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NEDD8 recombinant protein

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Catalog # MBS203213
Unit / Price
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  0.1 mg  /  $310 +1 FREE 8GB USB
  0.5 mg  /  $720 +1 FREE 8GB USB
SDS-Page
Product Name

NEDD8, Recombinant Protein

Full Product Name

NEDD8, 1-76aa, Human, His tag, E Coli

Product Synonym Names
Neural precursor cell expressed developmentally down-regulated 8; ubiquitin-like protein Nedd8; Neddylin; Neural precursor cell expressed; developmentally down-regulated 8 MGC104393; MGC125896; MGC125897; NED8; 0NEDD 8; Neddylin; Neural precursor cell expressed developmentally down regulated 8; Neural precursor cell expressed developmentally down regulated gene 8; Rub1; ubiquitin like protein Nedd 8; ubiquitin like protein Nedd8.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE KTAADYKILG GSVLHLVLAL RGG
OMIM
D23662 mRNA
3D Structure
ModBase 3D Structure for Q15843
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.5mM DTT, 50mM NaCl, 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of NEDD8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NEDD8 recombinant protein
NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E Coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.
Product Categories/Family for NEDD8 recombinant protein
Applications Tested/Suitable for NEDD8 recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for NEDD8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
12.8 kDa (113 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
NEDD8
NCBI Official Synonym Full Names
neural precursor cell expressed, developmentally down-regulated 8
NCBI Official Symbol
NEDD8  [Similar Products]
NCBI Official Synonym Symbols
NEDD-8
  [Similar Products]
NCBI Protein Information
NEDD8
UniProt Protein Name
NEDD8
UniProt Synonym Protein Names
Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8
Protein Family
UniProt Gene Name
NEDD8  [Similar Products]
UniProt Synonym Gene Names
NEDD-8  [Similar Products]
UniProt Comments for NEDD8
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Product References and Citations for NEDD8 recombinant protein
Wu K., et al. (2002) J Biol Chem. 277 (1): 516-27; Hori T., et al. (1999) Oncogene. 18 (48): 6829-34;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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