Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List MUC5AC elisa kit :: Human Mucin 5 Subtype AC (MUC5AC) ELISA Kit Catalog # MBS454469 Unit / Price 48-Strip-Wells / $375 +1 FREE 8GB USB 96-Strip-Wells / $495 +1 FREE 8GB USB 5x96-Strip-Wells / $2,225 +2 FREE 8GB USB 10x96-Strip-Wells / $4,320 +5 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Mucin 5 Subtype AC (MUC5AC), ELISA Kit Popular Item Full Product Name Human Mucin 5 Subtype AC (MUC5AC) ELISA Kit Product Synonym Names TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein Product Gene Name MUC5AC elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Request for Current Manual Insert Request Current Manual OMIM FO680660 Genomic DNA 3D Structure ModBase 3D Structure for P98088 Species Reactivity Human Specificity This assay has high sensitivity and excellent specificity for detection of MUC5AC. No significant cross-reactivity or interference between MUC5AC and analogues was observed. Samples Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type Sandwich Detection Range 78.125-5000pg/mL Sensitivity 28pg/mL Intra-assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MUC5AC were tested 20 times on one plate, respectively. Intra-Assay: CV<10% Inter-assay Precision Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MUC5AC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12% Preparation and Storage For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C. ISO Certification Manufactured in an ISO 9001:2008 Certified Laboratory. Product Note Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual. Other Notes Small volumes of MUC5AC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms forMUC5ACpurchase MBS454469 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mucin 5 Subtype AC (MUC5AC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MUC5AC. The ELISA analytical biochemical technique of the MBS454469 kit is based on MUC5AC antibody-MUC5AC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MUC5AC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MUC5AC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for MUC5AC elisa kit Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MUC5AC in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MUC5AC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MUC5AC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MUC5AC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MUC5AC in the samples is then determined by comparing the O.D. of the samples to the standard curve. Typical Testing Data/Standard Curve (for reference only) of MUC5AC elisa kit TOP Sample Manual Insert of MBS454469. Click to request current manual NCBI/Uniprot data below describe general gene information for MUC5AC. It may not necessarily be applicable to this product. NCBI GI # 748983076 NCBI GeneID 4586 NCBI Accession # NP_001291288.1 [Other Products] NCBI GenBank Nucleotide # NM_001304359.1 [Other Products] UniProt Primary Accession # P98088 [Other Products] UniProt Secondary Accession # O60460; O76065; Q13792; Q14425; Q658Q1; Q7M4S5; Q8N4M9; Q8WWQ3; Q8WWQ4; Q8WWQ5; A0A096LPK4[Other Products] UniProt Related Accession # P98088[Other Products] Molecular Weight 585,570 Da TOP NCBI Official Full Name mucin-5AC NCBI Official Synonym Full Names mucin 5AC, oligomeric mucus/gel-forming NCBI Official Symbol MUC5AC [Similar Products] NCBI Official Synonym Symbols TBM; leB; MUC5; mucin [Similar Products] NCBI Protein Information mucin-5AC UniProt Protein Name Mucin-5AC UniProt Synonym Protein Names Gastric mucin Protein Family Mucin UniProt Gene Name MUC5AC [Similar Products] UniProt Synonym Gene Names TBM [Similar Products] TOP TOP UniProt Comments for MUC5AC Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. Research Articles on MUC5AC 1. this study shows that SYK increased MUC5AC expression via ERK2 and p38 MAPK signaling pathways in airway epithelial cells TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Pathways associated with MUC5AC elisa kit Products by Pathway Pathway Diagram C-type Lectin Receptors (CLRs) Pathway antibodies C-type Lectin Receptors (CLRs) Pathway Diagram Dectin-2 Family Pathway antibodies Dectin-2 Family Pathway Diagram Defective C1GALT1C1 Causes Tn Polyagglutination Syndrome (TNPS) Pathway antibodies Defective C1GALT1C1 Causes Tn Polyagglutination Syndrome (TNPS) Pathway Diagram Defective GALNT12 Causes Colorectal Cancer 1 (CRCS1) Pathway antibodies Defective GALNT12 Causes Colorectal Cancer 1 (CRCS1) Pathway Diagram Defective GALNT3 Causes Familial Hyperphosphatemic Tumoral Calcinosis (HFTC) Pathway antibodies Defective GALNT3 Causes Familial Hyperphosphatemic Tumoral Calcinosis (HFTC) Pathway Diagram Disease Pathway antibodies Disease Pathway Diagram Diseases Associated With O-glycosylation Of Proteins Pathway antibodies Diseases Associated With O-glycosylation Of Proteins Pathway Diagram Diseases Of Glycosylation Pathway antibodies Diseases Of Glycosylation Pathway Diagram Immune System Pathway antibodies Immune System Pathway Diagram Innate Immune System Pathway antibodies Innate Immune System Pathway Diagram Diseases associated with MUC5AC elisa kit Disease Name Pubmed Publications Lung Diseases Antibodies >415 publications with MUC5AC and Lung Diseases Inflammation Antibodies >396 publications with MUC5AC and Inflammation Asthma Antibodies >276 publications with MUC5AC and Asthma Metaplasia Antibodies >262 publications with MUC5AC and Metaplasia Hypersensitivity Antibodies >177 publications with MUC5AC and Hypersensitivity Hyperplasia Antibodies >172 publications with MUC5AC and Hyperplasia Respiratory Hypersensitivity Antibodies >161 publications with MUC5AC and Respiratory Hypersensitivity Necrosis Antibodies >137 publications with MUC5AC and Necrosis Disease Models, Animal Antibodies >136 publications with MUC5AC and Disease Models, Animal Fibrosis Antibodies >122 publications with MUC5AC and Fibrosis Organs/Tissues associated with MUC5AC elisa kit Organ/Tissue Name Pubmed Publications Lung Antibodies >546 publications with MUC5AC and Lung Intestine Antibodies >380 publications with MUC5AC and Intestine Stomach Antibodies >368 publications with MUC5AC and Stomach Eye Antibodies >205 publications with MUC5AC and Eye Pancreas Antibodies >129 publications with MUC5AC and Pancreas Mammary Gland Antibodies >4 publications with MUC5AC and Mammary Gland Uncharacterized Tissue Antibodies >1 publications with MUC5AC and Uncharacterized Tissue TOP © 2006-18 MyBioSource.com. 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