene information for Cxcl12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
13,634 Da[Similar Products]
NCBI Official Full Name
stromal cell-derived factor 1 isoform gamma
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 12
NCBI Official Symbol
Cxcl12  [Similar Products]
NCBI Official Synonym Symbols
Pbsf; Sdf1; Tlsf; Tpar1; Scyb12
  [Similar Products]
NCBI Protein Information
stromal cell-derived factor 1; pre-B-cell growth-stimulating factor; thymic lymphoma cell-stimulating factor; 12-O-tetradecanoylphorbol 13-acetate repressed protein 1
UniProt Protein Name
Stromal cell-derived factor 1
UniProt Gene Name
Cxcl12  [Similar Products]
UniProt Entry Name
H7BX38_MOUSE
Product References and Citations for Cxcl12 elisa kit
1. Shirozu, M.; Nakano, T.; Inazawa, J.; Tashiro, K.; Tada, H.; Shinohara, T.; Honjo, T. : Structure and chromosomal localization of the human stromal cell-derived factor 1 (SDF1) gene. Genomics 28: 495-500, 1995.
2. De La Luz Sierra et al. Differential processing of stromal-derived factor-1alpha and beta explains functional diversity. Blood 103:2452-2459, 2004.
3. Bleul et al. A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1). J. Exp. Med. 184: 1101-1109, 1996.
4. Ara et al. Impaired colonization of the gonads by primordial germ cells in mice lacking a chemokine, stromal cell-derived factor-1 (SDF-1). Proc. Nat. Acad. Sci. 100: 5319-5323, 2003.
5. Askari et al. Effect of stromal-cell-derived factor 1 on stem-cell homing and tissue regeneration in ischaemic cardiomyopathy. Lancet 362: 697-703, 2003.
6. Ma et al. Impaired B-lymphopoiesis, myelopoiesis, and derailed cerebellar neuron migration in CXCR4- and SDF-1-deficient mice. Proc. Nat. Acad. Sci. 95: 9448-9453, 1998.
7. Bleul, C. C.; Fuhlbrigge, R. C.; Casasnovas, J. M.; Aiuti, A.; Springer, T. A. : A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1). J. Exp. Med. 184: 1101-1109, 1996.
8. McQuibban, G. A.; Butler, G. S.; Gong, J. H.; Bendall, L.; Power, C.; Clark-Lewis, I.; Overall, C. M. : Matrix metalloproteinase activity inactivates the CXC chemokine stromal cell-derived factor-1. J. Biol. Chem. 276: 43503-43508, 2001.

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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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