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NGF elisa kit :: Rat NGF/NGF beta ELISA Kit

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Catalog # MBS175864
Unit / Price
  96-Strip-Wells  /  $360 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

NGF/NGF beta, ELISA Kit

Popular Item
Full Product Name

Rat NGF/NGF beta PicoKine ELISA Kit

Product Synonym Names
Beta-nerve growth factor; nerve growth factor (beta polypeptide); Beta nerve growth factor; Beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor (beta polypeptide); Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGFB; NID67
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P25427
Species Reactivity
Specificity
Natural and recombinant rat NGF
Samples
Cell culture supernates and serum.
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNGFpurchase
MBS175864 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the NGF/NGF beta, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NGF. The ELISA analytical biochemical technique of the MBS175864 kit is based on NGF antibody-NGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NGF elisa kit
Background/Introduction: Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF.1 NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.2

Principle of the Assay: The rat NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NGF has been precoated onto 96-well plates. Standards(NSO, S122-G241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat NGF amount of sample captured in plate.

Typical Testing Data/Standard Curve (for reference only) of NGF elisa kit
Rat NGF/NGF beta PicoKine ELISA Kit standard curve
NGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175864. Click to request current manual
NCBI/Uniprot data below describe general gene information for NGF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
27,010 Da
NCBI Official Full Name
Beta-nerve growth factor
NCBI Official Synonym Full Names
nerve growth factor (beta polypeptide)
NCBI Official Symbol
NCBI Official Synonym Symbols
Ngfb; beta-NGF
  [Similar Products]
NCBI Protein Information
beta-nerve growth factor
UniProt Protein Name
Beta-nerve growth factor
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Ngfb; Beta-NGF  [Similar Products]
UniProt Entry Name
NGF_RAT
UniProt Comments for NGF
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with NGF elisa kit
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