• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NE elisa kit :: Rat neutrophil elastase, NE ELISA Kit

Scan QR to view Datasheet
Catalog # MBS704356
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $575 +1 FREE 8GB USB
  96-Strip-Wells  /  $835 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,040 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,755 +7 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

neutrophil elastase, NE, ELISA Kit

Popular Item
Also Known As

Rat neutrophil elastase, NE ELISA Kit

Product Synonym Names
Rat neutrophil elastase; NE ELISA Kit; neutrophil elastase; NE
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS704356 Testing Data
Sequence Length
267
OMIM
202700
3D Structure
ModBase 3D Structure for P08246
Species Reactivity
Samples
Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates
Detection Range
12.5 ng/ml-800 ng/ml
Sensitivity
3.12 ng/ml
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNEpurchase
MBS704356 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the neutrophil elastase, NE, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NE. The ELISA analytical biochemical technique of the MBS704356 kit is based on NE antibody-NE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NE elisa kit
Introduction: Neutrophil elastase is a serine protease in the same family as chymotrypsin and has broad substrate specificity. Secreted by neutrophils during inflammation, it destroys bacteria and host tissue. As with other serine proteases it contains a charge relay system composed of the catalytic triad of histidine, aspartate, and serine residues that are dispersed throughout the primary sequence of the polypeptide but that are brought together in the three dimension conformation of the folded protein. The gene encoding neutrophil elastase consists of five exons. It is one of the two forms of elastase. The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate side chains and four intramolecular disulfide bridges. It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb. Neutrophil elastase is an important protease enzyme that when expressed aberrantly can cause emphysema or emphysematous changes. This involves breakdown of the lung structure and increased airspaces. Mutations of the NE gene cause severe congenital neutropenia, which is a failure of neutrophils to mature.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NE and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of NE elisa kit
NE elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704356. Click to request current manual
NCBI/Uniprot data below describe general gene information for NE. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,518 Da
NCBI Official Full Name
neutrophil elastase preproprotein
NCBI Official Synonym Full Names
elastase, neutrophil expressed
NCBI Official Symbol
ELANE  [Similar Products]
NCBI Official Synonym Symbols
GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
  [Similar Products]
NCBI Protein Information
neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase
UniProt Protein Name
Neutrophil elastase
UniProt Synonym Protein Names
Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase
UniProt Gene Name
ELANE  [Similar Products]
UniProt Synonym Gene Names
ELA2; HLE  [Similar Products]
UniProt Entry Name
ELNE_HUMAN
NCBI Summary for NE
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]
UniProt Comments for NE
Function: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.1 PublicationManual assertion based on experiment in:Ref.15

Catalytic activity: Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withNE elisa kit
 Reference Product  PubMed Publications
 MPO elisa kit  >2 publications with NE and MPO
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000