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NFATC1 elisa kit :: Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 ELISA Kit

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Catalog # MBS920738
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Typical Testing Data/Standard Curve (for reference only)
Product Name

nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATC1), ELISA Kit

Popular Item
Full Product Name

Human Nuclear factor of activated T-cells, cytoplasmic 1, NFATC1 ELISA Kit

Product Synonym Names
Human Nuclear factor of activated T-cells; cytoplasmic 1 (NFATC1) ELISA kit; MGC138448; NF-ATC; NFAT2; NFATc; NFAT transcription complex cytosolic component; nuclear factor of activated T-cells; cytosolic component 1; nuclear factor of activated T-cells; cytoplasmic; calcineurin-dependent 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS920738 Testing Data
OMIM
600489
3D Structure
ModBase 3D Structure for O95644
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human NFATC1. No significant cross-reactivity or interference between human NFATC1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates.
Assay Type
Sandwich
Detection Range
25 pg/ml -1600 pg/ml.
Sensitivity
6.25 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NFATC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNFATC1purchase
MBS920738 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NFATC1. The ELISA analytical biochemical technique of the MBS920738 kit is based on NFATC1 antibody-NFATC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NFATC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NFATC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NFATC1 elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NFATC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NFATC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NFATC1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NFATC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NFATC1 elisa kit
NFATC1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS920738. Click to request current manual
NCBI/Uniprot data below describe general gene information for NFATC1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
101,243 Da
NCBI Official Full Name
nuclear factor of activated T-cells, cytoplasmic 1 isoform F
NCBI Official Synonym Full Names
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
NCBI Official Symbol
NFATC1  [Similar Products]
NCBI Official Synonym Symbols
NFAT2; NFATc; NF-ATC
  [Similar Products]
NCBI Protein Information
nuclear factor of activated T-cells, cytoplasmic 1; NFAT transcription complex cytosolic component
UniProt Protein Name
Nuclear factor of activated T-cells, cytoplasmic 1
UniProt Synonym Protein Names
NFAT transcription complex cytosolic component; NF-ATc; NFATc
UniProt Gene Name
NFATC1  [Similar Products]
UniProt Synonym Gene Names
NFAT2; NFATC; NF-ATc1; NFATc1; NF-ATc; NFATc  [Similar Products]
UniProt Entry Name
NFAC1_HUMAN
NCBI Summary for NFATC1
The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]
UniProt Comments for NFATC1
NFAT2: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Also control gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of lymphoid and nonlymphoid cells. Six splice variant isoforms have been identified. Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; FK506 binding; mitogen-activated protein kinase p38 binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; calcium ion transport; positive regulation of transcription, DNA-dependent; heart development; innate immune response; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; osteoclast differentiation; G1/S transition of mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with NFATC1 elisa kit
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