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NCOA3 recombinant protein :: Nuclear Receptor Coactivator 3 Recombinant Protein

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Catalog # MBS2031278 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $140 +1 FREE 8GB USB
  0.05 mg  /  $265 +1 FREE 8GB USB
  0.1 mg  /  $415 +1 FREE 8GB USB
  0.2 mg  /  $510 +1 FREE 8GB USB
  0.5 mg  /  $990 +1 FREE 8GB USB
  1 mg  /  $1,465 +1 FREE 8GB USB
SDS-Page
Product Name

Nuclear Receptor Coactivator 3 (NCOA3), Recombinant Protein

Popular Item
Full Product Name

Recombinant Nuclear Receptor Coactivator 3 (NCOA3)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Host
E.coli
Species Reactivity
Human
Purity/Purification
> 97%
Form/Format
100mM NaHCO3, 500mM NaCl, pH8.3, containing 0.01% SKL, 5% Trehalose and Proclin300.
Concentration
Original Concentration: 200ug/mL (lot specific)
Source
Prokaryotic expression
Residues
Gly1023~Ala1304
Tags
N-terminal His Tag
Subcellular Location
Nucleus, Cytoplasm
Traits
Freeze-dried powder
Predicted isoelectric point
7.2
Predicted Molecular Mass
35.3KDa
Accurate Molecular Mass
40kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of NCOA3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for NCOA3 recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB
(May be suitable for use in other assays to be determined by the end user.)

NCBI/Uniprot data below describe general gene information for NCOA3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
nuclear receptor coactivator 3
NCBI Official Synonym Full Names
nuclear receptor coactivator 3
NCBI Official Symbol
Ncoa3  [Similar Products]
NCBI Official Synonym Symbols
Actr; Aib1; Rac3; Src3; pCip; Tram1; p/Cip; KAT13B; Tram-1; bHLHe42; AW321064; 2010305B15Rik
  [Similar Products]
NCBI Protein Information
nuclear receptor coactivator 3
UniProt Protein Name
Nuclear receptor coactivator 3
UniProt Synonym Protein Names
Amplified in breast cancer-1 protein homolog; AIB-1; CBP-interacting protein; p/CIP; pCIP; Receptor-associated coactivator 3; RAC-3; Steroid receptor coactivator protein 3; SRC-3; Thyroid hormone receptor activator molecule 1; ACTR; TRAM-1
UniProt Gene Name
Ncoa3  [Similar Products]
UniProt Synonym Gene Names
Aib1; Pcip; Rac3; Tram1; NCoA-3; AIB-1; p/CIP; pCIP; RAC-3; SRC-3; ACTR; TRAM-1  [Similar Products]
UniProt Entry Name
NCOA3_MOUSE
UniProt Comments for NCOA3
SRC-3: a histone acetyltransferase and nuclear receptor coactivator. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Activity is enhanced by various hormones and TNF. Acetylation by CREBBP may suppress coactivation of target genes by disrupting its association with nuclear receptors. Frequently amplified or overexpressed in breast and ovarian cancers. Five alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 2.3.1.48; Transcription, coactivator/corepressor; Acetyltransferase; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; Golgi apparatus; perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; protein dimerization activity; peroxisome proliferator activated receptor binding; transcription coactivator activity; protein N-terminus binding; transferase activity; signal transducer activity; histone acetyltransferase activity; ligand-dependent nuclear receptor transcription coactivator activity; androgen receptor binding; transferase activity, transferring acyl groups; protein complex binding; estrogen receptor binding; chromatin binding; nuclear hormone receptor binding

Biological Process: developmental growth; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of steroid hormone receptor signaling pathway; multicellular organism growth; positive regulation of estrogen receptor signaling pathway; vagina development; signal transduction; positive regulation of cell growth; cellular response to hormone stimulus; regulation of transcription, DNA-dependent; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; histone acetylation; positive regulation of keratinocyte differentiation; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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