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NCL recombinant protein :: Nucleolin (NCL) Recombinant Protein

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Catalog # MBS2010481 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $145 +1 FREE 8GB USB
  0.05 mg  /  $280 +1 FREE 8GB USB
  0.1 mg  /  $440 +1 FREE 8GB USB
  0.2 mg  /  $540 +1 FREE 8GB USB
  0.5 mg  /  $1,040 +1 FREE 8GB USB
  1 mg  /  $1,545 +1 FREE 8GB USB
Product Name

Nucleolin (NCL), Recombinant Protein

Also Known As

Recombinant Nucleolin (NCL)

Matching Pairs
Matching Pairs
Biotin Conjugated Antibody: Nucleolin (NCL) (MBS2006419)
Immunogen: Nucleolin (NCL) (MBS2010481)
Matching Pairs
APC-CY7 Conjugated Antibody: Nucleolin (NCL) (MBS2064232)
Immunogen: Nucleolin (NCL) (MBS2010481)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
707
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF- YVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE GRTIRLELQG SNSRSQPS
Chromosome Location
Chromosome: 1; NC_000067.6 (86344719..86359455, complement). Location: 1 D; 1 43.94 cM
3D Structure
ModBase 3D Structure for P09405
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Tyr353~Ser568 (Accession # P09405) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of NCL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NCL recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for NCL recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Sequence Information of NCL recombinant protein
NCL recombinant protein Sequence Information image
NCBI/Uniprot data below describe general gene information for NCL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25.8kDa
NCBI Official Full Name
nucleolin
NCBI Official Synonym Full Names
nucleolin
NCBI Official Symbol
NCBI Official Synonym Symbols
C23; Nucl; D0Nds28; D1Nds28; B530004O11Rik
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NCBI Protein Information
nucleolin
UniProt Protein Name
Nucleolin
UniProt Synonym Protein Names
Protein C23
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
NUCL_MOUSE
UniProt Comments for NCL
NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.

Protein type: Helicase; RNA-binding; DNA-binding; Nuclear receptor co-regulator; Nucleolus

Cellular Component: nucleoplasm; cell surface; dense fibrillar component; membrane; cytoplasm; fibrillar center; nucleolus; cell cortex; nucleus; ribonucleoprotein complex

Molecular Function: protein C-terminus binding; identical protein binding; histone binding; RNA binding; nucleotide binding; laminin binding; telomeric DNA binding; calcium ion binding; protein binding; nucleic acid binding; rRNA primary transcript binding; DNA binding; sequence-specific DNA binding; single-stranded DNA binding; receptor binding

Biological Process: endocytosis; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of tumor necrosis factor production; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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