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NAG elisa kit :: Rat N-acetyl-beta-D-glucosaminidase, NAG ELISA Kit

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Catalog # MBS702746
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

N-acetyl-beta-D-glucosaminidase, NAG, ELISA Kit

Popular Item
Full Product Name

Rat N-acetyl-beta-D-glucosaminidase, NAG ELISA Kit

Product Synonym Names
Rat N-acetyl-beta-D-glucosaminidase; NAG ELISA Kit; ENC-1AS; N-acetyl-beta-glucosaminidase; hexosaminidase B; Beta-hexosaminidase subunit beta (HEXB) ; N-acetyl-beta-D-glucosaminidase; NAG
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS702746 Testing Data
3D Structure
ModBase 3D Structure for Q6AXR4
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat NAG. No significant cross-reactivity or interference between Rat NAG and analogues was observed.
Samples
Serum, plasma, cell culture supernates, tissue homogenates, urine
Assay Type
Sandwich
Detection Range
1.56 mIU/ml-100 mIU/ml
Sensitivity
0.39 mIU/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NAG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNAGpurchase
MBS702746 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the N-acetyl-beta-D-glucosaminidase, NAG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NAG. The ELISA analytical biochemical technique of the MBS702746 kit is based on NAG antibody-NAG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NAG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NAG elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NAG elisa kit
NAG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS702746. Click to request current manual
NCBI/Uniprot data below describe general gene information for NAG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
61,527 Da
NCBI Official Full Name
beta-hexosaminidase subunit beta
NCBI Official Synonym Full Names
hexosaminidase B
NCBI Official Symbol
NCBI Protein Information
beta-hexosaminidase subunit beta; hexosaminidase subunit B; beta-N-acetylhexosaminidase subunit beta; N-acetyl-beta-glucosaminidase subunit beta
UniProt Protein Name
Beta-hexosaminidase subunit beta
UniProt Synonym Protein Names
Beta-N-acetylhexosaminidase subunit beta; Hexosaminidase subunit B; N-acetyl-beta-glucosaminidase subunit beta
UniProt Gene Name
UniProt Synonym Gene Names
Hexosaminidase subunit B  [Similar Products]
UniProt Entry Name
HEXB_RAT
UniProt Comments for NAG
Function: Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues

Catalytic activity: Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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