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NGLY1 elisa kit :: Human N-glycanase 1 ELISA Kit

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Catalog # MBS916030
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Typical Testing Data/Standard Curve (for reference only)
Product Name

N-glycanase 1 (NGLY1), ELISA Kit

Full Product Name

Human Peptide-N, NGLY1 ELISA Kit

Product Synonym Names
Human Peptide-N (NGLY1) ELISA kit; FLJ11005; FLJ12409; PNG1; PNGase; peptide-N (4) - (N-acetyl-beta-glucosaminyl) asparagine amidase; N-glycanase 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS916030 Testing Data
OMIM
gene 615273
3D Structure
ModBase 3D Structure for Q96IV0
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human NGLY1. No significant cross-reactivity or interference between human NGLY1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
37.5 pg/ml -2400 pg/ml.
Sensitivity
The minimum detectable dose of human NGLY1 is typically less than 9.38 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NGLY1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for NGLY1purchase
MBS916030 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the N-glycanase 1 (NGLY1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NGLY1. The ELISA analytical biochemical technique of the MBS916030 kit is based on NGLY1 antibody-NGLY1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NGLY1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NGLY1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NGLY1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGLY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGLY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGLY1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGLY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NGLY1 elisa kit
NGLY1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS916030. Click to request current manual
NCBI/Uniprot data below describe general gene information for NGLY1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
74,390 Da
NCBI Official Full Name
peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase isoform 2
NCBI Official Synonym Full Names
N-glycanase 1
NCBI Official Symbol
NGLY1  [Similar Products]
NCBI Official Synonym Symbols
PNG1; CDG1V; PNGase
  [Similar Products]
NCBI Protein Information
peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; hPNGase; peptide:N-glycanase
UniProt Protein Name
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
UniProt Synonym Protein Names
N-glycanase 1; Peptide:N-glycanase
UniProt Gene Name
NGLY1  [Similar Products]
UniProt Synonym Gene Names
PNG1; PNGase; hPNGase  [Similar Products]
UniProt Entry Name
NGLY1_HUMAN
NCBI Summary for NGLY1
This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009]
UniProt Comments for NGLY1
NGLY1: Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. Belongs to the transglutaminase-like superfamily. PNGase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.5.1.52

Chromosomal Location of Human Ortholog: 3p24.2

Cellular Component: cytoplasm

Molecular Function: protein binding; peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity; metal ion binding

Biological Process: glycoprotein catabolic process

Disease: Congenital Disorder Of Deglycosylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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