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NGF elisa kit :: Human Nerve Growth Factor ELISA Kit

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Catalog # MBS732618
Unit / Price
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  48-Strip-Wells-(Competitive)  /  $440 +1 FREE 8GB USB
  48-Strip-Wells-(Sandwich)  /  $440 +1 FREE 8GB USB
  96-Strip-Wells-(Competitive)  /  $640 +1 FREE 8GB USB
  96-Strip-Wells-(Sandwich)  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells-(Competitive)  /  $2,895 +3 FREE 8GB USB
  5x96-Strip-Wells-(Sandwich)  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells-(Competitive)  /  $5,415 +6 FREE 8GB USB
  10x96-Strip-Wells-(Sandwich)  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Nerve Growth Factor (NGF), ELISA Kit

Full Product Name

Human Nerve Growth Factor ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 608654
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Highest Standards
10ng/mL
Concentration of Standards
Concentration of Standards
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNGFpurchase
MBS732618 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nerve Growth Factor (NGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NGF. The ELISA analytical biochemical technique of the MBS732618 kit is based on NGF antibody-NGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for NGF elisa kit

Typical Testing Data/Standard Curve (for reference only) of NGF elisa kit
NGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS732618. Click to request current manual
NCBI/Uniprot data below describe general gene information for NGF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
26,959 Da
NCBI Official Full Name
NGF protein, partial
NCBI Official Synonym Full Names
nerve growth factor (beta polypeptide)
NCBI Official Symbol
NCBI Official Synonym Symbols
NGFB; HSAN5; Beta-NGF
  [Similar Products]
NCBI Protein Information
beta-nerve growth factor; nerve growth factor, beta subunit
UniProt Protein Name
Beta-nerve growth factor
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NGFB; Beta-NGF  [Similar Products]
UniProt Entry Name
NGF_HUMAN
NCBI Summary for NGF
This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]
UniProt Comments for NGF
NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: extracellular space; Golgi lumen; cytoplasmic membrane-bound vesicle; extracellular region; endosome

Molecular Function: metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; protein binding; growth factor activity; receptor signaling protein activity

Biological Process: circadian rhythm; response to nicotine; response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; activation of MAPKK activity; adult locomotory behavior; positive regulation of apoptosis; regulation of neuron differentiation; positive regulation of axon extension; response to glucocorticoid stimulus; regulation of neurotransmitter secretion; response to lipopolysaccharide; regulation of caspase activity; regulation of axonogenesis; sensory perception of pain; negative regulation of cell cycle; nerve growth factor processing; response to radiation; cell-cell signaling; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; inflammatory response; response to electrical stimulus; response to drug; positive regulation of neuron maturation; phosphoinositide-mediated signaling; positive regulation of protein amino acid autophosphorylation; positive regulation of nerve growth factor receptor signaling pathway; regulation of release of sequestered calcium ion into cytosol; memory; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; phospholipase C activation; response to mechanical stimulus; response to ozone; Ras protein signal transduction; positive regulation of transcription factor activity; positive regulation of axonogenesis; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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