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NEFH elisa kit :: Pig Neurofilament heavy polypeptide ELISA Kit

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Catalog # MBS289548
Unit / Price
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  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Neurofilament heavy polypeptide (NEFH), ELISA Kit

Popular Item
Full Product Name

Pig Neurofilament heavy polypeptide ELISA Kit

Product Synonym Names
Neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein; NEFH
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P12037
Species Reactivity
Specificity
This assay recognizes recombinant and natural pigNF-H. No significant cross-reactivity or interference was observed.
Samples
Serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Detection Range
15.6pg/mL -1000pg/mL.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NEFH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for NEFHpurchase
MBS289548 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neurofilament heavy polypeptide (NEFH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NEFH. The ELISA analytical biochemical technique of the MBS289548 kit is based on NEFH antibody-NEFH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NEFH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NEFH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NEFH elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofpigNF-Hconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to NF-H. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forNF-H and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NF-H in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve of NEFH elisa kit
NEFH elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS289548. Click to request current manual
NCBI/Uniprot data below describe general gene information for NEFH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
16,204 Da
NCBI Official Full Name
Neurofilament heavy polypeptide
UniProt Protein Name
Neurofilament heavy polypeptide
UniProt Synonym Protein Names
200 kDa neurofilament protein; Neurofilament triplet H protein
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
NFH_PIG
UniProt Comments for NEFH
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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