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NID1 elisa kit :: Human nidogen 1 ELISA Kit

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Catalog # MBS704872 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

nidogen 1 (NID1), ELISA Kit

Popular Item
Full Product Name

Human NiDogen 1, NID1 ELISA Kit

Product Synonym Names
Human Nidogen 1 (NID1) ELISA Kit; NID; enactin; entactin; nidogen 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS704872 Testing Data
OMIM
131390
3D Structure
ModBase 3D Structure for P14543
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human NID 1. No significant cross-reactivity or interference between human NID 1 and analogues was observed.
Samples
Serum, plasma, cell culture supernates
Assay Type
Sandwich
Detection Range
0.312 ng/ml -20 ng/ml.
Sensitivity
The minimum detectable dose of human NID 1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NID1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for NID1purchase
MBS704872 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the nidogen 1 (NID1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NID1. The ELISA analytical biochemical technique of the MBS704872 kit is based on NID1 antibody-NID1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NID1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NID1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NID1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NID 1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NID 1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NID 1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NID 1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of NID1 elisa kit
NID1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704872. Click to request current manual
NCBI/Uniprot data below describe general gene information for NID1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
136,377 Da
NCBI Official Full Name
nidogen-1
NCBI Official Synonym Full Names
nidogen 1
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
nidogen-1; NID-1; enactin; entactin
UniProt Protein Name
Nidogen-1
UniProt Synonym Protein Names
Entactin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NID; NID-1  [Similar Products]
UniProt Entry Name
NID1_HUMAN
NCBI Summary for NID1
This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008]
UniProt Comments for NID1
Function: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with NID1 elisa kit
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