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MS4A1 elisa kit :: Human Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) ELISA Kit

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Catalog # MBS455171
Unit / Price
  48-Strip-Wells  /  $385 +1 FREE 8GB USB
  96-Strip-Wells  /  $515 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,325 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,515 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1), ELISA Kit

Popular Item
Also Known As

Human Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
297
OMIM
X12530 mRNA
3D Structure
ModBase 3D Structure for P11836
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of MS4A1. No significant cross-reactivity or interference between MS4A1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
1.25-80ng/mL
Sensitivity
0.45ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MS4A1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MS4A1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MS4A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMS4A1purchase
MBS455171 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MS4A1. The ELISA analytical biochemical technique of the MBS455171 kit is based on MS4A1 antibody-MS4A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MS4A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MS4A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MS4A1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MS4A1 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MS4A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MS4A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MS4A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MS4A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of MS4A1 elisa kit
MS4A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS455171. Click to request current manual
NCBI/Uniprot data below describe general gene information for MS4A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,581 Da
NCBI Official Full Name
B-lymphocyte antigen CD20
NCBI Official Synonym Full Names
membrane spanning 4-domains A1
NCBI Official Symbol
MS4A1  [Similar Products]
NCBI Official Synonym Symbols
B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
  [Similar Products]
NCBI Protein Information
B-lymphocyte antigen CD20
UniProt Protein Name
B-lymphocyte antigen CD20
UniProt Synonym Protein Names
B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD_antigen: CD20
Protein Family
UniProt Gene Name
MS4A1  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for MS4A1
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
UniProt Comments for MS4A1
This protein may be involved in the regulation of B-cell activation and proliferation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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