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MYEF2 elisa kit :: Human myelin expression factor 2 ELISA Kit

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Catalog # MBS9318813
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
MYEF2 elisa kit
Product Name

myelin expression factor 2 (MYEF2), ELISA Kit

Also Known As

Human Myelin expression factor 2, MYEF2 ELISA Kit

Product Synonym Names
Human Myelin expression factor 2 (MYEF2) ELISA kit; FLJ11213; HsT18564; KIAA1341; MEF-2; MGC87325; MST156; MSTP156; myelin gene expression factor 2; myelin expression factor 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q9P2K5
Species Reactivity
Specificity
No significant cross-reactivity or interference between Human MYEF2 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
31.2pg/ml-1000pg/ml.
Sensitivity
5.0pg/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MYEF2 concentrations in Human serum, plasma and other body fluids. Using Purified Human MYEF2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MYEF2 and MYEF2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MYEF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MYEF2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMYEF2purchase
MBS9318813 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the myelin expression factor 2 (MYEF2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MYEF2. The ELISA analytical biochemical technique of the MBS9318813 kit is based on MYEF2 antibody-MYEF2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MYEF2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MYEF2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MYEF2 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MYEF2 concentrations in Human serum, plasma and other body fluids. Using Purified Human MYEF2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MYEF2 and MYEF2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MYEF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS9318813. Click to request current manual
NCBI/Uniprot data below describe general gene information for MYEF2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
64,122 Da
NCBI Official Full Name
myelin expression factor 2 isoform a
NCBI Official Synonym Full Names
myelin expression factor 2
NCBI Official Symbol
MYEF2  [Similar Products]
NCBI Official Synonym Symbols
MEF-2; MST156; myEF-2; MSTP156; HsT18564
  [Similar Products]
NCBI Protein Information
myelin expression factor 2; myelin gene expression factor 2
UniProt Protein Name
Myelin expression factor 2
UniProt Synonym Protein Names
MST156
Protein Family
UniProt Gene Name
MYEF2  [Similar Products]
UniProt Synonym Gene Names
KIAA1341; MEF-2; MyEF-2  [Similar Products]
UniProt Entry Name
MYEF2_HUMAN
UniProt Comments for MYEF2
MYEF2: Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: DNA binding; nucleotide binding

Biological Process: neuron differentiation; transcription, DNA-dependent; myotube differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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