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Nanog elisa kit :: Mouse Nanog homeobox ELISA Kit

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Catalog # MBS2602965
Unit / Price
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  48-Strip-Wells  /  $275 +1 FREE 8GB USB
  96-Strip-Wells  /  $435 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Nanog homeobox, ELISA Kit

Full Product Name

Mouse NANOG Homeobox protein, NANOG ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q80Z64
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant NANOG concentration
Assay Type
Sandwich
Detection Range
100 ng/ml-1.56 ng/ml
Sensitivity
0.5 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Nanog elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNanogpurchase
MBS2602965 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nanog homeobox, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Nanog. The ELISA analytical biochemical technique of the MBS2602965 kit is based on Nanog antibody-Nanog antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Nanog antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Nanog. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Nanog elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse NANOG monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve (for reference only) of Nanog elisa kit
Nanog elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2602965. Click to request current manual
NCBI/Uniprot data below describe general gene information for Nanog. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,240 Da[Similar Products]
NCBI Official Full Name
homeobox protein NANOG isoform 2
NCBI Official Synonym Full Names
Nanog homeobox
NCBI Official Symbol
Nanog  [Similar Products]
NCBI Official Synonym Symbols
ENK; ecat4; 2410002E02Rik
  [Similar Products]
NCBI Protein Information
homeobox protein NANOG; ES cell-associated protein 4; homeobox transcription factor Nanog; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein
UniProt Protein Name
Homeobox protein NANOG
UniProt Synonym Protein Names
ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog
Protein Family
UniProt Gene Name
Nanog  [Similar Products]
UniProt Synonym Gene Names
Ecat4; Enk  [Similar Products]
UniProt Entry Name
NANOG_MOUSE
NCBI Summary for Nanog
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
UniProt Comments for Nanog
NANOG: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Transcription factor; DNA-binding

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: response to retinoic acid; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; multicellular organismal development; stem cell division; endodermal cell fate specification; stem cell maintenance; stem cell differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; mesodermal cell fate commitment; negative regulation of BMP signaling pathway; negative regulation of cell fate commitment; response to organic substance; cell proliferation; embryonic development; negative regulation of cell differentiation; regulation of transcription, DNA-dependent; regulation of gene expression; regulation of cell differentiation; positive regulation of cell proliferation; negative regulation of endodermal cell fate specification; positive regulation of transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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