• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

MTHFR elisa kit :: Chicken MTHFR ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2507478
Unit / Price
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name


Popular Item
Also Known As

Chicken MTHFR (Methylenetetrahydrofolate Reductase) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
3D Structure
ModBase 3D Structure for P42898
Species Reactivity
This kit recognizes natural and recombinant Chicken MTHFR. No significant cross-reactivity or interference between Chicken MTHFR and analogues was observed.
Serum, Plasma, Biological Fluids
Assay Type
Detection Range
Min: 2.344pg/mL ; Max: 250pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MTHFR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMTHFRpurchase
MBS2507478 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MTHFR, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MTHFR. The ELISA analytical biochemical technique of the MBS2507478 kit is based on MTHFR antibody-MTHFR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MTHFR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MTHFR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MTHFR elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken MTHFR concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MTHFR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MTHFR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MTHFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MTHFR. You can calculate the concentration of MTHFR in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of MTHFR elisa kit
MTHFR elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2507478. Click to request current manual
NCBI/Uniprot data below describe general gene information for MTHFR. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
78,965 Da
NCBI Official Full Name
methylenetetrahydrofolate reductase
NCBI Official Synonym Full Names
methylenetetrahydrofolate reductase (NAD(P)H)
NCBI Official Symbol
MTHFR  [Similar Products]
NCBI Protein Information
methylenetetrahydrofolate reductase; 5,10-methylenetetrahydrofolate reductase (NADPH)
UniProt Protein Name
Methylenetetrahydrofolate reductase
UniProt Gene Name
MTHFR  [Similar Products]
UniProt Entry Name
NCBI Summary for MTHFR
The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.[provided by RefSeq, Oct 2009]
UniProt Comments for MTHFR
MTHFR: Catalyzes the conversion of 5,10- methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co- substrate for homocysteine remethylation to methionine. Defects in MTHFR are the cause of methylenetetrahydrofolate reductase deficiency (MTHFRD). MTHFRD is autosomal recessive disorder with a wide range of features including homocysteinuria, homocysteinemia, developmental delay, severe mental retardation, perinatal death, psychiatric disturbances, and later-onset neurodegenerative disorders. Defects in MTHFR may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in MTHFR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Belongs to the methylenetetrahydrofolate reductase family.

Protein type: Energy Metabolism - methane; EC; Oxidoreductase; Cofactor and Vitamin Metabolism - one carbon pool by folate

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: neuron projection; cytosol

Molecular Function: FAD binding; NADP binding; methylenetetrahydrofolate reductase (NADPH) activity

Biological Process: response to drug; amino acid metabolic process; vitamin metabolic process; methionine biosynthetic process; blood circulation; response to folic acid; response to vitamin B2; response to hypoxia; S-adenosylmethionine metabolic process; homocysteine metabolic process; one-carbon compound metabolic process; folic acid metabolic process; water-soluble vitamin metabolic process

Disease: Schizophrenia; Thrombophilia Due To Thrombin Defect; Neural Tube Defects, Folate-sensitive; Homocystinuria Due To Deficiency Of N(5,10)-methylenetetrahydrofolate Reductase Activity
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

Contact Us

Please fill out the form below and our representative will get back to you shortly.