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NEFL elisa kit :: Pig neurofilament, light polypeptide ELISA Kit

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Catalog # MBS919592
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Typical Testing Data/Standard Curve (for reference only)
Product Name

neurofilament, light polypeptide (NEFL), ELISA Kit

Popular Item
Also Known As

Pig Neurofilament light polypeptide, NEFL ELISA Kit

Product Synonym Names
Pig Neurofilament light polypeptide (NEFL) ELISA kit; CMT1F; CMT2E; FLJ53642; NF-L; NF68; NFL; light molecular weight neurofilament protein; neurofilament protein; light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament; lig; neurofilament; light polypeptide
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS919592 Testing Data
Sequence Length
3D Structure
ModBase 3D Structure for P02547
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of Pig NEFL. No significant cross-reactivity or interference between Pig NEFL and analogues was observed.
Assay Type
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NEFL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNEFLpurchase
MBS919592 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the neurofilament, light polypeptide (NEFL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NEFL. The ELISA analytical biochemical technique of the MBS919592 kit is based on NEFL antibody-NEFL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NEFL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NEFL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NEFL elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NEFL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NEFL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NEFL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NEFL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NEFL elisa kit
NEFL elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS919592. Click to request current manual
NCBI/Uniprot data below describe general gene information for NEFL. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
62,072 Da
NCBI Official Full Name
Neurofilament light polypeptide
UniProt Protein Name
Neurofilament light polypeptide
UniProt Synonym Protein Names
68 kDa neurofilament protein; Neurofilament triplet L protein
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
UniProt Comments for NEFL
NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).

Protein type: Cytoskeletal
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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