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Nid1 recombinant protein :: Nidogen-1 Recombinant Protein

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Catalog # MBS954331
Unit / Price
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  0.01 mg (E-Coli)  /  $200 +1 FREE 8GB USB
  0.01 mg (Yeast)  /  $230 +1 FREE 8GB USB
  0.05 mg (E-Coli)  /  $260 +1 FREE 8GB USB
  0.05 mg (Yeast)  /  $305 +1 FREE 8GB USB
  0.1 mg (E-Coli)  /  $430 +1 FREE 8GB USB
  0.1 mg (Yeast)  /  $510 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $685 +1 FREE 8GB USB
  0.2 mg (Yeast)  /  $815 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $1,125 +1 FREE 8GB USB
  0.5 mg (Yeast)  /  $1,345 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,725 +2 FREE 8GB USB
  1 mg (Yeast)  /  $2,065 +2 FREE 8GB USB
SDS-Page
Product Name

Nidogen-1 (Nid1), Recombinant Protein

Popular Item
Full Product Name

Recombinant Mouse Nidogen-1

Product Synonym Names
Entactin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS954331 COA
Sequence Positions
428-665aa; Partial
Sequence
SPQRVNGKVK GRIFVGSSQV PVVFENTDLH SYVVMNHGRS YTAISTIPET VGYSLLPLAP IGGIIGWMFA VEQDGFKNGF SITGGEFTRQ AEVTFLGHPG KLVLKQQFSG IDEHGHLTIS TELEGRVPQI PYGASVHIEP YTELYHYSSS VITSSSTREY TVMEPDQDGA APSHTHIYQW RQTITFQECA HDDARPALPS TQQLSVDSVF VLYNKEERIL RYALSNSIGP VRDGSPDA
3D Structure
ModBase 3D Structure for P10493
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Production Note
Special Offer: The yeast host-expressed protein is manufactured from a stock plasmid containing the protein gene. Yeast host-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The yeast host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select yeast host-expressed protein for the fastest delivery among all hosts. Please contact us or email to support@mybiosource.com for more details.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of Nid1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Nid1 recombinant protein
Sulfated glycoprotein widely distributed in basent membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Nid1 recombinant protein SDS-Page image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for Nid1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30.3kD
NCBI Official Full Name
nidogen-1
NCBI Official Synonym Full Names
nidogen 1
NCBI Official Symbol
NCBI Official Synonym Symbols
Nid; entactin; nidogen-1; A630025O17; entactin-1
  [Similar Products]
NCBI Protein Information
nidogen-1
UniProt Protein Name
Nidogen-1
UniProt Synonym Protein Names
Entactin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Ent; NID-1  [Similar Products]
UniProt Entry Name
NID1_MOUSE
UniProt Comments for Nid1
Nidogen-1: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: basal lamina; basement membrane; extracellular matrix; extracellular region; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; collagen binding; extracellular matrix binding; laminin binding; laminin-1 binding; protein binding; proteoglycan binding

Biological Process: cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; glomerular basement membrane development
Product References and Citations for Nid1 recombinant protein
Amino acid sequence and domain structure of entactin. Homology with epidermal growth factor precursor and low density lipoprotein receptor.Durkin M.E., Chakravarti S., Bartos B.B., Liu S.H., Friedman R.L., Chung A.E.J. Cell Biol. 107:2749-2756(1988) Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells.Mann K., Deutzmann R., Aumailley M., Timpl R., Raimondi L., Yamamda Y., Pan T.-C., Conway D., Chu M.-L.EMBO J. 8:65-72(1989) Characterization of the 5' end of the mouse Ent gene encoding the basement membrane protein, entactin.Durkin M.E., Liu S.H., Reing J., Chung A.E.Gene 132:261-266(1993) Exon organization of the mouse entactin gene corresponds to the structural domains of the polypeptide and has regional homology to the low-density lipoprotein receptor gene.Durkin M.E., Wewer U.M., Chung A.E.Genomics 26:219-228(1995) Purification and structural characterization of intact and fragmented nidogen obtained from a tumor basement membrane.Paulsson M., Deutzmann R., Dziadek M., Nowack H., Timpl R., Weber S., Engel J.Eur. J. Biochem. 156:467-478(1986) Structure and localization of O- and N-linked oligosaccharide chains on basement membrane protein nidogen.Fujiwara S., Shinkai H., Mann K., Timpl R.Matrix 13:215-222(1993) Binding of fibulin-1 to nidogen depends on its C-terminal globular domain and a specific array of calcium-binding epidermal growth factor-like (EG) modules.Adam S., Goehring W., Wiedemann H., Chu M.-L., Timpl R., Kostka G.J. Mol. Biol. 272:226-236(1997) Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin.Sasaki T., Brakebusch C., Engel J., Timpl R.EMBO J. 17:1606-1613(1998) Recombinant domains of mouse nidogen-1 and their binding to basement membrane proteins and monoclonal antibodies.Ries A., Goehring W., Fox J.W., Timpl R., Sasaki T.Eur. J. Biochem. 268:5119-5128(2001) Identification of the basement membrane protein nidogen as a candidate ligand for tumor endothelial marker 7 in vitro and in vivo.Lee H.K., Seo I.A., Park H.K., Park H.T.FEBS Lett. 580:2253-2257(2006) Crystal structure and mutational analysis of a perlecan-binding fragment of nidogen-1.Hopf M., Gohring W., Ries A., Timpl R., Hohenester E.Nat. Struct. Biol. 8:634-640(2001)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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