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NKB elisa kit :: Human Neurokinin B ELISA Kit

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Catalog # MBS760185
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Neurokinin B (NKB), ELISA Kit

Popular Item
Full Product Name

Human Neurokinin B ELISA Kit

Product Synonym Names
NKB
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS760185 COA
OMIM
162330
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of NKB. No significant cross-reactivity or interference between NKB and analogues is observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
15.625-1000pg/ml
Sensitivity
<9.375pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NKB were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NKB were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of NKB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forNKBpurchase
MBS760185 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neurokinin B (NKB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NKB. The ELISA analytical biochemical technique of the MBS760185 kit is based on NKB antibody-NKB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NKB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NKB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
NKB elisa kit
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with NKB. During the reaction, NKB in the sample or standard competes with a fixed amount of NKB on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to NKB. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NKB in the samples is then determined by comparing the O D of the samples to the standard curve.

Typical Standard Curve/Testing Data of NKB elisa kit
NKB elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS760185. Click to request current manual
NCBI/Uniprot data below describe general gene information for NKB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,358 Da
NCBI Official Full Name
neurokinin B
NCBI Official Synonym Full Names
tachykinin 3
NCBI Official Symbol
NCBI Official Synonym Symbols
NKB; HH10; NKNB; PRO1155; ZNEUROK1
  [Similar Products]
NCBI Protein Information
tachykinin-3
UniProt Protein Name
Tachykinin-3
UniProt Synonym Protein Names
ZNEUROK1
UniProt Gene Name
UniProt Synonym Gene Names
NKNB; NKB  [Similar Products]
UniProt Entry Name
TKNK_HUMAN
NCBI Summary for NKB
This gene encodes a member of the tachykinin family of secreted neuropeptides. The encoded preproprotein is proteolytically processed to generate the mature peptide, which is primarily expressed in the central and peripheral nervous systems and functions as a neurotransmitter. This peptide is the ligand for the neurokinin-3 receptor. This protein is also expressed in the outer syncytiotrophoblast of the placenta and may be associated with pregnancy-induced hypertension and pre-eclampsia. Mutations in this gene are associated with normosmic hypogonadotropic hypogonadism. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
UniProt Comments for NKB
TAC3: Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Belongs to the tachykinin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: extracellular region; extracellular space

Molecular Function: protein binding; receptor binding

Biological Process: female pregnancy; neuropeptide signaling pathway; tachykinin signaling pathway

Disease: Hypogonadotropic Hypogonadism 10 With Or Without Anosmia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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