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MUC5AC elisa kit :: Human Mucin 5 Subtype AC (MUC5AC) ELISA Kit

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Catalog # MBS454469
Unit / Price
  48-Strip-Wells  /  $375 +1 FREE 8GB USB
  96-Strip-Wells  /  $495 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,225 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,320 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Mucin 5 Subtype AC (MUC5AC), ELISA Kit

Popular Item
Full Product Name

Human Mucin 5 Subtype AC (MUC5AC) ELISA Kit

Product Synonym Names
TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
FO680660 Genomic DNA
3D Structure
ModBase 3D Structure for P98088
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of MUC5AC. No significant cross-reactivity or interference between MUC5AC and analogues was observed.
Samples
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type
Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
28pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MUC5AC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MUC5AC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MUC5AC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMUC5ACpurchase
MBS454469 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mucin 5 Subtype AC (MUC5AC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MUC5AC. The ELISA analytical biochemical technique of the MBS454469 kit is based on MUC5AC antibody-MUC5AC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MUC5AC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MUC5AC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MUC5AC elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MUC5AC in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MUC5AC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MUC5AC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MUC5AC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MUC5AC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of MUC5AC elisa kit
MUC5AC elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS454469. Click to request current manual
NCBI/Uniprot data below describe general gene information for MUC5AC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
585,570 Da
NCBI Official Full Name
mucin-5AC
NCBI Official Synonym Full Names
mucin 5AC, oligomeric mucus/gel-forming
NCBI Official Symbol
MUC5AC  [Similar Products]
NCBI Official Synonym Symbols
TBM; leB; MUC5; mucin
  [Similar Products]
NCBI Protein Information
mucin-5AC
UniProt Protein Name
Mucin-5AC
UniProt Synonym Protein Names
Gastric mucin
Protein Family
UniProt Gene Name
MUC5AC  [Similar Products]
UniProt Synonym Gene Names
UniProt Comments for MUC5AC
Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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