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MT2 recombinant protein :: Metallothionein 2 (MT2) Recombinant Protein

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Catalog # MBS2012291 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $115 +1 FREE 8GB USB
  0.05 mg  /  $200 +1 FREE 8GB USB
  0.1 mg  /  $290 +1 FREE 8GB USB
  0.2 mg  /  $360 +1 FREE 8GB USB
  0.5 mg  /  $690 +1 FREE 8GB USB
  1 mg  /  $1,015 +1 FREE 8GB USB
SDS-Page
Product Name

Metallothionein 2 (MT2), Recombinant Protein

Popular Item
Also Known As

Recombinant Metallothionein 2 (MT2)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2012291 Technical Datasheet
Chromosome Location
Chromosome: 16; NC_000016.9 (56642478..56643409). Location: 16q13
OMIM
156360
3D Structure
ModBase 3D Structure for P02795
Host
E Coli
Purity/Purification
>90%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Concentration
Original Concentration: 200 Ug/mL (lot specific)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Asp2~Ala61
Tags
No-Tag
Traits
Freeze-dried powder
Buffer formulation
100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Predicted isoelectric point
8.3
Predicted Molecular Mass
5.9kDa
Accurate Molecular Mass
12kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
USAGE
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of MT2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MT2 recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for MT2 recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB.

(May be suitable for use in other assays to be determined by the end user.)

NCBI/Uniprot data below describe general gene information for MT2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
metallothionein-2
NCBI Official Synonym Full Names
metallothionein 2A
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
metallothionein-2; MT-2; MT-II; metallothionein-2A; metallothionein-II
UniProt Protein Name
Metallothionein-2
UniProt Synonym Protein Names
Metallothionein-2A; Metallothionein-II
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CES1; MT2; MT-2; MT-II  [Similar Products]
UniProt Entry Name
MT2_HUMAN
UniProt Comments for MT2
MT2A: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. Belongs to the metallothionein superfamily. Type 1 family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; zinc ion binding; drug binding

Biological Process: cellular copper ion homeostasis; cytokine and chemokine mediated signaling pathway; negative regulation of growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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