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MTR elisa kit :: Human Methionine synthase ELISA Kit

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Catalog # MBS763612
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Methionine synthase (MTR), ELISA Kit

Popular Item
Also Known As

Human Methionine synthase ELISA Kit

Product Synonym Names
MTR/Methionine synthase/5-methyltetrahydrofolate--homocysteine methyltransferase/Vitamin-B12 dependent methionine synthase/MS
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS763612 COA
MBS763612 QC Report
Sequence Length
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of MTR. No significant cross-reactivity or interference between MTR and analogues was observed.
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MTR were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MTR were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MTR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMTRpurchase
MBS763612 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Methionine synthase (MTR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MTR. The ELISA analytical biochemical technique of the MBS763612 kit is based on MTR antibody-MTR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MTR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MTR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MTR elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MTR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MTR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MTR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MTR can be calculated.

Typical Testing Data/Standard Curve (for reference only) of MTR elisa kit
MTR elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS763612. Click to request current manual
NCBI/Uniprot data below describe general gene information for MTR. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
134,793 Da
NCBI Official Full Name
Methionine synthase
NCBI Official Synonym Full Names
5-methyltetrahydrofolate-homocysteine methyltransferase
NCBI Official Symbol
NCBI Official Synonym Symbols
MS; HMAG; cblG
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NCBI Protein Information
methionine synthase
UniProt Protein Name
Methionine synthase
UniProt Synonym Protein Names
5-methyltetrahydrofolate--homocysteine methyltransferase; Vitamin-B12 dependent methionine synthase; MS
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
NCBI Summary for MTR
This gene encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Comments for MTR
MTR: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. Defects in MTR are the cause of methylcobalamin deficiency type G (cblG); also known as homocystinuria-megaloblastic anemia complementation type G. It is an autosomal recessive inherited disease that causes mental retardation, macrocytic anemia, and homocystinuria. Mild deficiency in MS activity could be associated with mild hyperhomocysteinemia, a risk factor for cardiovascular disease and possibly neural tube defects. MS mutations could also be involved in tumorigenesis. Defects in MTR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTR may affect the risk of spina bifida via the maternal rather than the embryonic genotype. Belongs to the vitamin-B12 dependent methionine synthase family.

Protein type: Amino Acid Metabolism - cysteine and methionine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC; Methyltransferase

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: cytoplasm; cytosol

Molecular Function: cobalamin binding; homocysteine S-methyltransferase activity; methionine synthase activity; protein binding; zinc ion binding

Biological Process: axon regeneration; cobalamin metabolic process; methionine biosynthetic process; methylation; nervous system development; pteridine and derivative metabolic process; response to axon injury; sulfur amino acid metabolic process; vitamin metabolic process; water-soluble vitamin metabolic process; xenobiotic metabolic process

Disease: Homocystinuria-megaloblastic Anemia, Cblg Complementation Type; Neural Tube Defects, Folate-sensitive
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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