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NFE2L2 elisa kit :: Human Nuclear factor erythroid 2-related factor 2 ELISA Kit

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Catalog # MBS289787
Unit / Price
  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Nuclear factor erythroid 2-related factor 2 (NFE2L2), ELISA Kit

Popular Item
Also Known As

Human Nuclear factor erythroid 2-related factor 2 ELISA Kit

Product Synonym Names
Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor, erythroid derived 2, like 2; NFE2L2; NRF2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS289787 Testing Data
Sequence Length
589
OMIM
600492
3D Structure
ModBase 3D Structure for Q16236
Species Reactivity
Assay Type
Sandwich
Detection Range
0.312-20 ng/mL
Sensitivity
0.184 ng/mL
Intra-Assay CV
<=8.1%
Inter-Assay CV
<=10.2%
Recovery
99%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NFE2L2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNFE2L2purchase
MBS289787 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nuclear factor erythroid 2-related factor 2 (NFE2L2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NFE2L2. The ELISA analytical biochemical technique of the MBS289787 kit is based on NFE2L2 antibody-NFE2L2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NFE2L2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NFE2L2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NFE2L2 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of NFE2L2 elisa kit
NFE2L2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS289787. Click to request current manual
NCBI/Uniprot data below describe general gene information for NFE2L2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
65,356 Da
NCBI Official Full Name
nuclear factor erythroid 2-related factor 2 isoform 2
NCBI Official Synonym Full Names
nuclear factor, erythroid 2 like 2
NCBI Official Symbol
NFE2L2  [Similar Products]
NCBI Official Synonym Symbols
NRF2; HEBP1
  [Similar Products]
NCBI Protein Information
nuclear factor erythroid 2-related factor 2
UniProt Protein Name
Nuclear factor erythroid 2-related factor 2
UniProt Synonym Protein Names
HEBP1; Nuclear factor, erythroid derived 2, like 2
UniProt Gene Name
NFE2L2  [Similar Products]
UniProt Synonym Gene Names
NRF2; NF-E2-related factor 2; NFE2-related factor 2  [Similar Products]
UniProt Entry Name
NF2L2_HUMAN
NCBI Summary for NFE2L2
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]
UniProt Comments for NFE2L2
NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: centrosome; chromatin; cytoplasm; cytosol; nucleus; plasma membrane

Molecular Function: DNA binding; protein binding; protein domain specific binding; transcription factor activity

Biological Process: cell redox homeostasis; cellular response to glucose starvation; inflammatory response; positive regulation of blood coagulation; positive regulation of glucose import; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; regulation of embryonic development; transcription from RNA polymerase II promoter; unfolded protein response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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