• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NFkappaB p65 elisa kit :: Rat nuclear factor-B p65, NF-kappaB p65 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS704916
Unit / Price
Scan QR to view Datasheet
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $590 +1 FREE 8GB USB
  96-Strip-Wells  /  $855 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,110 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,890 +7 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

nuclear factor-B p65, NF-kappaB p65 (NFkappaB p65), ELISA Kit

Popular Item
Full Product Name

Rat nuclear factor-B p65, NF-kappaB p65 ELISA Kit

Product Synonym Names
Rat nuclear factor-kappaB p65; NF-kappaB p65 ELISA Kit; nuclear factor-kappaB p65; NF-kappaB p65
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat NF-kB p65. No significant cross-reactivity or interference between rat NF-kB p65 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
1.56 pg/ml -100 pg/ml.
Sensitivity
The minimum detectable dose of rat NF-kB p65 is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NFkappaB p65 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for NFkappaB p65purchase
MBS704916 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nuclear factor-B p65, NF-kappaB p65 (NFkappaB p65) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NFkappaB p65. The ELISA analytical biochemical technique of the MBS704916 kit is based on NFkappaB p65 antibody-NFkappaB p65 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NFkappaB p65 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NFkappaB p65. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NFkappaB p65 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-kappaB p65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-kappaB p65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-kappaB p65 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-kappaB p65 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NFkappaB p65 elisa kit
NFkappaB p65 elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for NFkappaB p65. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
NFkappaB p65
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000