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NELL1 elisa kit :: Human NEL-like 1 (chicken) ELISA Kit

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Catalog # MBS900586
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
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  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

NEL-like 1 (chicken) (NELL1), ELISA Kit

Popular Item
Full Product Name

Human Protein kinase C-binding protein NELL1, NELL1 ELISA Kit

Product Synonym Names
Human Protein kinase C-binding protein NELL1 (NELL1) ELISA kit; FLJ45906; IDH3GL; NRP1; isocitrate dehydrogenase 3 (NAD+) gamma-like; nel-like 1; neural epidermal growth factor-like 1; NEL-like 1 (chicken)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602319
3D Structure
ModBase 3D Structure for Q92832
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human NELL1. No significant cross-reactivity or interference between human NELL1 and analogues was observed.
Samples
Serum, plasma, cell culture supernates, tissue homogenates.
Assay Type
Sandwich
Detection Range
31.25 pg/ml -2000 pg/ml.
Sensitivity
7.81 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NELL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for NELL1purchase
MBS900586 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NEL-like 1 (chicken) (NELL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NELL1. The ELISA analytical biochemical technique of the MBS900586 kit is based on NELL1 antibody-NELL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NELL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NELL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NELL1 elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NELL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NELL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NELL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NELL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NELL1 elisa kit
NELL1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for NELL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
89,635 Da
NCBI Official Full Name
protein kinase C-binding protein NELL1 isoform 3
NCBI Official Synonym Full Names
NEL-like 1 (chicken)
NCBI Official Symbol
NELL1  [Similar Products]
NCBI Official Synonym Symbols
NRP1; IDH3GL
  [Similar Products]
NCBI Protein Information
protein kinase C-binding protein NELL1; nel-related protein 1; neural epidermal growth factor-like 1
UniProt Protein Name
Protein kinase C-binding protein NELL1
UniProt Synonym Protein Names
NEL-like protein 1; Nel-related protein 1
UniProt Gene Name
NELL1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
NELL1_HUMAN
NCBI Summary for NELL1
This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
UniProt Comments for NELL1
NELL1: Plays a role in the control of cell growth and differentiation. Promotes osteoblast cell differentiation and terminal mineralization. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: perinuclear region of cytoplasm; cytoplasm; extracellular region; nuclear envelope

Molecular Function: protein binding; calcium ion binding

Biological Process: positive regulation of osteoblast differentiation; nervous system development; regulation of gene expression; negative regulation of osteoblast proliferation; cell differentiation; positive regulation of bone mineralization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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