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MYH1 elisa kit :: Mouse Myosin-1 (MYH1) ELISA Kit

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Catalog # MBS7215467
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Myosin-1 (MYH1), ELISA Kit

Popular Item
Full Product Name

Mouse Myosin-1 (MYH1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
160730
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of MYH1. No significant cross-reactivity or interference between MYH1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MYH1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This MYH1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MYH1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MYH1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forMYH1purchase
MBS7215467 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myosin-1 (MYH1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MYH1. The ELISA analytical biochemical technique of the MBS7215467 kit is based on MYH1 antibody-MYH1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MYH1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MYH1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
MYH1 elisa kit
Principle of the assay: MYH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MYH1 antibody and an MYH1-HRP conjugate. The assay sample and buffer are incubated together with MYH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYH1 concentration since MYH1 from samples and MYH1-HRP conjugate compete for the anti-MYH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MYH1 from the sample, fewer sites are left to bind MYH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYH1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MYH1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of MYH1 elisa kit
MYH1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7215467. Click to request current manual
NCBI/Uniprot data below describe general gene information for MYH1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
223,145 Da
NCBI Official Full Name
myosin-1
NCBI Official Synonym Full Names
myosin, heavy chain 1, skeletal muscle, adult
NCBI Official Symbol
NCBI Official Synonym Symbols
MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D
  [Similar Products]
NCBI Protein Information
myosin-1; myHC-IIx/d; myosin heavy chain 1; myosin heavy chain 2x; myosin heavy chain IIx/d; epididymis luminal protein 71; myosin heavy chain, skeletal muscle, adult 1; myosin, heavy polypeptide 1, skeletal muscle, adult
UniProt Protein Name
Myosin-1
UniProt Synonym Protein Names
Myosin heavy chain 1; Myosin heavy chain 2x; MyHC-2x; Myosin heavy chain IIx/d; MyHC-IIx/d; Myosin heavy chain, skeletal muscle, adult 1
UniProt Gene Name
UniProt Synonym Gene Names
MyHC-2x; MyHC-IIx/d  [Similar Products]
UniProt Entry Name
MYH1_HUMAN
NCBI Summary for MYH1
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]
UniProt Comments for MYH1
MYH1: Muscle contraction.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: muscle myosin complex; A band

Molecular Function: calmodulin binding; motor activity; actin binding; ATP binding

Biological Process: muscle contraction; metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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