• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NCAN elisa kit :: Rat Neurocan (NCAN) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS450382
Unit / Price
  48-Strip-Wells  /  $425 +1 FREE 8GB USB
  96-Strip-Wells  /  $570 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,040 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Neurocan (NCAN), ELISA Kit

Popular Item
Full Product Name

Rat Neurocan (NCAN) ELISA Kit

Product Synonym Names
CSPG3; NEUR; Neurocan Core Protein; Chondroitin Sulfate Proteoglycan 3; Neurocan Proteoglycan
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P55067
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of NCAN. No significant cross-reactivity or interference between NCAN and analogues was observed.
Samples
Tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.055ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NCAN were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NCAN were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NCAN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNCANpurchase
MBS450382 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neurocan (NCAN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NCAN. The ELISA analytical biochemical technique of the MBS450382 kit is based on NCAN antibody-NCAN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NCAN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NCAN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NCAN elisa kit
Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NCAN in rat tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NCAN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NCAN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NCAN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NCAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Standard Curve/Testing Data of NCAN elisa kit
NCAN elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS450382. Click to request current manual
NCBI/Uniprot data below describe general gene information for NCAN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
135,545 Da
NCBI Official Full Name
Neurocan core protein
NCBI Official Synonym Full Names
neurocan
NCBI Official Symbol
NCBI Official Synonym Symbols
Cspg3
  [Similar Products]
NCBI Protein Information
neurocan core protein
UniProt Protein Name
Neurocan core protein
UniProt Synonym Protein Names
245 kDa early postnatal core glycoprotein; Chondroitin sulfate proteoglycan 3
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Cspg3  [Similar Products]
NCBI Summary for NCAN
chondroitin sulfate proteoglycan that may play a role in cell adhesion and cell migration [RGD, Feb 2006]
UniProt Comments for NCAN
May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with NCAN elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >96 publications with NCAN and Brain
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000