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NEGR1 elisa kit :: Human neuronal growth regulator 1 ELISA Kit

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Catalog # MBS919685
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Typical Testing Data/Standard Curve (for reference only)
Product Name

neuronal growth regulator 1 (NEGR1), ELISA Kit

Popular Item
Full Product Name

Human Neuronal growth regulator 1, NEGR1 ELISA Kit

Product Synonym Names
Human Neuronal growth regulator 1 (NEGR1) ELISA kit; UNQ2433; DMML2433; IGLON4; KILON; MGC46680; Ntra; IgLON family member 4; a kindred of IgLON; neurotractin; neuronal growth regulator 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS919685 Testing Data
OMIM
613173
3D Structure
ModBase 3D Structure for Q7Z3B1
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human NEGR1. No significant cross-reactivity or interference between Human NEGR1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
18.75 pg/ml-1200 pg/ml
Sensitivity
4.69 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Cell Adhesion
Protein Biological Process 3
Cell adhesion
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NEGR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for NEGR1purchase
MBS919685 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the neuronal growth regulator 1 (NEGR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NEGR1. The ELISA analytical biochemical technique of the MBS919685 kit is based on NEGR1 antibody-NEGR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NEGR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NEGR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NEGR1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NEGR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NEGR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NEGR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NEGR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NEGR1 elisa kit
NEGR1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS919685. Click to request current manual
NCBI/Uniprot data below describe general gene information for NEGR1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,719 Da
NCBI Official Full Name
neuronal growth regulator 1
NCBI Official Synonym Full Names
neuronal growth regulator 1
NCBI Official Symbol
NEGR1  [Similar Products]
NCBI Official Synonym Symbols
Ntra; KILON; IGLON4; DMML2433
  [Similar Products]
NCBI Protein Information
neuronal growth regulator 1; neurotractin; a kindred of IgLON; IgLON family member 4
UniProt Protein Name
Neuronal growth regulator 1
UniProt Synonym Protein Names
IgLON family member 4
Protein Family
UniProt Gene Name
NEGR1  [Similar Products]
UniProt Synonym Gene Names
IGLON4; UNQ2433/PRO4993  [Similar Products]
UniProt Entry Name
NEGR1_HUMAN
UniProt Comments for NEGR1
NEGR1: May be involved in cell-adhesion. May function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain. Belongs to the immunoglobulin superfamily. IgLON family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: neuron projection; cell surface; cell soma; plasma membrane; intercellular junction

Molecular Function: protein binding

Biological Process: cell-cell adhesion; regulation of neurogenesis; locomotory behavior; feeding behavior
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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