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MYPT1 elisa kit

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Catalog # MBS9501127
Unit / Price
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  2x96 Wells  /  $535 +1 FREE 8GB USB
MYPT1 elisa kit
Product Name

MYPT1, ELISA Kit

Popular Item
Full Product Name

Phospho-MYPT1 (Thr853) Colorimetric Cell-Based ELISA Kit

Product Synonym Names
MBS; Myosin phosphatase target subunit 1; PPP1R12A
Product Gene Name
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
D87930 mRNA
3D Structure
ModBase 3D Structure for O14974
Species Reactivity
Form/Format
Two 96-Well Plates
Samples
Researchers plate their cell line of choice
Assay Type
Phospho Cell-Based ELISA
Sensitivity
>=5000 cells/well
Reactivity Note
Human: T853, Mouse: T852, Rat: T855
Detection Method
Colorimetric 450 nm
Preparation and Storage
Store at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MYPT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MYPT1purchase
MBS9501127 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MYPT1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MYPT1. The ELISA analytical biochemical technique of the MBS9501127 kit is based on MYPT1 antibody-MYPT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MYPT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MYPT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for MYPT1 elisa kit
Sample Manual Insert of MBS9501127. Click to request current manual
NCBI/Uniprot data below describe general gene information for MYPT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
115281
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 12A isoform a
NCBI Official Synonym Full Names
protein phosphatase 1 regulatory subunit 12A
NCBI Official Symbol
PPP1R12A  [Similar Products]
NCBI Official Synonym Symbols
MBS; M130; MYPT1
  [Similar Products]
NCBI Protein Information
protein phosphatase 1 regulatory subunit 12A
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 12A
UniProt Synonym Protein Names
Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit
UniProt Gene Name
PPP1R12A  [Similar Products]
UniProt Synonym Gene Names
Myosin phosphatase target subunit 1  [Similar Products]
NCBI Summary for MYPT1
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]
UniProt Comments for MYPT1
MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q21.2-q21.31

Cellular Component: A band; actin cytoskeleton; centrosome; contractile fiber; cytosol; focal adhesion; kinetochore; nucleoplasm; PTW/PP1 phosphatase complex; Z disc

Molecular Function: 14-3-3 protein binding; enzyme inhibitor activity; phosphatase regulator activity; protein binding; protein kinase binding; signal transducer activity

Biological Process: centrosome cycle; G2/M transition of mitotic cell cycle; mitotic cell cycle; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; regulation of cell adhesion; regulation of nucleocytoplasmic transport; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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