• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NF-kappaB p65 elisa kit :: Mouse Nuclear Factor-kappaB p65 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS762054
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Nuclear Factor-kappaB p65 (NF-kappaB p65), ELISA Kit

Popular Item
Also Known As

Mouse Nuclear Factor-kappaB p65 ELISA Kit

Product Synonym Names
NF-kappaB p65/NFkB p65/NFKB3/p65RelA/MGC131774/NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))/Nuclear factor NF-kappa-B p65 subunit/Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65/p65/v-rel reticuloendotheliosis viral oncogene homolog A (avian)/v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappalight polypeptide gene enhancer in B-cells 3; p65; Nuclear Factor-kB p65
Product Synonym Gene Name
NF-kB p65[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS762054 COA
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of NF-kappaB p65. No significant cross-reactivity or interference between NF-kappaB p65 and analogues was observed.
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NF- kappaB p65 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NF- kappaB p65 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NF-kappaB p65 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNF-kappaB p65purchase
MBS762054 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nuclear Factor-kappaB p65 (NF-kappaB p65) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NF-kappaB p65. The ELISA analytical biochemical technique of the MBS762054 kit is based on NF-kappaB p65 antibody-NF-kappaB p65 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NF-kappaB p65 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NF-kappaB p65. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NF-kappaB p65 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NF-kappaB p65 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NF-kappaB p65 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NF-kappaB p65 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NF-kappaB p65 can be calculated.

Typical Standard Curve/Testing Data of NF-kappaB p65 elisa kit
NF-kappaB p65 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS762054. Click to request current manual
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

Contact Us

Please fill out the form below and our representative will get back to you shortly.