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anti-Neuropilin-1 (pT916) antibody :: Rabbit Neuropilin-1 (pT916) Antibody

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Catalog # MBS462092
Unit / Price
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  0.1 mg  /  $285 +1 FREE 8GB USB
Testing Data
Product Name

Neuropilin-1 (pT916), Antibody

Popular Item
Full Product Name

Rabbit anti Neuropilin 1 (pThr916), Phosphospecific

Product Synonym Names
Rabbit anti-Neuropilin1 (pThr916); CD304 antigen; NRP1; VEGF Receptor
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 10; NC_000010.10 (33466419..33623833, complement). Location: 10p12
OMIM
602069
3D Structure
ModBase 3D Structure for O14786
Host
Rabbit
Species Reactivity
Human, Bovine
Specificity
This antibody recognizes ~130 kDa of human Neuropilin1 protein at the phosphorylation site of Thr 916. It does not cross react to non-phospho Neuropilin 1. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (lot specific)
Antigen Preparation
A synthetic peptide surrounding the epitope -LNTQS- with a phosphorylation site at Thr916. This sequence is identical among human and bovine.
Positive Control
Human Brain Tissue
Cellular Location
Cytoplasmic/membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C -8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-Neuropilin-1 (pT916) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Neuropilin-1 (pT916) antibody
The neuropilin-1 (Nrp1) is a multifunctional protein, identified principally as a receptor for the class 3 semaphorins and members of the vascular endothelial growth factor (VEGF) family, but it is capable of other interactions. It is a marker of regulatory T cells (Tr), which often carry Nrp1 and latency-associated peptide (LAP)-TGF-beta1 (the latent form). Nrp1 is reported to be expressed in breast cancer cells. The ischemia can increase neuropilin 1 protein expression in experimental rat brain as well. The phosphorylation of Nrp1 is essential for the Nrp1 activation.
Applications Tested/Suitable for anti-Neuropilin-1 (pT916) antibody
ELISA, Western Blot, Immunohistochemistry
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:200
Optimal dilutions should be determined by researchers for the specific applications.

NCBI/Uniprot data below describe general gene information for Neuropilin-1 (pT916). It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
130 kDa
NCBI Official Full Name
neuropilin-1 isoform a
NCBI Official Synonym Full Names
neuropilin 1
NCBI Official Symbol
NCBI Official Synonym Symbols
NP1; NRP; BDCA4; CD304; VEGF165R; DKFZp781F1414; DKFZp686A03134
  [Similar Products]
NCBI Protein Information
neuropilin-1; OTTHUMP00000020818; OTTHUMP00000020819; OTTHUMP00000020820; OTTHUMP00000020821; OTTHUMP00000020822; transmembrane receptor; vascular endothelial cell growth factor 165 receptor
UniProt Protein Name
Neuropilin-1
UniProt Synonym Protein Names
Vascular endothelial cell growth factor 165 receptor
UniProt Gene Name
UniProt Synonym Gene Names
NRP; VEGF165R  [Similar Products]
UniProt Entry Name
NRP1_HUMAN
NCBI Summary for Neuropilin-1 (pT916)
NRP1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF; MIM 192240) and semaphorin (see SEMA3A; MIM 603961) family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.[supplied by OMIM]
UniProt Comments for Neuropilin-1 (pT916)
Function: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.

Subunit structure: Homodimer, and heterodimer with NRP2. Binds PLXNB1. Ref.17

Subcellular location: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Tissue specificity: The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.

Domain: The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding.

Sequence similarities: Belongs to the neuropilin family.Contains 2 CUB domains.Contains 2 F5/8 type C domains.Contains 1 MAM domain.
Product References and Citations for anti-Neuropilin-1 (pT916) antibody
Eldar Hochman et al. Molecular Pathways Regulating Pro-migratory Effects of Hedgehog Signaling. J. Biol. Chem., Vol. 281, Issue 45, 33860-33870, November 10, 2006 Glinka Y, et al. Neuropilin-1 is a receptor for transforming growth factor beta-1, activates its latent form, and promotes regulatory T cell activity. J Leukoc Biol. 2008 Jul; 84 (1): 302-10. Epub 2008 Apr 24

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-Neuropilin-1 (pT916) antibody
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >11 publications with Neuropilin-1 (pT916) and Muscle
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