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MTR elisa kit :: Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase ELISA Kit

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Catalog # MBS9325332
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MTR elisa kit
Product Name

5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), ELISA Kit

Popular Item
Full Product Name

Mouse Methionine synthase, MTR ELISA Kit

Product Synonym Names
Mouse Methionine synthase (MTR) ELISA kit; FLJ33168; FLJ43216; FLJ45386; MS; cblG; 5-methyltetrahydrofolate-homocysteine methyltransferase 1; cobalamin-dependent methionine synthase; methionine synthase; vitamin-B12 dependent methionine synthas; 5-methyltetrahydrofolate-homocysteine methyltransferase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for A6H5Y3
Species Reactivity
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Samples
Body fluids, tissue homogenates, secretions or feces samples
Assay Type
Quantitative Sandwich
Detection Range
0.25 ng/ml - 8 ng/ml
Sensitivity
0.1 ng/ml.
Intra-assay Precision
Intra-assay CV (%) is less than 15%
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MTR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MTRpurchase
MBS9325332 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MTR. The ELISA analytical biochemical technique of the MBS9325332 kit is based on MTR antibody-MTR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MTR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MTR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MTR elisa kit
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of MTR (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS9325332. Click to request current manual
NCBI/Uniprot data below describe general gene information for MTR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
139,069 Da
NCBI Official Full Name
methionine synthase
NCBI Official Synonym Full Names
5-methyltetrahydrofolate-homocysteine methyltransferase
NCBI Official Symbol
NCBI Official Synonym Symbols
MS; AI894170; D830038K18Rik
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NCBI Protein Information
methionine synthase; vitamin-B12 dependent methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase
UniProt Protein Name
Methionine synthase
UniProt Synonym Protein Names
5-methyltetrahydrofolate--homocysteine methyltransferase; Vitamin-B12 dependent methionine synthase; MS
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
METH_MOUSE
UniProt Comments for MTR
MTR: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate. Defects in MTR are the cause of methylcobalamin deficiency type G (cblG); also known as homocystinuria-megaloblastic anemia complementation type G. It is an autosomal recessive inherited disease that causes mental retardation, macrocytic anemia, and homocystinuria. Mild deficiency in MS activity could be associated with mild hyperhomocysteinemia, a risk factor for cardiovascular disease and possibly neural tube defects. MS mutations could also be involved in tumorigenesis. Defects in MTR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTR may affect the risk of spina bifida via the maternal rather than the embryonic genotype. Belongs to the vitamin-B12 dependent methionine synthase family.

Protein type: Methyltransferase; Amino Acid Metabolism - cysteine and methionine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.1.13

Cellular Component: cytoplasm; intracellular; cytosol

Molecular Function: transferase activity; amino acid binding; methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; metal ion binding; methionine synthase activity; cobalamin binding; folic acid binding

Biological Process: tetrahydrofolate metabolic process; methylation; cellular metabolic process; protein amino acid methylation; methionine biosynthetic process; pteridine and derivative metabolic process; methionine metabolic process; amino acid biosynthetic process; homocysteine metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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