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NIS recombinant protein :: Sodium Iodide Symporter Recombinant Protein

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Catalog # MBS2031325 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $135 +1 FREE 8GB USB
  0.05 mg  /  $250 +1 FREE 8GB USB
  0.1 mg  /  $385 +1 FREE 8GB USB
  0.2 mg  /  $470 +1 FREE 8GB USB
  0.5 mg  /  $910 +1 FREE 8GB USB
  1 mg  /  $1,345 +1 FREE 8GB USB
SDS-Page
Product Name

Sodium Iodide Symporter (NIS), Recombinant Protein

Popular Item
Also Known As

Recombinant Sodium Iodide Symporter (NIS)

Matching Pairs
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Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
274400
Host
E. coli
Species Reactivity
Human
Purity/Purification
>92%
Form/Format
100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
Concentration
Original Concentration: 200µg/mL (lot specific)
Source
Prokaryotic expression
Residues
Ser547~Leu643
Tags
Two N-terminal Tags, His-tag and GST-tag
Tissue Specificity
Thyroid, Ovary
Subcellular Location
Membrane; Multi-pass membrane protein
Traits
Freeze-dried powder
Predicted isoelectric point
4.9
Predicted Molecular Mass
40.5kDa
Accurate Molecular Mass
44kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of NIS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for NIS recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB.

(May be suitable for use in other assays to be determined by the end user.)

NCBI/Uniprot data below describe general gene information for NIS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
sodium iodide symporter
NCBI Official Synonym Full Names
solute carrier family 5 (sodium/iodide cotransporter), member 5
NCBI Official Symbol
SLC5A5  [Similar Products]
NCBI Official Synonym Symbols
NIS; TDH1
  [Similar Products]
NCBI Protein Information
sodium/iodide cotransporter
UniProt Protein Name
Sodium/iodide cotransporter
UniProt Synonym Protein Names
Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5
UniProt Gene Name
SLC5A5  [Similar Products]
UniProt Synonym Gene Names
NIS; Na(+)/I(-) cotransporter; Na(+)/I(-) symporter  [Similar Products]
UniProt Entry Name
SC5A5_HUMAN
NCBI Summary for NIS
This gene encodes a member of the sodium glucose cotransporter family. The encoded protein is responsible for the uptake of iodine in tissues such as the thyroid and lactating breast tissue. The iodine taken up by the thyroid is incorporated into the metabolic regulators triiodothyronine (T3) and tetraiodothyronine (T4). Mutations in this gene are associated with thyroid dyshormonogenesis 1.[provided by RefSeq, Sep 2009]
UniProt Comments for NIS
SLC5A5: Mediates iodide uptake in the thyroid gland. Defects in SLC5A5 are the cause of thyroid dyshormonogenesis 1 (TDH1). A disorder characterized by the inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland, leading to congenital hypothyroidism. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: integral to membrane; plasma membrane; nucleus; vesicle

Molecular Function: iodide transmembrane transporter activity; sodium:iodide symporter activity

Biological Process: transport; iodide transport; thyroid hormone generation; ion transport; transmembrane transport

Disease: Thyroid Dyshormonogenesis 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with NIS recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4 publications with NIS and Brain
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